Optimization of fibrinolytic protease production from Bacillus subtilis I-2 using agro-residues

Bajaj, Bijender Kumar; Singh, Satbir; Khullar, Mowkshi; Singh, K. C.; Bhardwaj, Shikha

doi:10.1590/s1516-8913201402132

Cited by 49 publications

(35 citation statements)

References 26 publications

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“…This is a likely pointer towards the role, though minor in magnitude, played by the carbon source in enhancing the FEA in the media. In our study, we found that a nitrogen source played a major role played in enhancing the fibrinolytic activity in the media and was in good agreement with the results reported by Bajaj et al (2014). We determined that there is no appreciable change in activity as the inoculum increases from 1% (v/v) to 5% (v/v) and a further increase to 7% (v/v) inoculum volume led to reduced activity levels.…”

Section: Discussionsupporting

confidence: 91%

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Fibrinolytic enzyme production by newly isolated Bacillus cereus SRM-001 with enhanced in-vitro blood clot lysis potential

Narasimhan

Chandrasekaran²,

Rajesh

2015

J. Gen. Appl. Microbiol.

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The discovery of plasmin-like microbial fibrinolytic enzymes having high specificity and negligible side effects is crucial for thrombolytic therapy. Herein, we report one such extra-cellular fibrinolytic enzyme producing Bacillus cereus SRM-001 isolated from the blood-laden soil of a chicken dump yard. The potency of the enzyme was established with fibrin plate assay and in-vitro blood clot lysis assay. The shake-flask operating parameters and media composition were optimized for maximizing the productivity of the enzyme. The operating parameters, pH 7, 37°C, 1% inoculum volume and 24 h inoculum age, were found to be the optimum. The levels of media components, corn flour (0.3% w/v), soyabean powder (1.9% w/v) and MnSO 4 (11.5 mM) were optimized by statistical analysis using Box-Behnken design derived RSM. This resulted in an almost 1.8 fold increase in fibrinolytic enzyme productivity. The 3D response surface plots showed soyabean powder and MnSO 4 to be the key ingredients for enhancing the enzyme productivity, whereas corn flour had a marginal effect. The in-vitro blood clot lysis assay conducted at near physiological pH 7 at 37°C showed the enzyme to be a potential therapeutic thrombolytic agent.

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Section: Discussionsupporting

confidence: 91%

“…Microbial production of plasmin-like protease from microorganisms such as Bacillus sp. (Afifah et al, 2014;Bajaj et al, 2014;Gu et al, 2009;Liu et al, 2005) and Petasites japonicas (Kim et al, 2015), etc., have been reported in the literature.…”

Section: Introductionmentioning

confidence: 99%

Fibrinolytic enzyme production by newly isolated Bacillus cereus SRM-001 with enhanced in-vitro blood clot lysis potential

Narasimhan

Chandrasekaran²,

Rajesh

2015

J. Gen. Appl. Microbiol.

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“…Fibrin plays a vital role in health and healing; however, formation of inappropriate clot especially under certain pathophysiological conditions in the body is a major risk factor for heart disorders (Bajaj et al , 2014. Homoeostasis of formation and dissolution of fibrin maintain appropriate viscosity in the vascular system.…”

Section: Fibrinolytic Proteases As Thrombolytic Agentsmentioning

confidence: 99%

“…Enzyme-based industrial processes are much more environmentally safer than the traditional chemical ones and involve less emissions (Sheldon et al 2007). But considering that enzymes are the biological molecules which are largely designed (by nature) to function under ambient conditions in the living systems, may not suit well for relatively hostile industrial process conditions (Bajaj et al 2014). Therefore, discovery of sturdy enzymes that are capable of functioning under industrial processes has gained considerable research impetus in recent years (Sharma and Bajaj 2017).…”

Section: Introductionmentioning

confidence: 99%

Potential application spectrum of microbial proteases for clean and green industrial production

Singh

Bajaj

2017

Energ. Ecol. Environ.

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Enzymes are the cornerstones of metabolism and constitute the fundamental basis for existence of life. However, recently enzymes are being implicated in diverse industrial processes because of their specific and fast action for efficient bioconversion of substrate to product, and their capability to save raw materials, energy and chemicals for various manufacturing processes. Enzymes are considered as environment-friendly (green) chemicals that may potentially help replacing completely or reducing the usage of hazardous chemicals for industrial processes, thus promising sustainable production and manufacturing. Among various industrial enzymes microbial proteases dominate the world enzyme market due to their multifaceted application potential in varied bioindustries like food, pharmaceutical, textile, photographic, leather and detergent. Promising applications of proteases in agricultural sector for instance may include biocontrol of pests, degumming of silk, selective delignification of hemp and wool processing. However, for successful industrial applications the proteases must be robust enough to suit the process conditions which are generally hostile. Proteases intended for industrial applications must have activity and stability over wide range of temperature and pH extremes for prolonged time periods and even in the presence of various potential enzyme inhibitors. Of various microbial proteases those from Bacillus spp. have got special significance because the latter are known for their ability to produce sturdy enzymes that might have suitability for industrial process conditions. The current article presents an interpretive summary of the recent developments on application potential of proteases for various industries.

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“…Lately, many studies have applied this technique for the production of industrially important enzymes such as cyanide degradation ability of bacterial consortium was enhanced by the statistical methods of optimization [13]. Similarly, culture conditions have been optimized for enhanced production of protease from Bacillus subtilis [14]. RSM being less laborious and timeconsuming has been applied successfully in biotechnological processes to decreases the number of experimental trials [15].…”

Section: Introductionmentioning

confidence: 99%

Statistical Optimization of Production Conditions of Alkaline Pectin Lyase from Bacillus cereus using Response Surface Methodology

Reena¹,

Pooja²,

Nitish³

2016

J Food Ind Microbiol

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Pectin lyase finds applications in the degumming and retting of plant fibers where it degrades highly methylesterified pectin without prior action of any other pectinase. Response surface methodology (RSM) has been frequently utilized for the optimization of production process of industrially important enzymes from microbes. In the present work, fermentation conditions for the production of pectin lyase from Bacillus cereus were optimized using the factorial and central composite design of RSM. The cubic order polynomial regression model was found to be adequate and significant with a determination coefficient R 2 of 0.9505 (p<0.0001). The ANOVA analysis and three dimensional surface plots confirmed interaction among variables. The optimum values of variables were found to be pectin 4% (w/v), sodium carbonate 1% (w/v), manganese sulfate 0.055% (w/v) and magnesium sulfate 0.018% (w/v) at 150 rpm under response surface curves. After optimization, the experimental value of maximum activity of enzyme (3.37 U/ml) was found higher but close to the predicted value (2.68 U/ml) and the enzyme activity increased by 1.32 fold (32.16%).

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Optimization of fibrinolytic protease production from Bacillus subtilis I-2 using agro-residues

Abstract: ABSTRACT

Cited by 49 publications

References 26 publications

Fibrinolytic enzyme production by newly isolated <i>Bacillus cereus</i> SRM-001 with enhanced <i>in-vitro</i> blood clot lysis potential

Fibrinolytic enzyme production by newly isolated <i>Bacillus cereus</i> SRM-001 with enhanced <i>in-vitro</i> blood clot lysis potential

Potential application spectrum of microbial proteases for clean and green industrial production

Statistical Optimization of Production Conditions of Alkaline Pectin Lyase from Bacillus cereus using Response Surface Methodology

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