Production of endoglucanase by the native strains of Streptomyces isolates in submerged fermentation

Abstract

Cellulase is a complex enzyme system, commercially produced by filamentous fungi under solid-state and submerged cultivation. It has wide applicability in textile, food and beverage industry for effective saccharification process. In this study, cellulolytic enzyme activity, particularly endoglucanase of 26 Streptomyces strains isolated from garden soil was examined, including two isolates selected on the basis of potential cellulolytic activity on Bennett's agar medium. To enhance the endoglucanase formation in broth culture, different conditions including carbon and nitrogen sources, and growth conditions were tested. The maximum endoglucanase activity (11.25-11.90 U/mL) was achieved within 72-88 h in fermentation medium containing Tween-80, followed by phosphate sources. Both cellulolytic Streptomyces isolates gave almost equal quantity of enzyme in all trials. However the effect of medium ingredients on endoglucanase induction diverged with strains in some extent.

Kinetic of Biomass Growth and Protein Formation on Rice Bran Fermentation Using Rhizopus oryzae

“…Table 1 it can be seen that after fermentation for 120 hours there was a decrease of carbohydrate and fiber content respectively 25.6% and 51.2%, this is due to activity of Rhizopus oryzae metabolism that grow and developed in rice bran. Rhizopus oryzae produces enzymes used to break down polysaccharides such as cellulase, xylanase, pectinase, and amylase to oligosaccharides and glucose, resulting in reduced carbohydrate and fiber content [22,23,25,26,42].…”

“…Various types of enzymes produced by various strains of Rhizopus oryzae include cellulase which serves to break down cellulose into oligosaccharides, glucose or other compounds. Another enzyme produced is xylanase which catalyzes the hydrolysis of 1-4-beta-D-glycosidic on xylans which is the constituent of hemicellulose, pectinase which serves to break down pectin into simple sugars, and amylase [22][23][24][25][26][27].…”

Biocatalysis of agro-processing waste by marine Streptomyces fungicidicus strain RPBS-A4 for cellulase production

“…This demonstrates that incubation time prominently affected cellulase production by the strain and the ideal incubation time for cellulase generation is 96 h. Cellulase activity of S. fungicidicus RPBS-A4 (12.29 U/ml) under the optimized conditions is especially greater than the activity of mentioned strains Streptomyces sp. BRC1 (6.4 U/ml) and BRC2 (6.6 U/ml) isolated from the soil of decomposed garden [3].…”

“…Temperature assumes an essential part in the development of actinomycetes; under the ideal temperature, the development rate of S. fungicidicus RPBS-A4 was expanded, as the development rate expanded cellulase generation was additionally expanded. Chellapandi and Jani [3] declared that the highest quantity of enzyme was produced by Streptomyces sp. BRC1 and BRC2 in submerged fermentation at ideal temperature 26°C, i.e.…”

“…Strain M7a and strain M7b and Streptomyces sp. F2621 have just been accounted for creating extracellular endoglucanase altogether in upgraded culture media utilizing cellulose powder as substrate [3]. However, a detailed investigation on the development of this enzyme from Streptomyces species has been hindered.…”