Optimization of Electrohydrodynamic Writing Technique to Print Collagen

Kim, Hak-Sub; Lee, D.-Y.; Park, Jongho; Kim, J.-H.; Hwang, Jungho; Jung, Hyo Il

doi:10.1111/j.1747-1567.2007.00154.x

Cited by 34 publications

(16 citation statements)

References 14 publications

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“…25,[27][28][29]38 To address and understand the importance of surface/network topology on cell growth and function, nano/microscale fabrication techniques are required. Dip-pen techniques have shown it possible to create submicron surface features of thiolated collagen by transfer from an atomic force microscopy tip onto a solid support and in doing so provided a new level of control for direct writing of biomaterial.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Microfabrication of Custom Collagen Structures Capable of Guiding Cell Morphology and Alignment

2015

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The patterning of biological components into structural analogues of native tissues to simulate an environment for directing cell growth is one important strategy in biomaterials fabrication. It is widely accepted that chemical, mechanical, and topological cues from the extracellular matrix (ECM) provide important signals for guiding cells to exhibit characteristic polarity, orientation, and morphology. To fully understand the delicate relationship between cell behavior and ECM features, biomaterials fabrication requires improved techniques for tailoring nano/microstructured patterns from relevant biological building blocks rather than using nonbiological materials. Here we reveal a unique approach for the nano/microfabrication of custom patterned biomaterials using collagen as the sole building material. With this new fabrication technique, we further revealed that custom collagen patterns could direct the orientation and morphology of fibroblast growth as a function of vertex density and pattern spacing. Our findings suggest that this technique may be readily adopted for the free form fabrication of custom cell scaffolds purely from natural biological molecules including collagen, among other relevant ECM components.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Microfabrication of Custom Collagen Structures Capable of Guiding Cell Morphology and Alignment

2015

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“…Consequently, bioink droplets are ejected when the electrostatic stresses overcome the surface tension at the orifice under a sufficiently high voltage depending on the process parameters. The strength of the electric field (applied voltage), the bioink flow rate and the bioink properties (including cell type and concentration [69]) determine the jetting mode [42,70] and cell viability [71]. Dripping mode is observed at low voltages and flow rates, Although process parameters do not significantly affect the cell viability (see Table 2) and genomic expression immediately after bioprinting, applied voltage, cell concentration and bioink constituents can collectively affect the long-term post-bioprinting cell viability [71].…”

Section: Electrohydrodynamic Jet Bioprintingmentioning

confidence: 99%

A comprehensive review on droplet-based bioprinting: Past, present and future

2016

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Droplet-based bioprinting (DBB) offers greater advantages due to its simplicity and agility with precise control on deposition of biologics including cells, growth factors, genes, drugs and biomaterials, and has been a prominent technology in the bioprinting community. Due to its immense versatility, DBB technology has been adopted by various application areas, including but not limited to, tissue engineering and regenerative medicine, transplantation and clinics, pharmaceutics and high-throughput screening, and cancer research. Despite the great benefits, the technology currently faces several challenges such as a narrow range of available bioink materials, bioprinting-induced cell damage at substantial levels, limited mechanical and structural integrity of bioprinted constructs, and restrictions on the size of constructs due to lack of vascularization and porosity. This paper presents a first-time review of DBB and comprehensively covers the existing DBB modalities including inkjet, electrohydrodynamic, acoustic, and micro-valve bioprinting. The recent notable studies are highlighted, the relevant bioink biomaterials and bioprinters are expounded, the application areas are presented, and the future prospects are provided to the reader.

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“…7(a). The line that exhibits rivulet fluctuation [9] owing to the good fluidity is printed in the contact mode [7]. No cone-jet mode is generated due to the low viscosity [11] of 3wt% solution.…”

Section: Concentration Of the Solutionmentioning

confidence: 99%

Bead-on-string structure formed by electrohydrodynamic printing

Huang

Zheng

et al. 2010

2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems

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A Bead-on-String (B-S) structure which consists of droplet and filament is generated based on electrohydrodynamic printing system. The formation process of the B-S structure is demonstrated and discussed. Subsequently, the influence of the substrate moving speed on the B-S structure is investigated. The size of the droplet in the B-S structure decreases with the substrate moving speed. When the substrate moving speed is higher than the jetting speed, satellite droplet will appear and its number increases with the substrate moving speed. The effect of the solution concentration on the deposited patterns is also studied. A continuous line is printed with 3 wt% PEO solution. The B-S structure is generated when the concentration of the PEO solution is within 5-15 wt%. Moreover, the size of the droplet decreases with the increasing of the solution concentration. At the concentration of 18 wt%, nanofiber is produced and a pattern similar to the B-S structure is deposited on the substrate.

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Optimization of Electrohydrodynamic Writing Technique to Print Collagen

Cited by 34 publications

References 14 publications

Microfabrication of Custom Collagen Structures Capable of Guiding Cell Morphology and Alignment

Microfabrication of Custom Collagen Structures Capable of Guiding Cell Morphology and Alignment

A comprehensive review on droplet-based bioprinting: Past, present and future

Bead-on-string structure formed by electrohydrodynamic printing

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