Optimization of Micropropagation Protocol for Goji Berry (Lycium barbarum L.)

Fira, Alexandru; Joshee, Nirmal; Cristea, Vasile; Simu, Manuela; Hârța, Monica; Pamfil, Doru; Clapa, Doina

doi:10.15835/buasvmcn-hort:12177

Cited by 10 publications

(10 citation statements)

References 12 publications

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“…Results from this study agree with previous reports that BAP was the effective cytokinin for in vitro shoot multiplication of wolfberry (Fira et al, 2016), and the changes in the type and concentration of cytokinin applied can drastically affect shoot proliferation. The presence of callus formation and hyperhydricity at higher concentrations of BAP has been observed; these findings are confirmed in other species, where a dosedependent manner of cytokinins involved in hyperhydricity has been observed (Liu et al, 2017).…”

Section: Resultssupporting

confidence: 92%

“…The optimization of in vitro culture conditions through the study of basal salts and growth regulators has an important impact on the rooting and acclimatization processes. Previous reports described the use of MS medium (Fira et al, 2016), but in our experiments, the use of these basal salts, although achieving good results in terms of plant growth, during the second and third subculture, the shoots showed detrimental effects, characterized by a drastic growth reduction and hyperhydricity (data not shown). Our results suggest that the DKW medium is the most appropriate medium among the media tested; its effectiveness in shoot induction and development could be related to the mineral composition and their concentrations and, as for many woody species, Lycium barbarum prefers media with low ion concentrations.…”

Section: Resultscontrasting

confidence: 46%

“…Wolfberry plants often are reproduced through seeds and show all the problems related to sexual propagation, i.e., low germination, absence of clonal fidelity, and nonuniform agronomical performance. Despite the increasing interests in goji cultivation, there are few papers in the scientific literature regarding the micropropagation of wolfberry (Fira et al, 2016), and almost all described micropropagation protocols start from seedlings. Our previous experiments suggested that the major problems hampering the development of in vitro propagation protocols are the deleterious postexposure effect of wolfberry varieties on Murashige and Skoog (MS) medium and the nonuniformity of the explants used for rooting induction.…”

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confidence: 99%

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Micropropagation and Ex Vitro Rooting of Wolfberry

Silvestri¹,

Sabbatini²,

Marangelli³

et al. 2018

horts

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Additional index words. Lycium barbarum, axillary shoot proliferation, in vitro and ex vitro rooting, acclimatization, growth regulatorsAbstract. An accurate protocol for the in vitro propagation of a commercial wolfberry (Lycium barbarum L.) cv. Nixia 1 has been developed through axillary shoot proliferation. Driver and Kuniyuki Walnut (DKW) medium supplemented with 6-benzylaminopurine (BAP; 0.5 mg/L) and sucrose 3% w/v gave the best results compared with other basal media tested, with significantly improved production of multiple shoots direct from nodal segment explants, resulting in an average of 6.73 shoots/explant with an average of 7.45 nodes/shoot that would potentially form new explants. Rooting of shoot explants was carried out both in vitro and ex vitro with 0.5 and 1 mg/L of indole-3-butyric acid (IBA), with or without adding putrescine (160 mg/L). In all cases, rooting efficiency resulted very high, and putrescine was effective only when combined with a low concentration of auxin. Plantlets were hardened off in jiffy pots under greenhouse conditions, with a survival rate of more than 90%. Ex vitro rooting, performed by dipping in an aqueous solution of IBA 100 mg/L, is the preferred technique not only because rooting and acclimatization are very high but also reducing micropropagation to one phase is more economical.

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Section: Resultssupporting

confidence: 92%

Section: Resultscontrasting

confidence: 46%

mentioning

confidence: 99%

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Micropropagation and Ex Vitro Rooting of Wolfberry

Silvestri¹,

Sabbatini²,

Marangelli³

et al. 2018

horts

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“…In both cases, the regeneration rate was higher than that obtained by Fira et al (2016) for Lycium barbarum 'Ningxia N1', and by Tabăra (2017) for L. barbarum. Our results showed that WPM was the best medium for stimulating shoot length and the development of adventitious buds of goji.…”

Section: Resultsmentioning

confidence: 51%

“…Regeneration capacity depends on genotype, composition of growth media, plant growth regulators (PGRs), and other organic substances (Karakas, 2020). Several studies have tested various culture media for goji (Fira et al, 2016;Tabăra, 2017;Karakas, 2020;Kruczek et al, 2020b). Although in vitro culture methods and conditions are similar for different goji genotypes, their requirements of growth regulators in culture media are different.…”

Section: Academicpresmentioning

confidence: 99%

Micropropagation, rooting, and acclimatization of two cultivars of goji (Lycium chinense)

Kruczek

Krupa-Małkiewicz

Ochmian

2021

Not Bot Horti Agrobo

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In recent years, Lycium chinense (goji) has become increasing popular because of its public acceptance as a “superfood”. Hence, the present study aimed to develop a rapid production technology by using in vitro culture to produce plants with high health value, throughout year and in desired quantities. A micropropagation protocol for growing L. chinense ‘No 1’ and ‘New Big’ cultivars was developed. The explants were grown on MS medium supplemented with different concentrations of meta-Topolin (0.4-0.8 mg L-1), and WPM and RA without plant hormones. Among the tested combinations, the maximum regeneration rate (95-97%) with the mean shoot length of 3.53-4.12 cm and mean shoot number of 1.42–1.58 (‘No 1’ and ‘New Big’, respectively) was recorded for plants grown on MS with 0.6 mg L-1 mT and WPM. For in vitro rooting, healthy roots (4.71-4.91 cm) were obtained on MS with the addition of 20 ppm chitosan. A maximum of 70–80% plantlets (‘No 1’ and ‘New Big’, respectively) regenerated on the medium with chitosan were successfully acclimatized and established in the mixture of 90% peat and 10% perlite under field conditions.

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In vitro propagation and improving accumulation of coumarin in Lycium barbarum, a rare plant in the flora of Egypt

Nower

2022

Bull Natl Res Cent

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Background The conservation of natural populations of plants is very important for maintaining biological diversity. Lycium barbarum (goji berries) is classified as a very rare plant in the Egyptian flora. Goji berry is a fruit commonly known as ‘super-fruit,’ due to its beneficial constituents and medicinal properties. The aim of this study was to determine the influence of media formula added with various cytokinins on axillary bud induction of L. barbarum from shoot tip. An additional objective of this study was to investigate the effect of 2,4-D, TDZ, zeatin, IBA and NAA on growth and development of the plant through different stages. The present study also aims to improve coumarin accumulation as one of the most active constituents in the plant. Results Sterilized shoot tips of L. barbarum were cultured without growth regulators on different media formulas: Murashige and Skoog basal medium (MS medium), Gamborg’s medium (B5 medium) and Woody plant medium (WP medium). MS medium gave the best shoot induction. The maximum multiplication rate of shoots was recorded on MS medium supplemented with 225.24 µM 6-benzyl amino purine (BAP). The best callus induction rate from in vitro derived young leaves was obtained on MS medium with 552.60 µM thidiazuron (TDZ). At 176.20 µM TDZ, the maximum shoot proliferation ability was obtained through organogenesis from the callus. MS medium with 220.25 µM TDZ produced the highest number (15 buds) and forming rate (96%) of adventitious buds through direct organogenesis. On MS medium with 304.86 µM naphthalene acetic acid (NAA), 100% rooting was obtained. Almost 95% of the in vitro-generated plantlets were successfully acclimatized. Furthermore, suspension culture containing MS medium with 552.60 µM TDZ and cinnamic acid at a concentration of 763 μM increased coumarin synthesis (1.95-fold), compared to control. Conclusion The in vitro regeneration of plants via leaf-mediated shoot organogenesis protocol presented in this study is well suited for L. barbarum propagation and produced large quantities of identical planting materials for desert revegetation and pharmaceutical industries. A very effective indirect regeneration method via callus culture provides a potential for this ethnomedicinal plant to be improved by genetic transformation procedures, in addition to mass propagation of elite plants. The cell suspension culture medium with 763 µM cinnamic acid had the highest coumarin accumulation.

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Optimization of Micropropagation Protocol for Goji Berry (Lycium barbarum L.)

Cited by 10 publications

References 12 publications

Micropropagation and Ex Vitro Rooting of Wolfberry

Micropropagation and Ex Vitro Rooting of Wolfberry

Micropropagation, rooting, and acclimatization of two cultivars of goji (Lycium chinense)

In vitro propagation and improving accumulation of coumarin in Lycium barbarum, a rare plant in the flora of Egypt

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