A Strategic and Worldwide Cooperative Challenge Required for the Next Generation of Platelet Concentrates

Kawase, Tomoyuki

doi:10.3390/ijms23073437

Cited by 4 publications

(7 citation statements)

References 9 publications

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“…If this is true, unlike the widely accepted understanding of PRP therapy, growth factor levels are not the predominant determinant of clinical outcomes. As previously claimed [65], the outcome of PRP therapy can be determined by the balance between recipient conditions and PRP quality based on the adjuvant principle (Fig. 3) [65].…”

Section: Clinical Relevancementioning

confidence: 96%

“…It is important for clinicians to acquire the ability to predict which patients will respond positively to PRP. Thus, we again propose that further attention should be paid to recipients' conditions to obtain an in-depth understanding of PRP therapy [65].…”

Section: Clinical Relevancementioning

confidence: 97%

“…As previously claimed [65], the outcome of PRP therapy can be determined by the balance between recipient conditions and PRP quality based on the adjuvant principle (Fig. 3) [65]. It is assumed that PRP therapy works successfully only when the recipient's condition, especially the regeneration activity, is maintained at moderate to high levels.…”

Section: Clinical Relevancementioning

confidence: 99%

“…However, it is difficult to appreciate that the clinicians' understanding has reached a standard level, as it notably varies among individuals [64]. Using a series of original and review articles [65][66][67][68][69][70], this study proposed important factors to focus on for better PRP therapy. However, to the best of our knowledge, no breakthrough advances have been achieved in this decade.…”

Section: Prospective View and Further Studiesmentioning

confidence: 99%

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The levels of TGFβ1, VEGF, PDGF-BB, and PF4 in platelet-rich plasma of professional soccer players: a cross-sectional pilot study

et al. 2022

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Background Regenerative therapy using platelet-rich plasma (PRP), a rich source of growth factors, has become popular in orthopedic sports medicine. Elite athletes prefer PRP therapy for their injured muscles and tendons primarily to avoid the possible risks of surgical treatment. However, the clinical effectiveness of PRP therapy in elite athletes compared to that in non-athletes remains unknown. Therefore, to investigate the effectiveness of PRP therapy in professional athletes (pro-athletes), we focused on the quality of PRP preparations and compared the levels of bioactive molecules between pro-athletes and non-athletes. Methods PRP was prepared from healthy, non-smoking male professional soccer players (pro-athletes) (n = 22) and non-athletes (VEGF: n = 34, others: n = 38). The levels of TGFβ1, PDGF-BB, VEGF, and PF4 were determined using ELISA kits. Polyphosphate was probed with 4’,6-diamidino-2-phenylindole and monitored using a fluorometer. The body composition of the donors was determined using a bathroom weighing scale. Results The levels of TGFβ1 and VEGF were significantly lower in pro-athletes than in non-athletes, whereas PF4 levels were significantly higher in pro-athletes. No significant difference was found in PDGF-BB levels between these groups. Biomolecule levels were not correlated with polyphosphate levels. Conclusion TGFβ1, VEGF, and PDGF-BB levels in pro-athletes were not higher than those in non-athletes. These findings suggest that growth factor levels in PRP may not be a predominant determinant of the clinical effectiveness of PRP therapy in pro-athletes. Increased PF4 levels in pro-athletes suggest an immunological function of PRP that may positively influence tissue regeneration.

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Section: Clinical Relevancementioning

confidence: 96%

Section: Clinical Relevancementioning

confidence: 97%

Section: Clinical Relevancementioning

confidence: 99%

Section: Prospective View and Further Studiesmentioning

confidence: 99%

See 2 more Smart Citations

The levels of TGFβ1, VEGF, PDGF-BB, and PF4 in platelet-rich plasma of professional soccer players: a cross-sectional pilot study

et al. 2022

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“…To better understand the unbalanced bioenergetics of dysfunctional mitochondria, it is important to elucidate the regulatory mechanism of polyP turnover and its pathophysiological involvement in mammalian bioenergetics. In addition, considering the stimulatory effects of polyP on blood coagulation, this approach is expected to improve platelet-rich plasma therapy, which is frequently used in regenerative medicine [ 22 , 23 ].…”

Section: Discussionmentioning

confidence: 99%

Modulation of ATP Production Influences Inorganic Polyphosphate Levels in Non-Athletes’ Platelets at the Resting State

Ushiki

Mochizuki

Suzuki

et al. 2022

IJMS

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Platelets produce inorganic polyphosphate (polyP) upon activation to stimulate blood coagulation. Some researchers have linked polyP metabolism to ATP production, although the metabolic linkage is yet to be elucidated. We found evidence for this possibility in our previous study on professional athletes (versus non-athletes), and proposed that the regulatory mechanism might be different for these two groups. To explore this aspect further, we investigated the effects of modulated ATP production on polyP levels. Blood samples were obtained from Japanese healthy, non-athletes in the presence of acid-citrate-dextrose. The platelets in the plasma were treated with oligomycin, rotenone, and GlutaMAX to modulate ATP production. PolyP level was quantified fluorometrically and visualized using 4′,6-diamidino-2-phenylindole. Correlations between polyP and ATP or NADH were then calculated. Contrary to the hypothesis, inhibitors of ATP production increased polyP levels, whereas amino acid supplementation produced the opposite effect. In general, however, polyP levels were positively correlated with ATP levels and negatively correlated with NADH levels. Since platelets are metabolically active, they exhibit high levels of ATP turnover rate. Therefore, these findings suggest that ATP may be involved in polyP production in the resting platelets of non-athletes.

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The value of platelet-rich plasma-derived extracellular vesicles in modern medicine

Hou,

Wen,

Zhou

et al. 2023

Annals of Medicine

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Platelet-rich plasma (PRP) has been widely used in clinical practice. The mechanism by which PRP promotes tissue repair lies in the release of multiple growth factors upon platelet activation, which accelerates the proliferation and differentiation of repair cells and the synthesis of extracellular matrix. In recent years, as extracellular vesicles (EVs) research has increased and intensified, it has been found that EVs also play an important role in tissue repair. This article provides a comprehensive review of the role of PRP and PRP-derived extracellular vesicles (PRP-EVs) in tissue repair. It discusses the biological characteristics, extraction, identification, activation, and preservation of PRP-EVs. It also reviews their applications in orthopedics and wound repair. The article highlights the importance of PRP-EVs in modern medicine and suggests that they could be a promising natural nanocarrier.

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A Strategic and Worldwide Cooperative Challenge Required for the Next Generation of Platelet Concentrates

Abstract: Blood-derived biomaterials, which are represented by platelet-rich plasma (PRP) or more recently by platelet-rich fibrin (PRF), have been used in regenerative therapy for almost 30 years [...]

Cited by 4 publications

References 9 publications

The levels of TGFβ1, VEGF, PDGF-BB, and PF4 in platelet-rich plasma of professional soccer players: a cross-sectional pilot study

The levels of TGFβ1, VEGF, PDGF-BB, and PF4 in platelet-rich plasma of professional soccer players: a cross-sectional pilot study

Modulation of ATP Production Influences Inorganic Polyphosphate Levels in Non-Athletes’ Platelets at the Resting State

The value of platelet-rich plasma-derived extracellular vesicles in modern medicine

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