Optimization of medium components for high-molecular-weight hyaluronic acid production by Streptococcus sp. ID9102 via a statistical approach

Abstract

Hyaluronic acid (HA), linear high-molecular-weight glycosaminoglycan produced from Streptococcus sp., has raised interest in the medical and cosmetics industries because of the various biological functions of HA. In this paper, we report on the optimization of medium components for HA production in Streptococcus sp. ID9102 (KCTC 11935BP) by two-step optimization (one-factor-at-a-time and taguchi orthogonal array design). In the first step, medium components, such as carbon, nitrogen, phosphate, and mineral sources, were selected for HA production in Streptococcus sp. ID9102 (KCTC 11935BP) using the one-factor-at-a-time method. In the second step, the concentration of the selected medium components was optimized using taguchi orthogonal array design. The design for medium optimization was developed and analyzed using MINITAB 14 software. In addition, the effect of amino acid and organic acid, such as glutamine, glutamate, and oxalic acid, was studied for HA production in Streptococcus sp. ID9102 (KCTC 11935BP). Through these processes, the optimum medium comprising 4% glucose, 0.75% yeast extract, 1.0% casein peptone, 0.25% K(2)HPO(4), 0.05% MgCl(2), 0.5% NaCl, 0.04% glutamine, 0.06% glutamate, and 0.02% oxalic acid was determined. We were able to produce HA with a molecular weight of 5.9 x 10(6) at a productivity of 6.94 g/l on pilot scale fermentation.

High cell density cultivation of Escherichia coli K4 in a microfiltration bioreactor: a step towards improvement of chondroitin precursor production

“…All the results of fermentation experiments confirmed the consistency of the microfiltration bioreactor technology as a valid tool in the biotechnological production of the chondroitin precursor, the E. coli K4 capsular polysaccharide. In the framework of this research we addressed the issue of the low yields reported for the microbial production of K4 CPS [22], permitting the achievement of titres similar to the ones obtained in the biotechnological production of the most noted glycosaminoglycan, that is hyaluronic acid [23]. …”

Hyaluronic acid production by Streptococcus zooepidemicus in marine by-products media from mussel processing wastewaters and tuna peptone viscera

“…The strategies and methods for improving HA fermentation have been focused on optimising culture conditions and the associated metabolic fluxes [18,35]. However, the possibility of using residual sources of carbon and organic nitrogen from food by-products in order to reduce the price of HA production is still unexplored.…”

“…Although several strategies have been reported for increasing microbial HA, including pH-gradient stress [8], continuous culture [9], lysozyme or hyaluronidase addition [10,11], agitation and aeration conditions [12-14], medium optimisation [15], the type of bioreactor [16], effect of aminoacids and mineral salts [17,18] and fed-batch operation [19], there is almost no studies of new sources of sugars and proteins from organic waste materials in order to reduce both production costs and pollution problems. More than 80% of these costs are due to these nutrients (sugars and proteins) and commercial formulations are not an economical resource for industrial production of HA.…”

OPTIMIZACIÓN DE LA PRODUCCIÓN DE LA ENZIMA L-ARABINOSA ISOMERASA POREnterococcus faecium DBFIQ E36 MEDIANTE TÉCNICAS ESTADÍSTICAS MULTIVARIADAS

“…Los extractos estériles fueron almacenados y posteriormente, se les determinó por triplicado, la actividad enzimática y la concentración de proteínas. Asimismo, cada experiencia fue realizada por duplicado, en forma independiente y al azar (Im et al, 2009). Finalmente, se utilizó como cultivo control del crecimiento y de la producción de la enzima, el crecimiento de la cepa seleccionada en medio MRS comercial.…”