Optimization of extracellular endoxylanase, endoglucanase and peroxidase production by Streptomyces sp. F2621 isolated in Turkey

Abstract

Aims: To determine the effect of environmental conditions on the production of extracellular lignocellulose‐degrading enzymes by Streptomyces sp. F2621 and to assess the potential use of these enzymes in the hydrolysis of lignocellulose material. Methods and Results: The production of extracellular lignocellulose‐degrading enzymes, endoxylanase, endoglucanase and peroxidase during the growth of Streptomyces sp. F2621 in basal salts‐yeast extract medium containing different carbon sources and the effect of a number of environmental parameters (e.g. carbon sources and concentrations, pH and temperature) were investigated. The highest endoxylanase (22·41 U ml−1) and peroxidase (0·58 U ml−1) activities were obtained after 2–4 days of incubation at 30°C in a basal salts medium containing 0·4% (w/v) oat spelt xylan and 0·6% (w/v) yeast extract, corresponding to C : N ratio of 6 : 1. Cell‐free extracellular enzyme preparations from the strain were capable of releasing both sugar and aromatic compounds during incubation with eucalyptus paper pulp, straw and xylan. Overall, 9·3% hydrolysis of xylan occurred after 24‐h incubation. However the rates of hydrolysis of paper pulp and straw were approximately twofold less than xylan hydrolysis, although the total percentage hydrolysis of available substrate (24·5% and 16·3%, respectively) was greater than xylan hydrolysis. Conclusions: The high levels of enzyme production achieved under batch cultivation conditions, coupled with no significant production of endoglucanase during the growth phase of organism and the release of both sugar and aromatic compounds from paper pulp and straw signify the suitability for these enzymes for industrial applications such as pulp and paper production. Significance and Impact of the Study: The results highlight the environmental conditions for the production of extracellular lignocellulose‐degrading enzymes by Streptomyces sp. F2621 and suggest the use of streptomycetes and/or their enzymes in industrial processes.

Production of endoglucanase by the native strains of Streptomyces isolates in submerged fermentation

“…Similarly, Streptomyces sp. F2621 (19) and S. albogriseous (20) also exhibited maximum endoglucanase activity at 26-30ºC with initial pH of 6.5-7.0.…”

“…strain M7a & strain M7b (17), and Streptomyces sp. F2621 (19) have already reported to produce extracellular endoglucanase significantly in optimized culture media using cellulose powder as carbon source under shaking conditions. Culture conditions, therefore, play a prominent role on formation of endoglucanase from bacteria (10,11).…”

Cloning and characterization of an endo--1,4-xylanase gene from Colletotrichum lindemuthianum and phylogenetic analysis of similar genes from phytopathogenic fungus

“…Accordingly, though maximum growth was obtained on glucose, a readily metabolizable nutrient, basal expression of xyl1 and only trace amounts of XYL were produced by both races; however, although low, a higher XYL production was observed for race 1472, particularly early during incubation. A similar basal production of xylanase and endoglucanase (Sachslehner et al, 1998;Tuncer et al, 2004), pectinases (Hernández-Silva et al, 2007;Oyeleke et al, 2012;Riou et al, 1991) and cellulases (AcostaRodriguez et al, 2005;Carle-Urioste et al, 1997;Sharada et al, 2013) has been described in the fungi S. rolsfii, S. sclerotiorum and C. lindemuthianum, respectively.…”

Medium optimization for the production of avermectin B1a by Streptomyces avermitilis 14-12A using response surface methodology

“…In previous experiments, it was found that yeast extract is the preferred nitrogen source of Streptomyces strains (Gill et al, 2003;Lazim et al, 2009;Tuncer et al, 2004). However, every coin has two sides; it is also true for yeast extract in some Streptomyces strains (Niladevi et al, 2009).…”