Optimization of Electrochemical Parameters for Specific Blood Methylglyoxal Determination Using ZnO Sepals Based Glyoxalase 1 Biosensor

Fabrication of an electrochemical biosensor with ZnO nanoflakes interface for methylglyoxal quantification in food samples

“…In the past, the expensive and time consuming complex procedures like gas chromatography-mass spectrometry and high-performance liquid chromatography [13] were developed to detect MG in food products. Recently, nonenzymatic MG electrochemical biosensors were developed by using single walled carbon nanotubes (SWCNTs) and platinum (Pt) nanoparticles [14,15].…”

“…However, specificity and precision were less in these types of electrochemical biosensors. Whereas, in the case of enzyme-based electrochemical biosensors [13,[16][17][18][19][20][21], high specificity, accuracy and rapidness can be achieved. They were used extensively to detect biological molecules and toxins in clinical diagnosis, food products and in the environment.…”

Estimation of methylglyoxal in cow milk – an accurate electrochemical response time based approach

“…The conventional methods used for the detection of MG are high performance liquid chromatography (HPLC), gas chromatography, 7 GC-MS, 8 ion pair chromatography, MEKC, 9 capillary electrophoresis and enzyme-linked immunosorbent assay (ELISA), which are time consuming and expensive. [10][11][12][13] Electrochemical biosensors, which are simple, highly selective, low-cost, rapid and portable, have been proposed for methylglyoxal detection. [10][11][12][13][14][15] Various nanomaterials such as zinc oxide (ZnO) nanosepals and akes, platinum (Pt) nanoparticles and single wall carbon nanotubes (SWCNT) have been employed as effective immobilization matrices for the development of highly sensitive MG biosensors.…”

“…[10][11][12][13] Electrochemical biosensors, which are simple, highly selective, low-cost, rapid and portable, have been proposed for methylglyoxal detection. [10][11][12][13][14][15] Various nanomaterials such as zinc oxide (ZnO) nanosepals and akes, platinum (Pt) nanoparticles and single wall carbon nanotubes (SWCNT) have been employed as effective immobilization matrices for the development of highly sensitive MG biosensors. [10][11][12][13] Among them, the ZnO nanosepals and akes were used as interfaces for glyoxalase 1 (GLO 1) immobilization towards the detection of MG in blood and grilled chicken respectively.…”

“…[10][11][12][13][14][15] Various nanomaterials such as zinc oxide (ZnO) nanosepals and akes, platinum (Pt) nanoparticles and single wall carbon nanotubes (SWCNT) have been employed as effective immobilization matrices for the development of highly sensitive MG biosensors. [10][11][12][13] Among them, the ZnO nanosepals and akes were used as interfaces for glyoxalase 1 (GLO 1) immobilization towards the detection of MG in blood and grilled chicken respectively. Whereas, Pt and SWCNT play the role of glutathione (GSH), which is involved in the direct catalysis of MG to hemithioacetal.…”