Optimization of culture medium composition for manganese peroxidase and tyrosinase production during Reactive Black 5 decolourization by the yeast <i>Trichosporon akiyoshidainum</i>

Martorell, María M.; Pajot, Hipólito F.; Rovati, José I.; Figueroa, Lucía I. C. de

doi:10.1002/yea.2896

Cited by 26 publications

(12 citation statements)

References 24 publications

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“…The results of the experimental design were studied and interpreted using MINITAB 17 (PA, USA) statistical software to estimate the response of the dependent variable (Martorell et al, 2012).…”

Section: Experimental Design and Statistical Analysismentioning

confidence: 99%

Versatility of Streptomyces sp. M7 to bioremediate soils co-contaminated with Cr(VI) and lindane

Aparicio

Solá

Benimeli

et al. 2015

Ecotoxicology and Environmental Safety

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Section: Experimental Design and Statistical Analysismentioning

confidence: 99%

Versatility of Streptomyces sp. M7 to bioremediate soils co-contaminated with Cr(VI) and lindane

Aparicio

Solá

Benimeli

et al. 2015

Ecotoxicology and Environmental Safety

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“…3, near here), we attribute this effect to the lower sulfur concentrations in media where (NH 4 ) 2 SO 4 was substituted by urea as nitrogen source. Magnesium deficiency could also be detrimental to growth and decolorization by itself, as Mg is a known cofactor for many enzymes associated with dye decolorization [20,21]. Consequently, lactose, urea, yeast extract, KH 2 PO 4 and MgSO 4 were selected as media components for further optimization.…”

Section: Effect Of Carbon Nitrogen Sources and Trace Elements On Dementioning

confidence: 99%

Optimization and mechanisms for biodecoloration of a mixture of dyes by Trichosporon akiyoshidainum HP 2023

Martorell

Soro

Pajot

et al. 2017

Environmental Technology

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Trichosporon akiyoshidainum HP2023 is a basidiomycetous yeast isolated from Las Yungas rainforest (Tucumán, Argentina) and selected based on its outstanding textile-dye-decolorizing ability. In this work, the decolorization process was optimized using Reactive Black 5 as dye model. Lactose and urea were chosen as carbon and nitrogen sources through a one-at-time approach. Afterwards, factorial designs were employed for medium optimization, leading to the formulation of a simpler optimized medium which contains in g L: lactose 10, yeast extract 1, urea 0.5, KHPO 1 and MgSO 1. Temperature and agitation conditions were also optimized. The optimized medium and incubation conditions for dye removal were extrapolated to other dyes individually and a mixture of them. Dye removal process happened through both biosorption and biodegradation mechanisms, depending primarily on the dye structure. A positive relation between initial inoculum and dye removal rate and a negative relation between initial dye concentration and final dye removal percentages were found. Under optimized conditions, T. akiyoshidainum HP2023 was able to completely remove a mixture of dyes up to a concentration of 300 mg L, a concentration much higher than those expected in real effluents.

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“…For example, tyrosinases can be applied in detoxification of phenol-containing wastewater and contaminant soils [10], synthesis of L-3,4-dihydroxyphenylalanine (L-DOPA), one of the preferred drugs for the treatment of Parkinson‘s disease [11], or as additives in food processes due to their cross-linking abilities [12,13]. Tailoring polymers, e.g.…”

Section: Introductionmentioning

confidence: 99%

High level production of tyrosinase in recombinant Escherichia coli

et al. 2013

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BackgroundTyrosinase is a bifunctional enzyme that catalyzes both the hydroxylation of monophenols to o-diphenols (monophenolase activity) and the subsequent oxidation of the diphenols to o-quinones (diphenolase activity). Due to the potential applications of tyrosinase in biotechnology, in particular in biocatalysis and for biosensors, it is desirable to develop a suitable low-cost process for efficient production of this enzyme. So far, the best production yield reported for tyrosinase was about 1 g L-1, which was achieved by cultivating the filamentous fungus Trichoderma reesei for 6 days.ResultsIn this work, tyrosinase from Verrucomicrobium spinosum was expressed in Escherichia coli and its production was studied in both batch and fed-batch cultivations. Effects of various key cultivation parameters on tyrosinase production were first examined in batch cultures to identify optimal conditions. It was found that a culture temperature of 32 °C and induction at the late growth stage were favorable, leading to a highest tyrosinase activity of 0.76 U mL-1. The fed-batch process was performed by using an exponential feeding strategy to achieve high cell density. With the fed-batch process, a final biomass concentration of 37 g L-1 (based on optical density) and a tyrosinase activity of 13 U mL-1 were obtained in 28 hours, leading to a yield of active tyrosinase of about 3 g L-1. The highest overall volumetric productivity of 103 mg of active tyrosinase per liter and hour (corresponding to 464 mU L-1 h-1) was determined, which is approximately 15 times higher than that obtained in batch cultures.ConclusionsWe have successfully expressed and produced gram quantities per liter of active tyrosinase in recombinant E. coli by optimizing the expression conditions and fed-batch cultivation strategy. Exponential feed of substrate helped to prolong the exponential phase of growth, to reduce the fermentation time and thus the cost. A specific tyrosinase production rate of 103 mg L−1 h−1 and a maximum volumetric activity of 464 mU L−1 h-1 were achieved in this study. These levels have not been reported previously.

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Optimization of culture medium composition for manganese peroxidase and tyrosinase production during Reactive Black 5 decolourization by the yeast Trichosporon akiyoshidainum

Cited by 26 publications

References 24 publications

Versatility of Streptomyces sp. M7 to bioremediate soils co-contaminated with Cr(VI) and lindane

Versatility of Streptomyces sp. M7 to bioremediate soils co-contaminated with Cr(VI) and lindane

Optimization and mechanisms for biodecoloration of a mixture of dyes by Trichosporon akiyoshidainum HP 2023

High level production of tyrosinase in recombinant Escherichia coli

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