Optimization of in vitro refolding conditions of recombinant Lepidium draba peroxidase using design of experiments

Fattahian, Yaser; Riahi‐Madvar, Ali; Mirzaee, Reza; Torkzadeh‐Mahani, Masoud; Asadikaram, Gholamreza; Sargazi, Ghasem

doi:10.1016/j.ijbiomac.2018.06.122

Cited by 12 publications

(5 citation statements)

References 32 publications

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“…The tRNA genes of the engineering strain E. coli Rosetta include rare codons AGG, AGA, AUA, CUA, CCC, and GGA. Therefore, codon usage optimization is critical in E. coli [42,43].…”

Section: Expression and Purification Of The Recombinant Fluorinasementioning

confidence: 99%

Facile Bioinspired Preparation of Fluorinase@Fluoridated Hydroxyapatite Nanoflowers for the Biosynthesis of 5′-Fluorodeoxy Adenosine

Wang

et al. 2020

Sustainability

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To develop an environmentally friendly biocatalyst for the efficient synthesis of organofluorine compounds, we prepared the enzyme@fluoridated hydroxyapatite nanoflowers (FHAp-NFs) using fluorinase expressed in Escherichia coli Rosetta (DE3) as the biomineralization framework. The obtained fluorinase@FHAp-NFs were characterized by scanning electron microscope (SEM), X-ray diffraction (XRD), and FT-IR spectrum and used in the enzymatic synthesis of 5′-fluorodeoxy adenosin with S-adenosyl-L-methionine and fluoride as substrate. At an optimum pH of 7.5, fluorinase confined in the hybrid nanoflowers presents an approximately 2-fold higher synthetic activity than free fluorinase. Additionally, after heating at 30 °C for 8 h, the FHAp-NFs retained approximately 80.0% of the initial activity. However, free enzyme could remain only 48.2% of its initial activity. The results indicate that the fluoride and hybrid nanoflowers efficiently enhance the catalytic activity and thermal stability of fluorinase in the synthesis of 5′-fluorodeoxy adenosine, which gives a green method for producing the fluorinated organic compounds.

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“…The tRNA genes of the engineering strain E. coli Rosetta include rare codons AGG, AGA, AUA, CUA, CCC, and GGA. Therefore, codon usage optimization is critical in E. coli [42,43].…”

Section: Expression and Purification Of The Recombinant Fluorinasementioning

confidence: 99%

Facile Bioinspired Preparation of Fluorinase@Fluoridated Hydroxyapatite Nanoflowers for the Biosynthesis of 5′-Fluorodeoxy Adenosine

Wang

et al. 2020

Sustainability

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“…In one such case study, this strategy delivered a 48% refolding yield for Lepidium draba peroxidase. 29 Both the techniques, DOE and ML, are mathematical approaches, and many researchers have worked on the intersection of these two fields. DOE has been used to determine initial parameter tuning for the SVR algorithm 30 and also to initialize NN instead of employing a hit and trial method for the same.…”

Section: Introductionmentioning

confidence: 99%

“…In one such case study, this strategy delivered a 48% refolding yield for Lepidium draba peroxidase. 29…”

Section: Introductionmentioning

confidence: 99%

Process optimization using machine learning enhanced design of experiments (DOE): ranibizumab refolding as a case study

et al. 2023

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“…Nanotechnology has many remarkable applications in different industries. − The nanodrug carrier systems deliver therapeutic agents because of their ability and enhanced efficiency to encapsulate both hydrophilic and hydrophobic drug molecules and can also be used in bioimaging applications. , Today, nanotechnology-based diagnostic assays and therapeutic procedures fall within the scope of a broader field known as nanomedicine, which is heavily dependent on developing novel nanocarriers. − In addition to diagnosis and monitoring, nanotechnology has also contributed to developing novel therapies for a variety of diseases and cancers. − These nanocarriers protect the encapsulated drugs from physiological degradation, which assist with solubility, and ensure the sustained/controlled release of the drug from the carrier with a precise delivery rate and dose regimen to increase its bioavailability level . Among novel noncarriers, niosome gained a lot of attention in recent years.…”

Section: Introductionmentioning

confidence: 99%

Preparation of pH-Responsive Vesicular Deferasirox: Evidence from In Silico, In Vitro, and In Vivo Evaluations

et al. 2021

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pH-sensitive nanocarriers can effectively deliver anticancer drugs to tumors and reduce the adverse effects of conventional chemotherapy. In this light, we prepared a novel pH-responsive deferasirox (DFX)-loaded vesicle and comprehensively performed in silico, in vitro, and in vivo studies to examine the properties of the newly synthesized formulation. Physiochemical assessment of the developed formulations showed that they have an average size (107 ± 2 nm), negative zeta potential (−29.1 ± 1.5 mV), high encapsulation efficiency (84.2 ± 2.6%), and a pH-responsive release. Using the molecular dynamics simulation, the structural and dynamic properties of ergosterol-containing niosomes (ST60/Ergo) in the presence of DFX molecules were analyzed and showed a good interaction between DFX and vesicle components. Cytotoxic assessment showed that niosomal DFX exhibited a greater cytotoxic effect than free DFX in both human cancer cells (MCF-breast cancer and Hela cervical cancer) and induced evident morphological features of apoptotic cell death. No marked difference between the ability of free and niosomal DFX was found in activating caspase-3 in Hela cells. Eight weeks of intraperitoneal administrations of free DFX at three doses caused a significant increase in serum biochemical parameters and liver lipid peroxidation. Treatment with 5 mg/kg dose of niosomal DFX caused a significant increase in serum creatinine (P < 0.05); however, other parameters remained unchanged. On the other hand, administration of niosomal DFX at the highest dose (10 mg/kg) significantly increased serum creatinine (P < 0.05), BUN, and serum liver enzymes compared to the control rats (P < 0.001). Based on the results, the application of pH-responsive DFX-loaded niosomes, as a novel drug delivery platform, may yield promising results in cancer treatment.

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Optimization of in vitro refolding conditions of recombinant Lepidium draba peroxidase using design of experiments

Cited by 12 publications

References 32 publications

Facile Bioinspired Preparation of Fluorinase@Fluoridated Hydroxyapatite Nanoflowers for the Biosynthesis of 5′-Fluorodeoxy Adenosine

Facile Bioinspired Preparation of Fluorinase@Fluoridated Hydroxyapatite Nanoflowers for the Biosynthesis of 5′-Fluorodeoxy Adenosine

Process optimization using machine learning enhanced design of experiments (DOE): ranibizumab refolding as a case study

Preparation of pH-Responsive Vesicular Deferasirox: Evidence from In Silico, In Vitro, and In Vivo Evaluations

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