Optimization of Irreversible Electroporation Protocols for In-vivo Myocardial Decellularization

Zager, Yaniv; Kain, David; Landa, Natalie; Leor, Jonathan; Maor, Elad

doi:10.1371/journal.pone.0165475

Cited by 59 publications

(54 citation statements)

References 20 publications

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“…notably compared different protocols, observing that longer pulse duration, multiple pulses, higher current voltage, and lower interpulse distance were associated with bigger tissue damage. They also observed a key feature of IRE, which resides in sparing the extracellular structures …”

Section: Application Of Ire In Cardiologymentioning

confidence: 98%

“…Furthermore, safety issues concerning the formation of gas bubbles during applications must be considered, due to the long duration of defibrillation pulses (6–10 ms). IRE DC‐PEFs can be generated also by benchmark programmable electroporators for molecular biology, such as BTX ECM 830 (Harvard Apparatus) square wave pulse generator . Commercial dedicated IRE DC pulse generator is for now represented only by the Nanoknife ® system (HVP‐01 Electroporation System, Angiodynamics).…”

Section: Application Of Ire In Cardiologymentioning

confidence: 99%

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Irreversible electroporation ablation for atrial fibrillation

Wojtaszczyk

Caluori

Pešl

et al. 2018

Cardiovasc electrophysiol

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Atrial fibrillation (AF) is one of the most important problems in modern cardiology. Thermal ablation therapies, especially radiofrequency ablation (RF), are currently "gold standard" to treat symptomatic AF by localized tissue necrosis. Despite the improvements in reestablishing sinus rhythm using available methods, both success rate and safety are limited by the thermal nature of procedures. Thus, while keeping the technique in clinical practice, safer and more versatile methods of removing abnormal tissue are being investigated. This review focuses on irreversible electroporation (IRE), a nonthermal ablation method, which is based on the unrecoverable permeabilization of cell membranes caused by short pulses of high voltage/current. While still in its preclinical steps for what concerns interventional cardiac electrophysiology, multiple studies have shown the efficacy of this method on animal models. The observed remodeling process shows this technique as tissue specific, triggering apoptosis rather than necrosis, and safer for the structures adjacent the myocardium. So far, proposed IRE methodologies are heterogeneous. The number of devices (both generators and applicators), techniques, and therapeutic goals impair the comparability of performed studies. More questions regarding systemic safety and optimal processes for AF treatment remain to be answered. This work provides an overview of the electroporation process, and presents different results obtained by cardiology-oriented research groups that employ IRE ablation, with focus of AF-related targets. This contribution on the topic aspires to be a practical guide to approach IRE ablation for cardiac arrhythmias, and to highlight controversial features and existing knowledge, to provide background for future improved experimentation with IRE in arrhythmology.

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Section: Application Of Ire In Cardiologymentioning

confidence: 98%

Section: Application Of Ire In Cardiologymentioning

confidence: 99%

Irreversible electroporation ablation for atrial fibrillation

Wojtaszczyk

Caluori

Pešl

et al. 2018

Cardiovasc electrophysiol

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“…Hypotonic treatment has been reported to cause minimal ECM damage . Other physical techniques which have also been reported to assist in cell lysis include sonication and electroporation which exert their effect by disrupting cell membranes …”

Section: Ecm Decellularization Techniquesmentioning

confidence: 99%

Processed Tissue–Derived Extracellular Matrices: Tailored Platforms Empowering Diverse Therapeutic Applications

Krishtul

Baruch

Machluf

2019

Adv Funct Materials

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Tissue-derived decellularized extracellular matrices (dECM) have gradually become the gold standard of scaffolds for tissue engineering, owing to their close mirroring of the intricate composition, architecture, and topology of the native extracellular matrix (ECM). Intriguingly, further manipulation of these acellular tissues through various processing techniques has been demonstrated to be an effective strategy to control their characteristics and impart them with ample valuable new traits, thereby expanding their applicability to a significantly wider spectrum of research and translational applications. Herein, state-of-the-art processed dECM platforms and their potential applications are focused on. The ECM characteristics that make it so appealing for tissue engineering are presented, followed by a concise discussion on the main considerations for choosing a dECM source for such applications. The key methodologies for dECM processing, including hydrogel production, bioprinting, electrospinning, and production of porous scaffolds, microcarriers, and microcapsules, as well as their inherent advantages and challenges, are introduced. To demonstrate the use of processed dECM platforms for tissue engineering, selected in vivo and in vitro applications recently developed utilizing these platforms are highlighted. Finally, concluding remarks and a prospective outlook for future developments and improvements in the field of processed dECM-based devices are given.

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“…Zager [85] meticulously evaluated for a graded effect of different electroporation protocols on in vivo myocardial tissue. Results from their study showed IRE has a graded effect on the myocardium and that the extent of ablation can be controlled by changing pulse length, frequency and number, as well as by changing electric field intensity.…”

Section: Cardiac Tissuementioning

confidence: 99%

Irreversible electroporation for the treatment of cardiac arrhythmias

Sugrue

Maor

Ivorra

et al. 2018

Expert Review of Cardiovascular Therapy

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Cardiac ablation is an established treatment modality for the management of patients with cardiac arrhythmias. Current approaches to cardiac ablation employ thermal based energy to achieve lesions (damage) within the heart. There are many shortcomings and limitations of thermal based approaches. Electroporation (DC energy) is a non-thermal alternative approach to ablation that has shown significant promise in animal studies. Areas covered: An extensive review of the literature on the application of electroporation for ablation (both cardiac and collateral cardiac tissue) was undertaken. This review explores irreversible electroporation as a cardiac ablation modality. Specifically, it focuses and explains the biophysics of electroporation, the limitations of current thermal based approaches and examines the current data published on electroporation cardiac ablation. Expert commentary: Electroporation is a fast-growing novel ablation modality that has many advantages over current thermal based approaches. Current research in animal models shows its can be safely and efficaciously applied to the heart. Although further research is required, electroporation represents an appealing option for the ablation cardiac arrhythmias.

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Optimization of Irreversible Electroporation Protocols for In-vivo Myocardial Decellularization

Cited by 59 publications

References 20 publications

Irreversible electroporation ablation for atrial fibrillation

Irreversible electroporation ablation for atrial fibrillation

Processed Tissue–Derived Extracellular Matrices: Tailored Platforms Empowering Diverse Therapeutic Applications

Irreversible electroporation for the treatment of cardiac arrhythmias

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