Optimization of Extraction key Process for Erinacine from Hericium erinaceus

He, Jinzhe; Qiang, Shen

doi:10.2991/lemcs-15.2015.41

Cited by 2 publications

(2 citation statements)

References 16 publications

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“…Callus biomass that obtained through in vitro culture secondary metabolite content is influenced by the culture environment [11], extraction process and solvent used during extraction. The extraction process is bioactive material dissolved in organic solvent / ethanol [12], in glassware then excited with ultrasonic waves at a temperature of 35°C, then the extraction results are activated using a rotary vacuum evaporator at 50 o C [13].…”

Section: Introductionmentioning

confidence: 99%

Polyphenon Extraction Process From In vitro Culture of Camellia Sinensis L Callus With Ethyl Alcohol

Sutini¹,

Widiwurjani²,

Purwanto³

et al. 2018

Proceedings of the International Conference on Science and Technology (ICST 2018)

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The purpose of this study was to obtain a polyphenon profile from callus extract obtained from in vitro culture of Camellia sinensis L. Polyphenon is one of the bioactive compounds found in Camellia sinensis L plants which can also be produced through in vitro culture. Polyphenon as a bioactive is often used in various fields including in the fields of industry: manufacturing, food-drinks, health, agriculture, computers. To get a certain amount of polyphenon from in vitro culture, Camellia sinensis L callus was first extracted with water solvent, as well as non-water solvents / organic solvents such as ethyl alcohol. The methods used were: (1) inoculation of leaf explants from Camellia sinensis L in vitro on the media plus growth regulators to form callus, (2) treatment to be able to be reused (3) reap the callus followed by callus weighing and callus observation. (3) extraction and isolation of bioactive polyphenon (4) identification of polyphenon from Camellia sinensis L callus with high-performance liquid chromatography qualitatively and quantitatively. The results obtained are a form of polyphenon chromatogram profile from callus Camellia sinensis L which resembles a standard chromatogram of polyphenon.

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Section: Introductionmentioning

confidence: 99%

Polyphenon Extraction Process From In vitro Culture of Camellia Sinensis L Callus With Ethyl Alcohol

Sutini¹,

Widiwurjani²,

Purwanto³

et al. 2018

Proceedings of the International Conference on Science and Technology (ICST 2018)

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“…So, this study focuses on new sources of protein hydrolysate from monkey's head mushroom. H. erinaceus contains various bioactive constituents such as polysaccharides proteins, lectin hericenones, erinacines, sterol, fatty acid and esters (He and Shen, 2015). This study, therefore, proposes that monkey's head mushroom might be the main source of protein hydrolysate.…”

Section: Chapter I Introductionmentioning

confidence: 98%

Free radical scavenging and antiproliferative activities of peptide from monkey’s head mushroom (Hericium erinaceus)

Sangtitanu

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Free radicals have been identified as a major trigger of various syndromes in living systems. Oxidative stress occurs when free radicals are excessive and antioxidants are insufficient, resulting in the necessary consumption of additional antioxidants. Peptide derived from monkey’s head mushroom seeds was hydrolysed using various concentrations (1, 2.5, and 5%) of proteases (Alcalase, Favourzyme, and Neutrase), and the 1% Alcalase hydrolysate exhibited the highest radical scavenging activities (DPPH, ABTS, and NO assay) compared to other hydrolysates. After ultrafiltration, Mw < 0.65 kDa showed the strongest activity. Then, Mw < 0.65 kDa was purified using gel filtration chromatography and separated into two fractions (F1, and F2), of which fraction F1 exhibited the highest activity and this was further purified by reversed phase high performance liquid chromatography. Four fractions (F11, F12, F13, and F14) from RP-HPLC were isolated. Thus, four antioxidant peptides were identified by quadrupole time-of-flight (Q-TOF) mass spectrometer. The cytotoxicity activity of the fraction F1 was determined by MTT assay in five cell lines. The results showed that F1 exhibited greater inhibition against the proliferation of Chago-K1 cell lines. Moreover, the fraction F1 has the protective activity of the hydroxy radical-induced DNA damage as shown in pBR322, pKS and pUC19. The apoptosis of F1 was measured by an FITC Annexin V Apoptosis Detection Kit with PI using flow cytometry and caspase 3, 8 and 9 activities were determined in Chago-K1 cells for 24, 48, and 72 hours. The findings indicate that monkey’s head mushroom is a source of antioxidant peptides. Consequently, this suggests the importance of monkey’s head mushroom as a source of antioxidant peptides.

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Optimization of Extraction key Process for Erinacine from Hericium erinaceus

Cited by 2 publications

References 16 publications

Polyphenon Extraction Process From In vitro Culture of Camellia Sinensis L Callus With Ethyl Alcohol

Polyphenon Extraction Process From In vitro Culture of Camellia Sinensis L Callus With Ethyl Alcohol

Free radical scavenging and antiproliferative activities of peptide from monkey’s head mushroom (Hericium erinaceus)

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