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Micellar liquid chromatography (MLC) has been developed for the analysis of opium alkaloids. The composition of micellar mobile phase for the separation of morphine, codeine, papaverine and noscapine have been optimized by a chemometric tool taking into account the efficiency and asymmetry of chromatographic peaks, resolution between each pair of peaks and total time of separation. The optimized mobile phase composition (0.10 M sodium dodecylsulfate (SDS), 5% (v/v) 1butanol; pH 2.5) allows to fully separate alkaloids within 15 min. The proposed MLC method was validated, compared with the RP-HPLC method and successfully applied for the analysis of the pharmaceutical preparation ''Omnopon, solution for injection''.

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A micellar thin-layer chromatography method for the quantitative determination and validation of coumarin in Meliloti herba and its ethanolic extracts was developed and validated. For achieving good determination, the mobile phase of 5 x 10-4 mol/L Tween-80 in a mixture propanol-2 – water (5:95 v/v) was used. Densitometric determination was carried out at 275 nm. The calibration curve was linear in the range of 0.1-2.5 μg per band. The proposed method is simple, rapid, precise and accurate; replacing hazardous solvents by greener ones correspond to the modern requirements in “Green chemistry” concepts. The obtained data can be used for the routine analysis of coumarin in medical plant and extracts.