Optimization of extracellular psychrophilic alkaline lipase produced by marine Pseudomonas sp. (MSI057)

Kiran, George Seghal; Shanmughapriya, Santhanam; Jayalakshmi, J.; Selvin, Joseph; Gandhimathi, R.; Sivaramakrishnan, S.; Arunkumar, M.; Thangavelu, Thayumanavan; Natarajaseenivasan, Kalimuthusamy

doi:10.1007/s00449-007-0186-0

Cited by 73 publications

(46 citation statements)

References 43 publications

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“…Increasing or decreasing the temperature causes a decrease in enzyme units showing that P. putida is a mesophilic bacterium 30°C. Similar results have been shown by Padhiar et al [15] for P. aeruginosa and Kiran et al [16] who found 30°C as the optimum temperature for enzyme production from Pseudomonas sp.…”

Section: Discussionsupporting

confidence: 88%

Production and Partial Characterization of Lipase from Pseudomonas putida

Fatima¹,

Khan²

2015

Ferment Technol

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The production of lipase from Pseudomonas putida 922 was optimized by modifying various physical parameters such as carbon source, nitrogen source, pH, salt concentration and biochemical parameters of the production medium such as temperature and incubation time of the growth medium. Oil cakes were also used as carbon source to check for an increased production of the enzyme. The bacterium was found to have a maximal growth at pH 10 with the enzyme production being highest (24 U/ml) after 48 hours at 30°C and pH 10. The optimum composition of the medium was mustard oil cake as carbon source, yeast extract or peptone as nitrogen source and 1% sodium chloride concentration. Partial characterization of the enzyme was carried out where the optimum working pH and temperature was found to be 10 and 40ºC, respectively. Enzyme stability was found to lie in the pH and temperature ranges of 5-11 and 30-40ºC, respectively. Partial purification of the enzyme was carried out at 80% ammonium sulphate saturation. Molecular mass of lipase was determined by SDS PAGE and found to be 45 kDa.

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Section: Discussionsupporting

confidence: 88%

Production and Partial Characterization of Lipase from Pseudomonas putida

Fatima¹,

Khan²

2015

Ferment Technol

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“…Aeromonas hydrophila and Pseudomonas sp. MSI057 were isolated from marine environment and marine sponge, respectively (Pemberton et al 1997;Kiran et al 2008). Recently, Janibacter sp.…”

Section: Sources Of Clpsmentioning

confidence: 99%

Cold active lipases – an update

Kavitha

2016

Frontiers in Life Science

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Cold active lipases (CLPs) are gaining importance nowadays as they are increasingly used in fine chemical synthesis, bioremediation, food processing and as detergent additive. These enzymes exhibit high catalytic activity at low temperatures and flexibility to act at low water medium. Since they are active at low temperatures consume less energy and also stabilize fragile compounds in the reaction medium. CLPs are commonly obtained from psychrophilic microorganisms which thrive in cold habitats. Compared to mesophilic and thermophilic lipases, only a few CLPs were studied and industrially exploited so far. CLPs (C. antarctica lipase-A and C. antarctica lipase-B) from Candida antarctica isolated from Antarctic region are the well studied and industrially employed, and many are being followed up. This review updates the CLPs reported recently and the industrial applications of CLPs. ARTICLE HISTORY

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“…The maximum production of lipase (3.69 U/mL) was obtained at pH of 8.0 (Figure 7). This might be because organism required a slightly basic pH for its metabolic processes as well as for the production of lipases (Kiran et al, 2008). Increase or decrease in pH had negative impact on the production of lipase.…”

Section: Effect Of Ph Of the Medium On Production Of Lipase -mentioning

confidence: 99%

Screening and Optimization of Filamentous Fungi for Extracellular Lipase Production from Various Soil Samples Taken In and Around Shimla (HP), India

Kumar¹,

Faujdar²,

Mehrishi³

et al. 2017

Int.J.Curr.Res.Aca.Rev.

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Article InfoIn the present study samples were collected from different sources such as oil contaminated soil (railway station), moist soil, barren land, garden soils and humus, near dairy and other soil samples in and around Shimla. FromTotal of 48 isolates were isolated from different samples, out of which four were selected on the basis of zone of hydrolysis on tributyrin-agar plates. Among these four isolates, Isolate -3 (Aspergillus niger) gave maximum production of lipase. Further the optimization of production and reaction conditions were carried out. Enzyme gave maximum activity in M3 medium with an inoculum size of 3.6×10⁵ spores, at incubation time of 72 h. The enzyme gave considerable amount of activity with maltose and peptone as carbon and nitrogen source respectively at a concentration of 2.0% each at pH 8.0, temperature of 40°C and 150 rpm. During the optimization of reaction conditions, the most favorable reaction conditions was found to be 40°C temperature for 15 minutes of incubation and it was observed that the enzyme showed maximum enzyme activity i.e. 4.3 U/mL with Tris HCl buffer (40 mM) of pH 8.0. A 2.17 fold increase in activity of lipase from fungal Isolate -3 (Aspergillus niger) was observed after optimization of culture and reaction conditions.

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Optimization of extracellular psychrophilic alkaline lipase produced by marine Pseudomonas sp. (MSI057)

Cited by 73 publications

References 43 publications

Production and Partial Characterization of Lipase from Pseudomonas putida

Production and Partial Characterization of Lipase from Pseudomonas putida

Cold active lipases – an update

Screening and Optimization of Filamentous Fungi for Extracellular Lipase Production from Various Soil Samples Taken In and Around Shimla (HP), India

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