Abstract-The performance of the Proton Exchange Membrane fuel cell (PEMFC) depends on operating and design parameters. In this study, the effect of various pressure, temperature, inlet stoichiometric ratio of reactant mass flow rate and various landing to channel width ratio of (L:C) 1:1, 1:2, 2:1 and 2:2 with serpentine flow channel of 25cm 2 active area of the PEMFC has been considered. The optimum power density of the PEMFC obtained by optimizing these parameters using the Response Surface Methodology (RSM) has been considered. The numerical power densities of serpentine flow channel with L:C -1:1, 1:2, 2:1 and 2:2 were found to be 0.308, 0.321, 0.304 and 0.312 W /cm 2 by the analysis of variance (ANOVA) using Minitab 17 and obtained combinations of parameters were fed into computational fluid dynamics (CFD) Fluent software and the corresponding experimental power density was to be 0.250, 0.260, 0.259 and 0.260 W/cm 2 respectively. It was found that the numerical studies on serpentine flow field results has been deviated from the experimental results for various L:C of 1:1, 1:2, 2:1 and 2:2 as 19.2 %, 16.5 % ,16.2 % and 11.9 % respectively. Keyword -Response Surface Methodology, computational fluid dynamics, serpentine flow channel, landing to channel width ratio, PEMFC.I. INTRODUCTION The PEMFC engines can potentially interchange the internal combustion (IC) engine for transportation because it is clean, quiet, energy efficient, modular and capable of quick start-up [1]. The PEMFCs are considered to be appropriate solution to environmental and energy problems and are expected to soon become the most promising energy converters for automotive, stationary and portable applications, because of their high-energy density at low operating temperatures, quick start-up and zero emissions [2].The performance of the PEMFC depends on the various design and operating parameters revealed from the various literature survey. The performance of PEMFC with the active area of 5 cm 2 under various operating conditions (pressures, temperatures and humidity of reactant gases) was investigated experimentally by Amirinejad et al. [3].The results revealed that the performance of PEMFC increased with the increase of the cell temperature and operating pressure. The performance of the cell, not only depends on the reactants humidification, but the anode humidification has also a considerable effect on the fuel cell performance. The various operating parameters like cell operating pressure, temperature, and reactants on anode and cathode flow rate has been investigated experimentally for triangular channel geometry on 25 cm 2 active area of PEMFC by Khazaee et al. [4].The results showed that the PEMFC performance can be enhanced by increase in the inlet temperature of reactants, cell temperature and inlet pressure. Santarelli & Torchio [5] discussed the characterization of the behavior of a PEMFC effective area of 25 cm 2 with various operating parameters (cell temperature, the anode and the cathode flow temperature in saturation and ...