Optimization of liquid chromatographic method for the separation of nine hydrophilic and hydrophobic components in Salviae miltiorrhizae Radix et Rhizoma (Danshen) using microemulsion as eluent

Huang, Hongzhang; Xuan, Xueyi; Xu, Liyuan; Yang, Jianrui; Gao, Chen; Li, Ning

doi:10.1016/j.jchromb.2014.02.027

Cited by 11 publications

(8 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…C. However, an attempt to acquire a better resolution by increasing the pH level to 9.0 was not successful and led to a worse separation, especially for compounds 3–7 (pKa = 2.77–5.47) , as depicted in Fig. D and E, indicating that these separations were very sensitive to pH changes.…”

Section: Resultsmentioning

confidence: 99%

Surfactant‐coated graphitized multiwalled carbon nanotubes as the pseudostationary phase in electrokinetic chromatography for the analysis of phytochemical compounds in biological fluids

Cao

Ying

et al. 2015

Electrophoresis

View full text Add to dashboard Cite

This report describes the use of surfactant-coated graphitized multiwalled carbon nanotubes (SC-GMWNTs) as a novel pseudostationary phase in CE with diode array detection for the determination of phenolic acids and tanshinones in herbal and urine samples. Several parameters influencing the separation were studied, such as the concentrations of SDS, GMWNTs, and isopropanol; choice of carbon nanotubes; sodium borate content; and buffer pH. The results revealed that the presence of SC-GMWNTs in buffer enhanced the separation efficiency for the target analytes relative to conventional micelles due to the strong interaction between the surface of the GMWNTs and the target compounds. Under the optimum conditions, the method showed good linearity, with correlation coefficients higher than 0.9950. LODs were in the range of 0.71-3.10 μg/mL. Furthermore, satisfactory separations were achieved with good recovery values in the range of 89.97 and 103.30% when 10 mM borate, 30 mM SDS, 10% isopropanol, and 6 μg/mL SC-GMWNTs were introduced into the buffer solution.

show abstract

Section: Resultsmentioning

confidence: 99%

Surfactant‐coated graphitized multiwalled carbon nanotubes as the pseudostationary phase in electrokinetic chromatography for the analysis of phytochemical compounds in biological fluids

Cao

Ying

et al. 2015

Electrophoresis

View full text Add to dashboard Cite

show abstract

“…In a previous article (Huang et al ., ), 26 microemulsion systems were investigated for the optimization of chromatographic conditions (Table ) according to the influence of different column types and compositions (surfactant, oil, pH and additive). The optimal mobile phase was identified by central composite design and response surface methodology, and used to separate the components of danshen root.…”

Section: Resultsmentioning

confidence: 99%

Determination of the lipophilicity of Salvia miltiorrhiza Radix et Rhizoma (danshen root) ingredients by microemulsion liquid chromatography: optimization using cluster analysis and a linear solvation energy relationship‐based method

Yang

Huang

et al. 2015

Biomedical Chromatography

Self Cite

View full text Add to dashboard Cite

We evaluated 26 microemulsion liquid chromatography (MELC) systems for their potential as high-throughput screening platforms capable of modeling the partitioning behaviors of drug compounds in an n-octanol-water system, and for predicting the lipophilicity of those compounds (i.e. logP values). The MELC systems were compared by cluster analysis and a linear solvation energy relationship (LSER)-based method, and the optimal system was identified by comparing their Euclidean distances with the LSER coefficients. The most effective MELC system had a mobile phase consisting of 6.0% (w/w) Brij35 (a detergent), 6.6% (w/w) butanol, 0.8% (w/w) cyclohexane, 86.6% (w/w) buffer solution and 8 mm cetyltrimethyl ammonium bromide. The reliability of the established platform was confirmed by the agreement between the experimental data and the predicted values. The logP values of the ingredients of danshen root (Salvia miltiorrhiza Radix et Rhizoma) were then predicted. Copyright © 2015 John Wiley & Sons, Ltd.

show abstract

“…During the analyses, the column temperature was set at 25 °C, and the injection volume was 10 μL. The flow rate of the mobile phase was controlled at 1.0 mL/min and peaks were detected at 210 nm 21,22 .…”

Section: Methodsmentioning

confidence: 99%

“…When the chromophore was limited, the detection was superior to conventional HPLC 16 . MELC has been successfully applied to determine drugs in pharmaceutical dosage form 17,18 , to analyze biological samples 19,20 , and to separate and determine content in Chinese traditional medicine 21,22 .…”

Section: Introductionmentioning

confidence: 99%

A microemulsion high-performance liquid chromatography (MELC) method for the separation and determination of hydrolyzed tenuifolin in Radix Polygalae

Yan

Zheng

et al. 2019

Sci Rep

View full text Add to dashboard Cite

Tenuifolin was used as a reliable chemical marker for the quality control of Radix Polygalae. The determination of tenuifolin is challenging because the analyte molecule lacks a suitable chromophore. The aim of this study was to establish a microemulsion high-performance liquid chromatography (MELC) method which is robust and sensitive, and can separate and determine tenuifolin in Radix Polygalae using an oil-in-water (O/W) microemulsion mobile phase. The separations were performed on a C18 (4.6 × 250 mm, 5 μm) column at 25 °C using a flow rate of 1.0 mL/min, and an ultraviolet detection wavelength of 210 nm. The microemulsion mobile phase comprised 2.8% (w/v) sodium dodecyl sulfate (SDS), 7.0% (v/v) n-butanol, 0.8% (v/v) n-octane and 0.1% (v/v) aqueous orthophosphate buffer (H3PO4). The linearity analysis of tenuifolin showed a correlation coefficient of 0.9923 in the concentration range of 48.00–960.00 µg/mL. The accuracy of the method based on three concentration levels ranged from 96.23% to 99.28%; the limit of detection (LOD) was 2.34 µg/mL, and the limit of quantification (LOQ) was 6.76 µg/mL. The results of our study indicated that the optimized MELC method was sensitive and robust, and can be widely applied for the separation and determination of tenuifolin in Radix Polygalae.

show abstract

Optimization of liquid chromatographic method for the separation of nine hydrophilic and hydrophobic components in Salviae miltiorrhizae Radix et Rhizoma (Danshen) using microemulsion as eluent

Cited by 11 publications

References 28 publications

Surfactant‐coated graphitized multiwalled carbon nanotubes as the pseudostationary phase in electrokinetic chromatography for the analysis of phytochemical compounds in biological fluids

Surfactant‐coated graphitized multiwalled carbon nanotubes as the pseudostationary phase in electrokinetic chromatography for the analysis of phytochemical compounds in biological fluids

Determination of the lipophilicity of Salvia miltiorrhiza Radix et Rhizoma (danshen root) ingredients by microemulsion liquid chromatography: optimization using cluster analysis and a linear solvation energy relationship‐based method

A microemulsion high-performance liquid chromatography (MELC) method for the separation and determination of hydrolyzed tenuifolin in Radix Polygalae

Contact Info

Product

Resources

About