Optimization of dilute acid-based pretreatment and application of laccase on apple pomace

Parmar, Indu; Rupasinghe, H.P. Vasantha

doi:10.1016/j.biortech.2012.07.030

Cited by 28 publications

(15 citation statements)

References 29 publications

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“…The highest amounts of those compounds were found in nitric acid hydrolysate (217.5, 111.6, 53.6, 22.9 and 16.3 µg mL -1 , respectively). These results were not in accordance with those obtained by Parmar et al [37] that determined chlorogenic, caffeic and ferulic acids as main phenolic compounds in hydrolysates of AP pretreated with dilute sulfuric acid. This difference could be due to the big variety in the composition of apples (types of apples and cropland).…”

Section: Methods Applicationcontrasting

confidence: 99%

“…In this way, those compounds were found in steampretreated hydrolysate of willow impregnated with SO2 [35], in alkaline wet oxidation hydrolysate of wheat straw [15] or in dilute sulfuric acid hydrolysates of several grasses, softwoods, hardwoods and agaves [36]. Regarding the biomasses studied in the present work, the presence of vanillic, ferulic, p-coumaric, p-hydroxybenzoic and syringic acids has been previously reported in alkaline hydrolysate of BSG [5] and caffeic acid and ferulic acid in dilute sulfuric acid hydrolysate of AP [37]. Nevertheless, no detailed studies based on the identification of phenolic compounds in hydrolysates of AP, PP, BSG or CS has been found in literature.…”

Section: Identification (Gc-ms) Of Potential Fermentation Inhibitory supporting

confidence: 67%

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Development and validation of a HPLC-DAD method for simultaneous determination of main potential ABE fermentation inhibitors identified in agro-food waste hydrolysates

Paniagua-García

Hijosa‐Valsero

Garita-Cambronero

et al. 2019

Microchemical Journal

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Lignocellulosic agro-food wastes are regarded as interesting carbohydrate sources for acetone-butanol-ethanol (ABE) fermentation. However, the physicochemical and enzymatic pretreatments applied to release their sugars generate inhibitory compounds that hinder the fermentation. The release of inhibitory compounds in the hydrolysates of four agro-food industrial wastes [apple pomace (AP), potato peel (PP), brewers' spent grain (BSG) and coffee silverskin (CS)] obtained after various chemical pretreatments (acid, alkali, organic solvents and surfactant pretreatments) was analyzed. Sixty-seven potential inhibitors were identified by gas chromatographymass spectrometry (GC-MS) and were classified into non-aromatic compounds (aliphatic acids, nitrogen-containing compounds, furans and fatty acids) and aromatic compounds (phenolics and non-phenolics). Then, a high performance liquid chromatography (HPLC) method with diode array detection (DAD) was developed and validated for the quantification of the main potential inhibitors identified in the hydrolysates (i.e. gallic, 3,4-dihydroxybenzoic, 2,5-dihydroxibenzoic, 4-hydroxybenzoic, 3-hydroxybenzoic, vanillic, caffeic, syringic, p-coumaric, and ferulic acids, vanillin, syringaldehyde and caffeine). The proposed HPLC-DAD method was simple, fast and robust and allowed the direct injection of samples without previous preparation, enabling the simultaneous quantification of the abovementioned compounds for the first time. The method was successfully applied to the analysis of AP, PP, BSG and CS hydrolysates.

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Section: Methods Applicationcontrasting

confidence: 99%

Section: Identification (Gc-ms) Of Potential Fermentation Inhibitory supporting

confidence: 67%

Development and validation of a HPLC-DAD method for simultaneous determination of main potential ABE fermentation inhibitors identified in agro-food waste hydrolysates

Paniagua-García

Hijosa‐Valsero

Garita-Cambronero

et al. 2019

Microchemical Journal

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“…The organic acids may also inhibit microbial fermentation by inhibiting their growth and metabolism (e.g. formic acid and acetic acid have been reported to have an inhibitory effect on microbial fermentation) (Parmar and Rupasinghe 2012). Butyric, acetic and citric acid only had a slightly inhibitory effect on enzyme activity, with activity remaining above 80 %, even at concentrations of 10 g/L.…”

Section: Resultsmentioning

confidence: 99%

“…In the case of apple pomace, the hemicellulose composition has not been studied and therefore it is not clear which enzymes are required. Some authors have indicated that apple pomace contains xylan (Bhushan et al 2008; Dhillon et al 2012; Parmar and Rupasinghe 2012), which would indicate a requirement for endoxylanases and β-xylosidases, as well as possible debranching enzymes. However, the xylose found in apple pomace could also be present in the form of xyloglucan or xylogalacturonan.…”

Section: Introductionmentioning

confidence: 99%

Optimisation of enzymatic hydrolysis of apple pomace for production of biofuel and biorefinery chemicals using commercial enzymes

2015

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Apple pomace, a waste product from the apple juice industry is a potential feedstock for biofuel and biorefinery chemical production. Optimisation of hydrolysis conditions for apple pomace hydrolysis using Viscozyme L and Celluclast 1.5L was investigated using 1 L reaction volumes. The effects of temperature, pH, β-glucosidase supplementation and substrate feeding regimes were determined. Hydrolysis at room temperature using an unbuffered system gave optimal performance. Reactors in batch mode resulted in a better performance (4.2 g/L glucose and 16.8 g/L reducing sugar, 75 % yield for both) than fed-batch (3.2 g/L glucose and 14.6 g/L reducing sugar, 65.5 and 73.1 % yield respectively) in 72 h. The addition of Novozyme 188 to the core mixture of Viscozyme L and Celluclast 1.5L resulted in the doubling of glucose released. The main products (yield %) released from apple pomace hydrolysis were galacturonic acid (78 %), glucose (75 %), arabinose (90 %) and galactose (87 %). These products are potential raw materials for biofuel and biorefinery chemical production.

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“…It has been used in other recent research on laccase production (Lee et al, 2012;Parmar et al, 2012). To maximize laccase production and understand the effects of interacting variables, we optimized the fermentation medium and fermentation conditions for laccase production in shake-flask cultures using two different RSMs.…”

Section: Response Surface Methodologymentioning

confidence: 99%

Laccase production by Phomopsis liquidambari B3 cultured with food waste and wheat straw as the main nitrogen and carbon sources

Zhou

Yang

Mei

et al. 2014

Journal of the Air & Waste Management Association

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Food waste is the most difficult waste to manage because of the high treatment costs and the risk of environmental contamination. Wheat straw burning has become an increasingly discussed topic in recent years because of the air pollution it causes. However, food waste and wheat straw contain high amounts of nutrient elements; efficient utilization of these wastes can improve the environment by lessening airborne pollutant emission and the amount of waste that must be landfilled. It is well known that use of low-cost and abundant waste materials in microbial fermentations can reduce product costs. We aimed to use these resources while improving laccase production by the endophytic fungus Phomopsis liquidambari B3. We cultured P. liquidambari B3 in medium containing food waste and wheat straw as the main nitrogen and carbon sources, respectively. We optimized the fermentation conditions by response surface methodology (RSM), using a Box-Behnken design for RSM I and a central composite design for RSM II. Optimization resulted in an 11.07-fold (1.98-fold RSM I; 5.59-fold RSM II) increase in laccase yield compared with that before optimization. The model was validated by mathematical evaluations and by comparisons between predicted and experimental values. Under optimized conditions, 53.76% of lignin in wheat straw was degraded. By optimizing fermentation conditions and using multiple bioresources, laccase production by this fungus was increased. These results provide the foundation for future research and for scaled-up laccase production.Implications: Food waste and wheat straw are waste products, but they could be bioresources if they were managed properly. In this work, we innovatively used a mixture of food waste and wheat straw as a substrate for the novel strain Phomopsis liquidambari B3 to produce laccase. This process, which provided the foundation for the subsequent research and for scaled-up laccase production, offers solutions for both rural and urban pollution problems: that is, it reduces the amount of waste material that needs to be disposed of by burning or dumping, and it also produces a valuable product.

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Optimization of dilute acid-based pretreatment and application of laccase on apple pomace

Cited by 28 publications

References 29 publications

Development and validation of a HPLC-DAD method for simultaneous determination of main potential ABE fermentation inhibitors identified in agro-food waste hydrolysates

Development and validation of a HPLC-DAD method for simultaneous determination of main potential ABE fermentation inhibitors identified in agro-food waste hydrolysates

Optimisation of enzymatic hydrolysis of apple pomace for production of biofuel and biorefinery chemicals using commercial enzymes

Laccase production by Phomopsis liquidambari B3 cultured with food waste and wheat straw as the main nitrogen and carbon sources

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