Optimization of medium for indole-3-acetic acid production using Pantoea agglomerans strain PVM

Apine, Onkar A.; Jadhav, Jyoti P.

doi:10.1111/j.1365-2672.2011.04976.x

Cited by 108 publications

(59 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…2). Similar results were obtained with bacterial strains, namely Pantoea agglomerans PVM (Apine and Jadhav 2011), Streptomyces viridis CMU-H009 (Khamna et al 2010), Bacillus megaterium and Lactobacillus casei (Mohite 2013). However, Bacillus licheniformis produced higher amounts of IAA at 37 °C (Prashanth and Mathivanan 2010).…”

Section: Resultssupporting

confidence: 76%

Continuous production of indole-3-acetic acid by immobilized cells of Arthrobacter agilis

et al. 2017

View full text Add to dashboard Cite

Indole acetic acid (IAA) is a plant growth-promoting hormone used in agriculture; therefore, its continuous production is of paramount importance. IAA-producing eight bacteria were isolated from the rhizosphere of Verbascum vulcanicum. Among them, Arthrobacter agilis A17 gave maximum IAA production (75 mg/L) and this strain was used to immobilization studies. The A. agilis A17 cells were immobilized in calcium alginate for the production of IAA. Optimization of process parameters for IAA production was carried out to enhance IAA production using immobilized cells. The maximal production of IAA was 520 mg/L under the following optimal conditions: 1% mannitol, 30 °C, pH 8.0, and 24 h incubation. It was determined that the immobilized cells could be reused (13 times) for the production of IAA.

show abstract

Section: Resultssupporting

confidence: 76%

Continuous production of indole-3-acetic acid by immobilized cells of Arthrobacter agilis

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Thokal et al [26] have also assessed the effect of pH values 4, 5, 6, 7 and 8 and they found pH 7 as optimum for their cultures. Pantoea agglomerans produced maximum IAA production at pH 7 [27].…”

Section: Discussionmentioning

confidence: 95%

Plant growth promotion using microbial IAA producers in conjunction with azolla: a novel approach

Raut

Shaikh

Naphade

et al. 2017

Chem. Biol. Technol. Agric.

View full text Add to dashboard Cite

Studies were conducted to find efficient Indole acetic acid producing strains and assessing the effectiveness of combined dose of azolla (dried) and IAA producers on plant growth. Out of ten isolates, only two isolates, viz, F1 and G2 were found to be potent IAA producers and were used for further experiments. It was found that the isolate F1 and G2 produced maximum amount of IAA at 0.9% tryptophan and incubation for 72 h at room temperature. The optimum pH for IAA production for isolates F1 and G2 was found to be at pH 9. The isolates were also assessed for the presence of other plant growth promoting activity, such as hydrogen cyanide production, oxalate solubilization, phosphate solubilization and zinc solubilization. Effect of IAA producers on seed germination was done by pot experiments carried out into sets. Isolates F1 and G2 were found efficient for plant growth promotion in desired aspects. Effect of combined dose of azolla and IAA producers on seed germination was assessed by pot experiments. For this, dried powder of azolla was added during sowing of culture treated seeds. No further culture supply was given to the seeds. Azolla has high protein content. Slow protein releasing property of azolla can be useful to provide constant supply of the precursor (tryptophan) for IAA synthesis by isolate. In addition to the plant exudates, azolla may also give constant supply of tryptophan. From all the assessed samples, the isolates FI and G2 showed efficient growth of plants in all aspects. G2 was found to be more potent than F1. So as to assess the compatibility of isolate in natural conditions, field studies were done. Four plots designed having individual size 9 × 5 m, were used. It was found that plots 3 (only culture) and 4 (culture with azolla) showed better plant growth as compared to others. On the basis of IAA producing ability of G2, pot experiments and field studies, it was concluded that isolate G2 can be used alone or in conjunction with azolla as a biofertilizer to get better plant growth.

show abstract

“…The production of IAA was directly proportional to the incubation time and after 2 days of incubation IAA production enhanced by twofold, while in authors' own research presented in this paper, the amount of produced IAA doubled only after 7 days of incubation. Apine and Jadhav [39] obtained much higher concentrations of IAA. However, an inversed relationship was observed in this case, as with the increase in L-tryptophan concentration in the medium, the amount of determined IAA decreased.…”

Section: Resultsmentioning

confidence: 99%

“…2). In order to determine the abilities to produce IAA by tested strains P. agglomerans BC17 and BC45, the optimal conditions for bacterial growth was provided, that is 30 °C and pH 7, which was confirmed by Apine and Jadhav [39] in their research on the optimization of biosynthesis of IAA by P. agglomerans strain PVM. Under the same conditions, the authors obtained maximum amount of IAA 1530 µg/cm 3 at 30 °C and maximum amount of IAA 1441 µg/cm 3 at pH 7.…”

Section: Resultsmentioning

confidence: 99%

“…Under these conditions the isolate was able to produce 59 µg/cm 3 of IAA. Apine and Jadhav [39] also analysed the effect of incubation time to optimize culturing conditions. The production of IAA was directly proportional to the incubation time and after 2 days of incubation IAA production enhanced by twofold, while in authors' own research presented in this paper, the amount of produced IAA doubled only after 7 days of incubation.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Importance of Endophytic Strains Pantoea agglomerans in the Biological Control of Rhizoctonia solani

Nabrdalik

Moliszewska

Wierzba

2018

Ecological Chemistry and Engineering S

View full text Add to dashboard Cite

Antagonistic activity of microorganisms against phytopathogens is mainly the results of plants' health improvement due to the inhibition of pathogens growth and the induction of plants resistance against diseases. The aim of the research was to determine antagonistic properties of Pantoea agglomerans against Rhizoctonia solani. The properties of two strains P. agglomerans BC17 and BC45 were assessed according to the following criteria: mycelial growth of R. solani in the presence of bacterial metabolites, an impact of P. agglomerans on the growth of sugar beet in the pots containing soil with and addition of R. solani and without it, the ability to produce indole-3-acetic acid (IAA). It has been recorded that antagonistic properties of tested strains are different. In the presence of metabolites of BC17 strains, the mycelial growth of R. solani was inhibited by 78 % and for the strain BC45 the value amounted 46 %. In the pot bioassay the number of infested plants growing in the soil inoculated with P. agglomerans and the pathogen was lower when compared with the pots containing R solani. A higher reduction of infested plants, amounting 23 %, was obtained for the strain BC17. Both strains had the ability to produce IAA -a plant hormone of the auxin class, in the presence of tryptophan and its absence in the medium. The highest concentration of IAA was recorded after 7 days of culturing in the supernatant obtained from the media containing 2000 µg/cm 3 of tryptophan. For the strain BC17 the concentration of IAA marked in the postculturing liquid amounted 71.57 µg/cm 3 , and for the BC45 strain it amounted over 80 µg/cm 3 . Obtained results prove that P. agglomerans may be used in the biological protection against phytopatogenic strains of R. solani.

show abstract

Optimization of medium for indole-3-acetic acid production using Pantoea agglomerans strain PVM

Cited by 108 publications

References 23 publications

Continuous production of indole-3-acetic acid by immobilized cells of Arthrobacter agilis

Continuous production of indole-3-acetic acid by immobilized cells of Arthrobacter agilis

Plant growth promotion using microbial IAA producers in conjunction with azolla: a novel approach

Importance of Endophytic Strains Pantoea agglomerans in the Biological Control of Rhizoctonia solani

Contact Info

Product

Resources

About