Optimization of growth regulators to induce callus in chili [Capsicum annuum] cv. Berangkai

Yunita, Rossa; Bagus, F R M; Nova, Bastian; Rosadi, Firsta Ninda; Jamsari, J

doi:10.1088/1755-1315/741/1/012047

Cited by 4 publications

(4 citation statements)

References 10 publications

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“…[20,21] Leaves They have an entire (unlobed) or palmatifid (divided/lobed) shape which ranges from 10 to 15 cm long with filiform stipules, 5-8 mm long pubescent, and a petiole which is about 3-30 cm long. [20,23,[28][29][30] Pedicel Articulated at the base; the length of the structure ranges from 2 to 6 mm.…”

Section: Laminamentioning

confidence: 99%

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Cultivation Strategies of Kenaf (Hibiscus cannabinus L.) as a Future Approach in Malaysian Agriculture Industry

et al. 2023

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In recent years, kenaf has gained significant global attention as a more cost-effective, adaptable, and manageable alternative to other fibre crops. India and China, with nearly 70% of the global kenaf production, have emerged as the leading producers of kenaf plants. While kenaf was traditionally valued for its paper production, it has evolved into a multipurpose crop with diverse industrial applications over the past two decades. Conventional soil-based cultivation methods for kenaf require up to six months for plant maturity. However, in vitro propagation techniques offer a promising alternative that enables faster growth and reduced labour costs. In vitro propagation can be achieved using solid and liquid media, with limited research available on the pure liquid culture method for kenaf. This review aims to introduce and compare the production of kenaf using solid and liquid media, with a specific focus on the emerging country of Malaysia, which seeks to harness the potential of kenaf cultivation for the 15th Sustainable Development Goal, “life on land”, and its contribution to the economy.

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Section: Laminamentioning

confidence: 99%

“…[ [28][29][30]32] In addition to its many practical applications, kenaf is also a highly sustainable crop. It requires less water and pesticides than other crops and can be grown in areas that are not suitable for other types of agriculture.…”

Section: Stemmentioning

confidence: 99%

Cultivation Strategies of Kenaf (Hibiscus cannabinus L.) as a Future Approach in Malaysian Agriculture Industry

et al. 2023

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“…The first indication of callus appearance is an enlargement of the callus, and then callus forms on the explant's cut end [10]. The callus is formed by cutting explants in contact with the medium supplemented with appropriate growth regulators [11]. The fastest treatment to induce Calotropis gigantea callus is BAP 0,5 ppm + 2,4-D 1,5 ppm, 6 days after induction.…”

Section: Callus Formation Timementioning

confidence: 99%

Callus induction of Calotropis gigantea using BAP and 2,4-D in vitro

Muthiah

Sakya

Setyawati

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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Calotropis gigantea is a plant not widely used but has potential as a medicinal plant. The phytochemical compound can be produced faster using a tissue culture and a combination of growth regulators. The research objectives were to study the effect and determine the best concentration of BAP and 2,4-D in callus induction of Calotropis gigantea. This research was carried out in the Plant Physiology and Biotechnology Laboratory, Faculty of Agriculture, Sebelas Maret University, from September to November 2021. A month-old Calotropis gigantea stem was used as an explant. The research used three replicates of a completely randomized design with 25 combination treatments. The culture medium was MS with BAP (0; 0.5; 1; 1.5; 2 ppm) and 2,4-D (0; 0.5; 1; 1.5; 2 ppm) supplements. Parameters observed in this research were callus formation time, callus colour, and callus texture. The results showed that a combination of BAP 0.5 ppm + 2,4-D 1.5 ppm could induce callus faster than other treatments, 6 days. Callus morphology with green color and compact texture resulted from a combination of BAP 1.5 ppm + 2,4-D 1.5 ppm treatment.

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“…Chili has less ability to regenerate while other Solanaceae crops such as tomato, tobacco, potato are frequently used as model systems due to their ability to regenerate plants. Regardless of the fact many findings had been conducted for the relative success of the regeneration system in different crops (Kim et al, 2008;Keshavareddy et al, 2018) on shoot morphogenesis in chili but genetic engineering is still restricted by the low morphogenetic potential of Sarhad Journal of Agriculture these species (Mihálka, 2006;Yunita et al, 2021). Explant selection at a given stage of the plant, as well as changes in nutritional media and additives, could all aid to reduce recalcitrance (Kothari et al, 2010;Bridgen et al, 2018).…”

Section: Effect Of Different Factors On Agrobacterium-mediated Plant ...mentioning

confidence: 99%

Response of Different (Capsicum annuum L) Genotypes for Callus Induction, Plant Regeneration and Plant Transformation

Shafiq¹,

Ashraf²,

Mushtaq³

et al. 2022

SJA

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C hili peppers are members of the Capsicum genus, which belongs to the family Solanaceae, ae (Kothari et al., 2010;Jeong et al., 2011). There are 5 cultivated and 26 wild Capsicum species in the genus Capsicum (Wahyuni et al., 2013). Chili is an important cash crop in Pakistan, with a total cultivated area Abstract | Chilli (Capsicum annuum), which belongs to the family Solanaceae, is an important spice crop cultivated in tropical and subtropical countries. A Protocol for chili plant regeneration with hypocotyl and cotyledon explants was established. The study was conducted to observe the effect of genotypes, culture conditions and growth regulators on plant regeneration of chili pepper genotypes (Seedex Pepper (SP), Loungi, Tatapuri, and Sanam) grown in Pakistan including. For both hypocotyl and cotyledon explants, SP was found to be the most sensitive among the tested genotypes tested. Maximum mean callus induction rate of hypocotyl was 76.6% in SP followed by TP (73.3%); Sanam (57.3%); Loungi (43.3%) respectively. Whereas in case of cotyledon maximum inductions rate was found in Loungi (62.0%) followed by SP (60); Sanam (57.3); TP (46) individually. According to the observations maximum plant regeneration was found in genotype SP (9%), followed by Loungi (1.8%); Sanam (1.5%) but no plant regeneration was found in TP. A. tumefaciens LBA4404 with the 35S GFP/pFGC construct were used to evaluate hypocotyl and cotyledon explants for transformation. The effects of co-cultivation at different temperatures (22 and 25 o C), photoperiods (16 hours of light, 8 hours of darkness, and full darkness), and co-cultivation periods were studied. GFP assays revealed that the putative transgenic calli were not transformed and died after 40-60 days. The experiment was repeated ten times to see the success rate of the regeneration system. In this study a protocol for chili plant regeneration was developed for callus induction, plant regeneration, and plant transformation. Because developing a viable chilli tissue culture and plant regeneration system is difficult, this study was designed to help develop one. SP was found to be the most suitable among the four genotypes used for regeneration.

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Optimization of growth regulators to induce callus in chili [Capsicum annuum] cv. Berangkai

Cited by 4 publications

References 10 publications

Cultivation Strategies of Kenaf (Hibiscus cannabinus L.) as a Future Approach in Malaysian Agriculture Industry

Cultivation Strategies of Kenaf (Hibiscus cannabinus L.) as a Future Approach in Malaysian Agriculture Industry

Callus induction of Calotropis gigantea using BAP and 2,4-D in vitro

Response of Different (Capsicum annuum L) Genotypes for Callus Induction, Plant Regeneration and Plant Transformation

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