Optimization of lovastatin production by Aspergillus terreus ATCC 10020 using solid-state fermentation and its pharmacological applications

Al-Saman, Mahmoud A.; Helmy, Mai M.; Abdella, Asmaa; Wilkins, Mark R.; Gobba, Naglaa A.; Mahrous, Hoda

doi:10.1016/j.bcab.2021.101906

Cited by 11 publications

(10 citation statements)

References 37 publications

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“…Also, Peru is considered a diverse country that has a very broad microbial diversity richness, however, remains slightly studied and exploited [84,85]. Fungi, the eukaryotic microorganisms, produce a tremendous number of NPs with diverse chemical structures and biological activities [86], such as lovastatin, the first statin approved as a hypercholesterolemic medication by the FDA, most frequently produced by Aspergillus terreus [87], and cyclosporine A, a potent immunosuppressant that was initially used to prevent organ rejection, isolated from the fungal species Tolypocladium inflatum gams [88]. Besides that no current drug has been developed from propolis, it is considered a very rich and complex chemical composition, while about 300 different chemicals components isolated from it, and which composition fluctuates according to parameters such as plant source, seasons harvesting, geography, type of bee flora, climate changes, and honeybee species [89,90]; highlighting Artepillin C, extracted from Brazilian green propolis, that showed in vitro [91] and in vivo [92]anti-inflammatory potential.…”

Section: Discussionmentioning

confidence: 99%

PeruNPDB: The Peruvian Natural Products Database for in silico drug screening

Barazorda-Ccahuana

Ranilla

Candia-Puma

et al. 2023

Preprint

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Since the number of drugs based on natural products (NPs) represents a large source of novel pharmacological entities, NPs have acquired significance in drug discovery. Peru is considered a megadiverse country with many endemic species of plants, terrestrial, and marine animals, and microorganisms. NPs databases have a major impact on drug discovery development. For this reason, several countries such as Mexico, Brazil, India, and China have initiatives to assemble and maintain NPs databases that are representative of their diversity and ethnopharmacological usage. We describe the assembly, curation, and full chemoinformatic evaluation of the content and coverage in chemical space, as well as the physicochemical attributes and chemical diversity of the initial version of the Peruvian Natural Products Database (PeruNPDB), which contains 280 compounds. Access to PeruNPDB is available for free (https://perunpdb.com.pe/). The PeruNPDB collection is intended to be used in a variety of tasks, such as virtual screening campaigns against various disease targets or biological endpoints. This emphasizes the significance of biodiversity protection both directly and indirectly on human health.

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Section: Discussionmentioning

confidence: 99%

PeruNPDB: The Peruvian Natural Products Database for in silico drug screening

Barazorda-Ccahuana

Ranilla

Candia-Puma

et al. 2023

Preprint

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“…It is involved in the inhibition of cholesterol biosynthesis. Lovastatin also works as an in vivo competitive inhibitor of HMG-CoA reductase for humans and animals as well as in plants and is hence used to treat hypercholesterolemia 1,[3][4][5][6][7][8] . Lovastatin is available in the lactone form but converted to β-hydroxy acid invivo, the active state.…”

Section: Introductionmentioning

confidence: 99%

“…Lovastatin is available in the lactone form but converted to β-hydroxy acid invivo, the active state. Also, Lovastatin is the precursor of simvastatin 4 . Studies have shown that lovastatin can also increase good cholesterol or high-density lipoproteins in the blood, thereby preventing plaque formation 6 .…”

Section: Introductionmentioning

confidence: 99%

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Production of Lovastatin using Liquid Cheese Whey by Fusarium nectrioides (MH173849), an Endophytic Fungi Isolated from Euphorbia hirta

Manogaran¹,

Kannan²,

Narayanan³

et al. 2022

J. Pure Appl. Microbiol.

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Lovastatin is a naturally produced 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase enzyme inhibitor- used for treating hypercholesterolemia. It was the first statin drug which was approved by the United States Food and Drug Administration (USFDA). In the current study, endophytic fungus Fusarium nectrioides (MH173849) isolated from Euphorbia hirta L. was used for the production of lovastatin. Four different culture media indicated as M1, M2, M3 and M4 were used for the initial production of lovastatin. Liquid cheese whey was used as nitrogen source. Growth morphology of fungi was investigated using Scanning Electron Microscopy analysis. Also, parameters like temperature, pH, inoculum size, incubation time, and RPM were optimized for the obtaining highest lovastatin production. Among the four media, M4 was found to produce the maximum concentration of lovastatin. Parameters such as temperature of 28°C, pH 6, RPM – 180 rpm and inoculum size of 5 x107 spores/mL were optimal for the production of lovastatin by F. nectrioides (MH173849).

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“…Some scholars have also used Aspergillus spp. to ferment cardiovascular drugs (as statins; Barrios-González et al, 2020 ; Al-Saman et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Screening of Key Fungal Strains in the Fermentation Process of the Chinese Medicinal Preparation “Lianzhifan Solution” Based on Metabolic Profiling and High-Throughput Sequencing Technology

Xie

Yang

et al. 2021

Front. Microbiol.

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“Lianzhifan solution” (LZF) is produced by the natural fermentation of coptis root and gardenia fruit, and it is a classic prescription for external use in anorectal department. During the fermentation process, the structural evolution of microbial communities led to significant changes in the chemical profile. In this study, we first analyzed the dynamic changes of chemical components as well as the composition and succession of microbial community during the whole fermentation process of LZF, and confirmed the changes of characteristics of nine compounds during the whole fermentation process by metabolic profile. Further analysis found that there was no significant change of alkaloids in all stages of fermentation of LZF, but there were significant changes of iridoids in the middle and late stage of fermentation by deglycosylation. Genipin gentiobioside and geniposide were converted to genipin by biotransformation, showing that deglycosylation was the main event occurring in the fermentation. The community composition and abundance of species in 10 and 19days LZF fermentation broth were analyzed with high-throughput sequencing technology, and 16 dominant bacterial genera and 15 dominant fungal genera involved in the fermentation process were identified. Correlation analysis revealed that Penicillium expansum and Aspergillus niger involved in the fermentation were the dominant genera closely related to the dynamic changes of the deglycosylation of the main chemical components, and P. expansum YY-46 and A. niger YY-9 strains were obtained by the further fractionation. Then the monoculture fermentation process was evaluated, whereby we found that the deglycoside conversion rate of iridoid glycosides was greatly improved and the fermentation cycle was shortened by 3–4 times. This finding combined with equivalence evaluation of chemical component and pharmacodynamics to confirm that P. expansum YY-46 and A. niger YY-9 strains were key strains for fermentation concoction. This study established an efficient and practical screening strategy “Microfauna communities-Chemical component-Pharmacodynamic” axis for key strain, to improve the production process and formulating good manufacturing practice (GMP) work, and it is also applicable to the whole fermentation drugs industry.Graphical AbstractThe figure highly summarizes the research content of this study and shows the screening process of key strains in LZF fermentation.

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Optimization of lovastatin production by Aspergillus terreus ATCC 10020 using solid-state fermentation and its pharmacological applications

Cited by 11 publications

References 37 publications

PeruNPDB: The Peruvian Natural Products Database for in silico drug screening

PeruNPDB: The Peruvian Natural Products Database for in silico drug screening

Production of Lovastatin using Liquid Cheese Whey by Fusarium nectrioides (MH173849), an Endophytic Fungi Isolated from Euphorbia hirta

Screening of Key Fungal Strains in the Fermentation Process of the Chinese Medicinal Preparation “Lianzhifan Solution” Based on Metabolic Profiling and High-Throughput Sequencing Technology

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