Production and Partial Characterization of Lipase from Pseudomonas putida

Abstract

The production of lipase from Pseudomonas putida 922 was optimized by modifying various physical parameters such as carbon source, nitrogen source, pH, salt concentration and biochemical parameters of the production medium such as temperature and incubation time of the growth medium. Oil cakes were also used as carbon source to check for an increased production of the enzyme. The bacterium was found to have a maximal growth at pH 10 with the enzyme production being highest (24 U/ml) after 48 hours at 30°C and pH 10. The optimum composition of the medium was mustard oil cake as carbon source, yeast extract or peptone as nitrogen source and 1% sodium chloride concentration. Partial characterization of the enzyme was carried out where the optimum working pH and temperature was found to be 10 and 40ºC, respectively. Enzyme stability was found to lie in the pH and temperature ranges of 5-11 and 30-40ºC, respectively. Partial purification of the enzyme was carried out at 80% ammonium sulphate saturation. Molecular mass of lipase was determined by SDS PAGE and found to be 45 kDa.

Enhancing curcumin's solubility and antibiofilm activityviasilica surface modification

“…In addition to this effect, the fact that curcumin is linked to a succinic anhydride moiety via an ester bond makes them prone to be released by the cleavage of this bond by lipases. This class of enzymes are usually abundant in many biofilms species, 69–71 including in P. putida , 72 and often have a low specificity towards their substrates. In fact, a number of studies have reported enhanced antibiofilm effect via bacterial lipase-triggered release of antibiotics.…”

Classification of lipolytic enzymes and their biotechnological applications in the pulping industry

“…Numerous microorganisms are known to produce lipases when incubated with lipid substrates (Haba et al 2000). The majority of bacterial lipases originate from Gram-negative bacteria, the most valuable being Pseudomonas, which includes at least 7 lipaseproducing species, which are P. aeruginosa (Prasad 2014;Tielen et al 2013), Pseudomonas cepacia (Badgujar et al 2016;Cao et al 2016;Sasso et al 2016), Pseudomonas alcaligenes (Chen et al 2014;Patel et al 2014), Pseudomonas glumae (Knapp et al 2016), P. fluorescens (Guldhe et al 2015;Lima et al 2015;Xun et al 2012), Pseudomonas fragi (Dey et al 2014;Santarossa et al 2005), and Pseudomonas putida (Fatima et al 2014). …”

Isolation, purification and characterization of a novel solvent stable lipase from Pseudomonas reinekei

“…A pH 7.0 (±0.2) was found to be optimum for lipase production in Pseudomonas gessardii[23], P. fluorescens[24] and P. aeruginosa[25]. In comparison, Pseudomonas putida produced maximum lipase after 48 hours of incubation in a production media at pH 10[26].…”