Optimization of enzymatic hydrolysis conditions for the production of antioxidant peptides from muscles of Nemipterus japonicus and Exocoetus volitans using response surface methodology

Abstract

In the present study, protein of muscles of commercially important marine fishes Nemipterus japonicus and Exocoetus volitans were extracted by trypsin and their hydrolysis conditions viz., temperature, time, and enzyme to substrate concentration on degree of hydrolysis were studied by response surface methodology. The optimum values for N. japonicus was found as temperature, 30°C, hydrolysis time of 100 min an enzyme/substrate concentration of 1.59% whereas, for E. volitans muscle protein, optimum hydrolysis conditions were temperature, 30°C, hydrolysis time of 115 min and enzyme/substrate concentration of 1.67%. Furthermore, amino acid sequence of antioxidant peptides derived after chromatographic purification was identified by ESI-MS/MS. The analysis of peptides showed sequences as Glu-Ser-Asp-Arg-Pro (620.3 Da) and Gly-Trp-Met-Gly-Cys-Trp (747.3) for N. japonicus and E. volitans muscle, respectively. The peptides contained important antioxidant amino acids and acted as good antioxidant peptides to scavenge free radicals.

Roe protein hydrolysate of Alburnus tarichi induces apoptosis in breast cancer MCF-7 and MDA-MB-231 cells through a caspase-dependent pathway

“…The IC 50 value of the present study was comparably lower than the reported value of Naqash and Nazeer (2012) for Nemipterus japonicus (48.5 μg/ml) and Exocoetus volitans (21.6 μg/ml) muscle protein hydrolysates in liver carcinoma cell line (Hep-G2). Further, Alemán et al (2011) reported the IC 50 values of esperase and alcalase squid gelatin hydrolysates on MCF-7 cells at 24 h as 0.15 mg/ml and 1.18 mg/ml, respectively.…”

Purification, Characterization, and Application for Preparation of Antioxidant Peptides of Extracellular Protease from Pseudoalteromonas sp. H2

“…Based on the DPPH radicals, hydroxyl radicals, and ORAC assays, the two hydrolysis products exerted the best effects when hydrolyzed for 3 h. After 3 h, the antioxidant activity began to decline. The reason may have been that some of the antioxidant peptides were further hydrolyzed with increasing reaction time, given that low molecular peptides contribute more to the inhibitory activity than polypeptides [12,23]. In addition, DPPH and hydroxyl radical scavenging activity of salmon skin collagen hydrolysates were better than those of porcine skin collagen hydrolysates, but as for the absorption capability of against peroxyl radical, the latter had higher efficiency.…”

“…However, they are often discarded as byproducts of the meat processing industries [10] and cause serious waste of protein and environmental problems. Many studies have reported that enzymatic hydrolysis of collagen is an effective way to obtain antioxidant peptides [11,12]. Most of the proteases used in these studies are commercially available, such as trypsin, pepsin, α-chymotrypsin, papain, Protamex, and neutral protease.…”

Optimization of Enzymatic Hydrolysis for Producing Ferrous Binding Peptides from Horse Mackerel (Trachurus japonicus) Processing Byproducts

“…Choice of the enzyme: Suitable enzymes can be selected for marine protein hydrolysis according to various criteria, such as high DH (Dey and Dora, 2014), bioactive peptides (Chabeaud et al, 2009;Naqash and Nazeer, 2012;Dadzie et al, 2013), or reduction of bitterness (Nilsang et al, 2005;Sumaya-Martinez et al 2005). In this study, ferrous binding ability was selected to evaluate the hydrolysis effective of various proteases for horse mackerel processing byproducts.…”