Optimization of media composition and culture conditions for acarbose production by Actinoplanes utahensis ZJB-08196

Abstract

Acarbose is a clinically useful drug for the treatment of type II, insulin-independent diabetes as a hypoglycemic agent. An acarbose-overproducing strain ZJB-08196, indentified as Actinoplanes utahensis, was able to produce 4,210 mg l -1 of acarbose at 591 mOsm kg -1 with the optimized conditions at bench scale. Shake flask fermentation showed that maltose, glycerol and monosodium glutamate were supportive for acarbose production; soybean meal had higher bioavailability than corn steep liquor. Moreover, acarbose formation was not parallel with mycelial growth and the pattern of acarbose production by A. utahensis ZJB-08196 was the type of mixed-growth associated.

Influence of Ammonium Salt and Fermentation pH on Acarbose Yield from <i>Streptomyces</i> M37

“…That is, glutamate, glutamine and glucosamine stimulated the formation of antibiotics containing aminoglycoside group [25]. Medium pH affects enzyme synthesis, enzymatic processes, and transport across cell membranes [7]. The concentration of ammonia was primarily determined by the ammonium concentration, but under increasing pH the fraction of nitrogen in the form of ammonia increased.…”

“…Many aspects of acarbose fermentation have been well studied and reported, ranging from producer strains, media formula, fermentation conditions, and acarbose isolation and purification [5][6][7][8][9]. The importance of nitrogen sources in industrial microbiology is very significant since it affects the synthesis of enzymes involved in both primary and secondary metabolism [10][11][12].…”

“…It is known that glutamate is the primary source of Nglycosidic bond in the biosynthesis of acarbose [13]. Wang et al [7] found that raising monosodium glutamate (MG) concentration up to 5 g/L promoted acarbose production with A. utahensis ZJB-08196. However, the effects of inorganic nitrogen source and pH on acarbose fermentation have not been reported thus far.…”

Enhanced Production of Acarbose and Concurrently Reduced Formation of Impurity C by Addition of Validamine in Fermentation ofActinoplanes utahensisZJB-08196

“…A56 and developed an optimized industrial fermentation processes for acarbose production, as a result about 5000 mg/L of acarbose was obtained. Our group has extensively studied the production of acarbose [14–18]. A high acarbose-producing mutant stain A. utahensis ZJB-08196 was isolated by mutagenesis and screening method [14].…”

“…Our group has extensively studied the production of acarbose [14–18]. A high acarbose-producing mutant stain A. utahensis ZJB-08196 was isolated by mutagenesis and screening method [14]. Fed-batch fermentation with A. utahensis ZJB-08196 at elevated osmolality via intermittently feeding of necessary components regarding acarbose formation afforded a peak acarbose titer of 4878 mg/L [15].…”