Optimization of Lyophilized Hyperacute Serum (HAS) as a Regenerative Therapeutic in Osteoarthritis

Abstract

Hyperacute serum (HAS) is a blood derivative product that promotes the proliferation of various cell types and controls inflammation in vitro. The aim of this study is to investigate the regenerative potential of different formulations of HAS, including lyophilized and hyaluronic acid combined versions, to obtain a stable and standardized therapeutic in osteoarthritis (OA), which may be able to overcome the variability limitations of platelet-rich plasma (PRP). Primary human osteoarthritic chondrocytes were used for testing cellular viability and gene expression of OA-related genes. Moreover, a co-culture of human explants of cartilage, bone and synovium under inflammatory conditions was used for investigating the inflammatory control capacities of the different therapeutics. In this study, one formulation of lyophilized HAS achieved the high cell viability rates of liquid HAS and PRP. Gene expression analysis showed that HAS induced higher Col1a1 expression than PRP. Cytokine quantification from supernatant fluids revealed that HAS treatment of inflamed co-cultures significantly reduced levels of IL-5, IL-15, IL-2, TNFα, IL-7 and IL-12. To conclude, lyophilized HAS is a stable and standardized therapeutic with high potential in joint regeneration.

Professional Athletes Maintain High TNF-Alpha or IFN-Gamma Related Inflammatory Status after Recovering from COVID-19 Infection without Developing a Neutralizing Antibody Response

“…Analysis of large data matrices generated by 27 analytes in a high number of patients inevitably leads to omissions and combinations, making paper-to-paper comparison difficult—that is why we share the raw measurement data in Supplementary Table S1 to aid further research. Comparing the present data with others already available in the literature, it is apparent that the high correlation coefficients we present are way stronger than what we observed in other diseases or what is generally published with cytokine panels [ 28 ]. Specifically, this allows the recognition of meaningful cytokine-cytokine interactions that are otherwise masked in the wider variations of the non-athlete, general plasma donor population.…”

Hyperacute Serum and Knee Osteoarthritis

“…al. [16] In vitro Supplementation with lyophilized HS led to a significantly higher rate of cell proliferation, similar to that of freshly prepared HS. In addition, it led to a significant reduction in the expression levels of proinflammatory cytokines.…”

“…The in vitro studies demonstrated that treatment with HS resulted in increased viability of osteoarthritic chondrocytes and MSCs, reduced expression of pro-inflammatory cytokines, increased expression of antiinflammatory cytokines, increased differentiation of MSCs towards the chondrogenic lineage, and increased expression of chondrogenesis-related genes [11][12][13][14][15][16][17]. In addition, the cell-free and anti-coagulant-free nature of HS led to no variability in the differentiation potential or gene expression of MSCs, unlike PRP.…”

“…Calvo et al investigated the regenerative potential of lyophilized HS and HS in combination with hyaluronic acid (HA) [ 16 ]. OA chondrocytes were isolated from cartilage obtained from patients undergoing knee replacement surgery and tested for viability of cells and expression of genes related to OA.…”

A Strategic and Worldwide Cooperative Challenge Required for the Next Generation of Platelet Concentrates

“…This study meets the purpose of Project #3. Olmos Calvo et al demonstrated hyperacute serum, a cell and fibrin-free solution that is obtained after pressing out fibrin clots, retains its regenerative potential after the freeze-drying process [ 7 ]. This may be related to Project #3.…”