Optimization of extraction conditions of antioxidants from sunflower shells (Helianthus annuus L.) before and after enzymatic treatment

Szydłowska‐Czerniak, Aleksandra; Trokowski, Konrad; Szłyk, Edward

doi:10.1016/j.indcrop.2010.09.016

Cited by 30 publications

(18 citation statements)

References 26 publications

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“…Response surface methodology (RSM) has been shown to be a powerful tool in optimising experimental conditions to maximise the response. RSM is a collection of statistical techniques for designing experiments, building models, evaluating the effects of factors and searching optimal conditions for desirable responses (Szydowska-Czerniak et al, 2011). It has been successfully used in optimising the extraction of phenolic compounds from fruits, vegetables and by-products (Wardhani et al, 2010;P erez-Serradilla & Luque de Castro, 2011;Song et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Optimisation of microwave‐assisted extraction of prune (Prunus domestica) antioxidants by response surface methodology

Haddadi‐Guemghar

Janel

Dairou

et al. 2014

Int J of Food Sci Tech

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Optimal conditions for microwave-assisted extraction (MAE) of total phenols (TP), epicatechin gallate and antioxidant activity from prune (Prunus domestica), rejected in transformation process of plum to prune, were determined by response surface methodology. The central composite design was used to study the effects of three independent variables: microwave power, irradiation time and solvent polarity on the TP, epicatechin gallate and antioxidant activity. Epicatechin gallate was identified as a major phenolic compound in prune extract by RP-HPLC. Microwave power and irradiation time significantly affected all responses (P < 0.01). The highest TP (598.89 mg GAE/100 g prune) was obtained using water as an extraction solvent at 500 W, during 115 s. However, the optimal conditions for epicatechin gallate extraction were ethanol 80%, 435 W and 120 s. MAE is more efficient than conventional extraction method to obtain TP from prune. The experimental values were reasonably close to the predicted values confirming the validity of the predicted models.

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Section: Introductionmentioning

confidence: 99%

Optimisation of microwave‐assisted extraction of prune (Prunus domestica) antioxidants by response surface methodology

Haddadi‐Guemghar

Janel

Dairou

et al. 2014

Int J of Food Sci Tech

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“…The antioxidant compounds are present in both sunflower oil and oilseed-extracted meal [5]. Extracts with high antioxidant activity may also be obtained from sunflower seed shells [6,7]. Compared to vegetables oils, sunflower oil is especially rich in a-tocopherol [5].…”

Section: Introductionmentioning

confidence: 99%

Antioxidant activity of phenolic compounds identified in sunflower seeds

Karamać

Kosińska

Estrella

et al. 2012

Eur Food Res Technol

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The antioxidant activity and phenolic compound profiles of six fractions (I-VI) obtained from sunflower seed extract were studied. HPLC-MS(ESI) analysis was applied for quantitative and qualitative determination of phenolic compounds of the fractions. The antioxidant activity of the fractions was studied in terms of their ability to scavenge DPPH Á and ABTS Á? and to reduce Fe 3? /ferricyanide complex to the ferrous form and was expressed as EC 50 , TEAC and reducing power values, respectively. The results of all antioxidant activity tests showed good correlations among each other and with the phenolic contents for the individual fractions. The fractions IV-VI were characterized by high antioxidant activity. 5-O-Caffeoylquinic acid was a predominant compound of fractions IV and V, while dicaffeoylquinic acid isomers and caffeoyldimethoxycinnamoylquinic acid isomers accounted for 76.6 % of phenolic compounds of fraction VI. Ferulic acid, p-coumaroylquinic acid isomers, ferulic acid dehydrotrimer isomers and some quercetin derivatives were also identified. The highest content of those compounds was noted in fraction III.

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“…5-Caffeoylquinic acid (chlorogenic acid) is the major phenolic compound accounting for 70 % of the TPC content, while the remaining 30 % are other mono-and di-caffeoylquinic acids, as well as coumaroyl-and feruloylquinic acids. Free caffeic (CA) acid is also found in SFM but accounts for just 0.5 % of the TPC content (Guo et al, 2017;Szydłowska-Czerniak et al, 2011;Weisz et al, 2009). Phenolic acids, particularly hydroxycinnamic acids, are bioactive molecules with interesting properties that include antioxidant (Gullón et al, 2018;Shahidi and Ambigaipalan, 2015), anti-inflammatory (Oueslati et al, 2012) and antimicrobial (Ouerghemmi et al, 2017) activities among others.…”

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confidence: 99%

Release of phenolic acids from sunflower and rapeseed meals using different carboxylic esters hydrolases from Aspergillus niger

Laguna

Odinot

Bisotto

et al. 2019

Industrial Crops and Products

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Sunflower and rapeseed meals are agro-industrial coproducts that contain high amount of phenolics (1-4 % dry matter), mostly as esters of caffeic acid (CA) and sinapic acid (SA), respectively. The enzymatic hydrolysis of the ester bonds enables to recover the corresponding free phenolic acids that are bioactive compounds and platform molecules for various applications in green chemistry. Here we aimed to find the best route for producing free CA and SA by applying various fungal carboxylic ester hydrolases from recombinant Aspergillus niger strains either directly on crude meal or on their phenolic extracts obtained by methanolic extraction. Two types of meals were studied: (i) industrial (commercial) meals (I-meals), produced by a process that includes cooking at 95-100°C and steam desolventizing at 105-107°C, and (ii) non-industrial meals (NI-meals) obtained at pilot-scale with much milder heat treatment, that offer a higher total phenolic content. CA release through hydrolysis of sunflower meal (SFM) was successfully achieved with A. niger type-B feruloyl esterase (AnFaeB) and chlorogenic acid esterase (ChlE). Maximal amount of free CA released was of 54.0 ± 1.1 to 59.8 ± 2.1 µmol/g defatted dry matter (DDM) from I-SFM (94-100% hydrolysis yield) against 42.0 ± 1.1 to 52.3 ± 0.2 µmol/g DDM (59-73% hydrolysis yield) from NI-SFM in which CA release was hampered by a phenolic oxidation side-reaction, seemingly due to meal endogenous polyphenol oxidase activities. AnFaeB and ChlE hydrolysis of phenolic extracts from NI-SFM increased the CA amount obtained to 55.0-68.1 µmol/g DDM (77-95% hydrolysis yield). In all cases, AnFaeB showed broader specificity towards SFM caffeoyl quinic acid isomers than ChlE. In particular, ChlE did not hydrolyze 3-O-caffeoylquinic acid. The maximal amount of free SA released by AnFaeA hydrolysis was 41.3 ± 0.3 µmol/g DDM from NI-SFM (50% hydrolysis yield) and 32.3 ± 0.4 µmol/g DDM from the phenolic extract (64% hydrolysis yield), with AnFaeA also having sinapine transesterification activity that led to the synthesis of 1,2-di-Osinapoyl-β-D-glucose. Finally, of all the substrates tested for enzymatic hydrolysis in our conditions, I-RSM and NI-SFM extract showed the best compromise between initial total phenolic content, hydrolysis yields and amounts of CA/SA released.

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Optimization of extraction conditions of antioxidants from sunflower shells (Helianthus annuus L.) before and after enzymatic treatment

Cited by 30 publications

References 26 publications

Optimisation of microwave‐assisted extraction of prune (Prunus domestica) antioxidants by response surface methodology

Optimisation of microwave‐assisted extraction of prune (Prunus domestica) antioxidants by response surface methodology

Antioxidant activity of phenolic compounds identified in sunflower seeds

Release of phenolic acids from sunflower and rapeseed meals using different carboxylic esters hydrolases from Aspergillus niger

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