Optimization of micellar LC conditions for separation of opium alkaloids and their determination in pharmaceutical preparations

Kulikov, A. Yu.; Boichenko, Alexander P.; Verushkin, Aleksey G.

doi:10.1039/c1ay05389b

Cited by 12 publications

(4 citation statements)

References 21 publications

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“…The optimization procedure [23,24] that was used for choosing the optimal mobile phase composition showed that for all micellar mobile phases were obtained good chromatographic characteristics: sesquiterpenic acid resolution was more than 1.5, and peak symmetry was 1.5-2. Lowest retention time (12 min) was obtained when 8% (w/v) of Brij 35 and 10% (v/v) of 1-BuOH as micellar mobile phase components were used.…”

Section: Resultsmentioning

confidence: 99%

Micellar LC Separation of Sesquiterpenic Acids and Their Determination in Valeriana officinalis L. Root and Extracts

Kulikov

2012

Chromatography Research International

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A simple micellar liquid chromatography (MLC) method was developed and validated according to ICH Guidelines for the determination of sesquiterpenic acids (valerenic, hydroxyvalerenic, and acetoxyvalerenic acids) in root and rhizome extract fromValeriana officinalisL. and valerian dry hydroalcoholic extract. Samples were analyzed on Nucleosil C18 column (, 5 μm) using an isocratic mobile phase which consisted of Brij 35 (5% (w/v) aqueous solution; pH by phosphoric acid) and 1-butanol (6% (v/v)); UV detection was at 220 nm. Micellar mobile phase using allows to fully separate valerenic acids within 25 minutes. Linearity for hydroxyvalerenic, acetoxyvalerenic, and valerenic acids was 1.9–27.9, 4.2–63.0, and 6.1–91·3 μg.mL−1, and limit of detection was 0.14, 0.037, and 0.09 μg·mL−1, respectively. Intraday and interday precisions were not less than 2% for all investigated compounds. The proposed method was found to be reproducible and convenient for quantitative analysis of sesquiterpenic acids in valerian root and related preparations.

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Section: Resultsmentioning

confidence: 99%

Micellar LC Separation of Sesquiterpenic Acids and Their Determination in Valeriana officinalis L. Root and Extracts

Kulikov

2012

Chromatography Research International

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“… [ 33 ] (3.0 mm × 150 mm; 3.5 mm), water (H 2 O/triethylamine) - mobile phase-A methanol (CH 3 OH/triethylamine) morphine, codeine, oripavine, noscapine n.a. RP-HPLC (MLC) column—C-18 (150 mm × 4.6 mm, 5 µm), spectrophotometric detector LOD-3.3 s/m LOQ-10 s/m [ 34 ] morphine GC-MS silica capillary column, carrier gas—helium, column (5 µm, 30 m × 0.25 mm) detector voltage −1700 n.a. [ 35 ] …”

Section: Extraction and Analytical Techniques Of Different Alkaloids ...mentioning

confidence: 99%

Opium alkaloids, biosynthesis, pharmacology and association with cancer occurrence

et al. 2023

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Papaver somniferum L. (Family: Papaveraceae) is a species well known for its diverse alkaloids (100 different benzylisoquinoline alkaloids (BIAs)). L-tyrosine serves as a precursor of several specific metabolites like BIAs. It has been used as an antitussive and potent analgesic to alleviate mild to extreme pain since ancient times. The extraction of pharmaceutically important alkaloids like morphine and codeine from poppy plant reflects the need for the most suitable and standard methods. Several analytical and extraction techniques have been reported in open literature for morphine, codeine and other important alkaloids which play a vital function in drug development and drug discovery. Many studies suggest that opioids are also responsible for adverse effects or secondary complications like dependence and withdrawal. In recent years, opium consumption and addiction are the most important risk factors. Many evidence-based reviews suggest that opium consumption is directly linked or acts as a risk factor for different cancers. In this review, we highlight significant efforts related to research which have been done over the past 5 decades and the complete information on Papaver somniferum including its phytochemistry, pharmacological actions, biosynthetic pathways and analytical techniques of opium alkaloid extraction and the link between opium consumption and cancer-related updates.

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“…Table 2 lists the different, recent applications of MLC for analysis of drugs in both pharmaceutical and biological matrices [ 92 , 93 , 94 , 95 , 96 , 97 , 98 , 99 , 100 , 101 , 102 , 103 , 104 , 105 , 106 , 107 , 108 , 109 , 110 , 111 , 112 , 113 , 114 , 115 , 116 , 117 , 118 ]. Although most of these methods used SDS as a surfactant, some of them also used Brij-35 [ 92 , 93 ], cetyltrimethylammonium bromide (CTAB) [ 94 ], or Tween-20 [ 95 ].…”

Section: Micellar Liquid Chromatographymentioning

confidence: 99%

Greening Reversed-Phase Liquid Chromatography Methods Using Alternative Solvents for Pharmaceutical Analysis

et al. 2018

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The greening of analytical methods has gained increasing interest in the field of pharmaceutical analysis to reduce environmental impacts and improve the health safety of analysts. Reversed-phase high-performance liquid chromatography (RP-HPLC) is the most widely used analytical technique involved in pharmaceutical drug development and manufacturing, such as the quality control of bulk drugs and pharmaceutical formulations, as well as the analysis of drugs in biological samples. However, RP-HPLC methods commonly use large amounts of organic solvents and generate high quantities of waste to be disposed, leading to some issues in terms of ecological impact and operator safety. In this context, greening HPLC methods is becoming highly desirable. One strategy to reduce the impact of hazardous solvents is to replace classically used organic solvents (i.e., acetonitrile and methanol) with greener ones. So far, ethanol has been the most often used alternative organic solvent. Others strategies have followed, such as the use of totally aqueous mobile phases, micellar liquid chromatography, and ionic liquids. These approaches have been well developed, as they do not require equipment investments and are rather economical. This review describes and critically discusses the recent advances in greening RP-HPLC methods dedicated to pharmaceutical analysis based on the use of alternative solvents.

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Optimization of micellar LC conditions for separation of opium alkaloids and their determination in pharmaceutical preparations

Cited by 12 publications

References 21 publications

Micellar LC Separation of Sesquiterpenic Acids and Their Determination in Valeriana officinalis L. Root and Extracts

Micellar LC Separation of Sesquiterpenic Acids and Their Determination in Valeriana officinalis L. Root and Extracts

Opium alkaloids, biosynthesis, pharmacology and association with cancer occurrence

Greening Reversed-Phase Liquid Chromatography Methods Using Alternative Solvents for Pharmaceutical Analysis

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