Optimization of Fermentation Conditions for Antibiotic Production by Actinomycetes YJ1 Strain against Sclerotinia sclerotiorum

Abstract

In order to improve the productivity of activities substance of actinomycetes YJ1, the effects of the medium components, inoculation volume, medium capacity, rotary speed, fermentation time, temperature and initial pH on the biological activity of YJ1 were investigated by detecting the mycelium growth inhibition rate of Sclerotinia sclerotiorum. The results show that the inhibition rate is the highest under the optimum medium composition (g/L), containing sucrose 10.0, soluble starch 10.0, soybean meal 20.0, K 2 HPO 4 0.5, MgSO 4 0.5, NaCl 1.0 and FeSO 4 0.01. The maximum antibiotic activity is obtained at the inoculation volume 5%, medium volume in 250 ml flask 75 ml, rotary speed of 180 r/min, fermentation time 4 days, temperature 28°C and initial pH 7.0. The inhibition rate has been increased by 6.71% under the optimized condition.

Optimization of Nutrient and Fermentation Parameters for Antifungal Activity byStreptomyces lavendulaeXjy and Its Biocontrol Efficacies againstFulvia fulvaandBotryosphaeria dothidea

“…; Song et al. ). In this study, both fermentation conditions and medium components were optimized by RSM methods to effectively enhance the antifungal activities of S. lavendulae Xjy.…”

“…(P = 0.05). get maximum yield of antimicrobial metabolites (Ripa et al 2009;Kiranmayi et al 2011;Song et al 2012). In this study, both fermentation conditions and medium components were optimized by RSM methods to effectively enhance the antifungal activities of S. lavendulae Xjy.…”

Metabolic analysis reveals the amino acid responses of Streptomyces lydicus to pitching ratios during improving streptolydigin production

“…It was reported that the high cell density fermentations could be achieved by improving the oxygen conditions without affecting drastically the cellular physiological conditions (Verbelen et al 2009). However, in Actinomycetes YJ1 strain, the maximum antibiotic activity against Sclerotinia sclerotiorum was obtained at the inoculation volume of 5 %, while the antibiotic activity was not enhanced with the increases of pitching ratio (Song et al 2012). It was also demonstrated that the high pitching ratio (30 %, v/v) significantly enhanced the streptolydigin production of S. lydicus E9 (Cheng et al 2009).…”

Screening, identification, optimization of fermentation conditions, and extraction of secondary metabolites for the biocontrol of Rhizoctonia Solani AG-3

“…Several actinomycetes require high amount of oxygen during the process of fermentation. Cultural medium contained organic and inorganic elements that lead to decrease in the level of dissolved oxygen; to meet this requirement there should be proper ventilation [26]. Temperature had great impact on production, during low temperature environmental pressure affected the secondary metabolites and resulted in amplified production [27].…”

“…The temperature affects the growth of actinomycetes. Favourable growth temperature is [23][24][25][26][27][28][29][30][31][32][33][34][35][36][37] C. Growth of the bacteria affected by the temperature and aeration that resulted influenced on the production of the antibiotics [28,29]. Another important factor that influences the production of the antibiotic is inoculum volume.…”