Optimization of Extraction of Polyphenol from <i>Panax Quinquefolius L</i>. Using Central Composite Design/Response Surface Methodology

Zhu, Shengli

doi:10.1002/star.202100020

Cited by 4 publications

(6 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The contents of (A) fourteen saponin and (B) nine polyphenols in SLPN and SLPN-instant beverage. The extraction condition of the present study is similar to that of the other study that polyphenols are extracted from P. quinquefolius L., and the extraction rate reached 48% (34). The interaction between (A) and (C) showed that when the ethanol concentration is 30-40%, material liquid ratio 1: 3A-D).…”

Section: Saponins and Polyphenols Affect The Antioxidant Activity Of ...supporting

confidence: 83%

An instant beverage rich in nutrients and secondary metabolites manufactured from stems and leaves of Panax notoginseng

Liang

Liu²,

Li³

et al. 2022

Front. Nutr.

View full text Add to dashboard Cite

IntroductionRadix Notoginseng, one of the most famous Chinese traditional medicines, is the dried root of Panax notoginseng (Araliaceae). Stems and leaves of P. notoginseng (SLPN) are rich in secondary metabolites and nutrients, and authorized as a food resource, however, its utilization needs further research.MethodsA SLPN-instant beverage was manufactured from SLPN through optimization by response surface design with 21-fold of 48.50% ethanol for 39 h, and this extraction was repeated twice; the extraction solution was concentrated to 1/3 volume using a vacuum rotatory evaporator at 45°C, and then spray dried at 110°C. Nutritional components including 14 amino acids, ten mineral elements, 15 vitamins were detected in the SLPN-instant beverage; forty-three triterpenoid saponins, e.g., ginsenoside La, ginsenoside Rb3, notoginsenoside R1, and two flavonoid glycosides, as well as dencichine were identified by UPLC-MS.ResultsThe extraction rate of SLPN-instant beverage was 37.89 ± 0.02%. The majority nutrients were Gly (2.10 ± 0.63 mg/g), His (1.23 ± 0.07 mg/g), α-VE (18.89 ± 1.87 μg/g), β-VE (17.53 ± 1.98 μg/g), potassium (49.26 ± 2.70 mg/g), calcium (6.73 ± 0.27 mg/g). The total saponin of the SLPN-instant beverage was 403.05 ± 34.98 mg/g, majority was notoginsenoside Fd and with contents of 227 ± 2.02 mg/g. In addition, catechin and γ-aminobutyric acid were detected with levels of 24.57 ± 0.21 mg/g and 7.50 ± 1.85 mg/g, respectively. The SLPN-instant beverage showed good antioxidant activities with half maximal inhibitory concentration (IC50) for scavenging hydroxyl (OH–) radicals, superoxide anion (O2–) radicals, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS+) radicals were 0.1954, 0.2314, 0.4083, and 0.3874 mg/mL, respectively.ConclusionWe optimized an analytical method for in depth analysis of the newly authorized food resource SLPN. Together, an instant beverage with antioxidant activity, rich in nutrients and secondary metabolites, was manufactured from SLPN, which may improve the utilization of SLPN.

show abstract

Section: Saponins and Polyphenols Affect The Antioxidant Activity Of ...supporting

confidence: 83%

An instant beverage rich in nutrients and secondary metabolites manufactured from stems and leaves of Panax notoginseng

Liang

Liu²,

Li³

et al. 2022

Front. Nutr.

View full text Add to dashboard Cite

show abstract

“…At 60-70 • C, the extraction yield dropped due to the alteration of the polyphenols structure and their consequent inactivation. At 70-80 • C, the yield of polyphenols increased again, possibly due to the enhancement of some heat-sensitive polyphenols extraction [23]. Consequently, a temperature range of 50-70 • C is a suitable temperature for the PEIL extraction.…”

Section: Single-factor Experimental Analysismentioning

confidence: 99%

Optimization of Ultrasound-Assisted Extraction of Polyphenols from Ilex latifolia Using Response Surface Methodology and Evaluation of Their Antioxidant Activity

et al. 2022

View full text Add to dashboard Cite

The polyphenolic extract of Ilex latifolia (PEIL) exhibits a variety of biological activities. An evaluation of the parameters influencing the ultrasonic extraction process and the assessment of PEIL antioxidant activity are presented herein. Response surface methodology (RSM) was used to optimize the experimental conditions for the polyphenols ultrasonic-assisted extraction (UAE) from the leaves of Ilex latifolia. We identified the following optimal conditions of PEIL: ethanol concentration of 53%, extraction temperature of 60 °C, extraction time of 26 min and liquid–solid ratio of 60 mL/g. Using these parameters, the UAE had a yield of 35.77 ± 0.26 mg GAE/g, similar to the value we predicted using RSM (35.864 mg GAE/g). The antioxidant activity of PEIL was assessed in vitro, using various assays, as well as in vivo. We tested the effects of various doses of PEIL on D-galactose induced aging. Vitamin C (Vc) was used as positive control. After 21 days of administration, we measured superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities, malondialdehyde (MDA) levels in mouse serum and liver tissue. The results demonstrated that the PEIL exhibits potent radical scavenging activity against 1,1-diphenyl-2-picrythydrazyl (DPPH∙), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS+), and hydroxyl (∙OH) radicals. The serum concentrations of SOD and GSH-Px were higher, and MDA levels were lower, in the medium- and high-dose PEIL-treated groups than those in the aging group (p < 0.01), and the activity of MDA was lower than those of the model group (p < 0.01). The liver concentrations of SOD and GSH-Px were higher (p < 0.05), and MDA levels were lower, in the medium- and high-dose PEIL-treated groups than those in the aging control group (p < 0.01). These results suggest that optimizing the conditions of UAE using RSM could significantly increase the yield of PEIL extraction. PEIL possesses strong antioxidant activity and use as a medicine or functional food could be further investigated.

show abstract

“…4b show the response surface and contour of TS% and biochar. The response surface changed greatly, and the contour was irregularly circular, indicating that there was a signi cant effect between TS and biochar (Zhu et al 2021). This might be due to the signi cant effect of high-order items in the regression model (p < 0.05).…”

Section: Interaction Effects Of Ts Biochar and Fe 3 O 4 On Ad Charact...mentioning

confidence: 99%

“…(1) was obtained as follows: greatly represents the relationship between predicted and observed value(Cheng et al 2022). The lack of t P-Value of the model was 0.0625 (P > 0.05), indicating that the regression model had a high degree of t and could be used to predict the response value(Zhu et al 2021). The P-Values of different factors were less than 0.05 except x 2 , x 1 x 2 , x 1 2 x 2 , showing that they performed a signi cant impact on methane yield.…”

mentioning

confidence: 96%

Process optimization of the anaerobic co-digestion of Alternanthera philoxeroides and corn straw enhanced by biochar and Fe3O4

Fan

Shao

Guo

et al. 2022

Preprint

View full text Add to dashboard Cite

Alternanthera philoxeroides (AP), a highly invasive plant species, poses a serious threat to agriculture, ecological environment. In this study, the methane production properties of the anaerobic co-digestion (coAD) of AP and corn straw (CS) were studied. The interaction effects of total solids content (TS%), biochar, and Fe3O4 on methane yield were evaluated by regression model and response surface method (RSM). The regression model was extremely significant (p < 0.0001). The results show that the order of the effect of three factors on the methane yield of coAD was: TS% > Fe3O4 > biochar. With the aid of RSM, the optimum values of TS%, Fe3O4 and biochar were: 9.67 %, 8.41 g/kg and 5.51 %, respectively. The effects of three factors on the performance of coAD for methane yield were as follows: the content lower than the predicted optimal value was positively correlated with the methane yield, and the content higher than the predicted optimal value was negatively correlated with the methane yield. To improve the methane quality of AP, coAD could be carried out at a lower TS%, biochar and Fe3O4 were added to enhance the methane yield performance. This paper optimized the operational parameters of the coAD of AP and CS, which also provides new strategies for the treatment and utilization of AP.

show abstract

Optimization of Extraction of Polyphenol from Panax Quinquefolius L. Using Central Composite Design/Response Surface Methodology

Cited by 4 publications

References 17 publications

An instant beverage rich in nutrients and secondary metabolites manufactured from stems and leaves of Panax notoginseng

An instant beverage rich in nutrients and secondary metabolites manufactured from stems and leaves of Panax notoginseng

Optimization of Ultrasound-Assisted Extraction of Polyphenols from Ilex latifolia Using Response Surface Methodology and Evaluation of Their Antioxidant Activity

Process optimization of the anaerobic co-digestion of Alternanthera philoxeroides and corn straw enhanced by biochar and Fe3O4

Contact Info

Product

Resources

About