Optimization Of Microwave-Assisted Extraction Of Flavonoids From Binahong (Anredera cordifolia) Leaves Using Respon Surface Methodology

Abstract

Binahong (Anredera scandens (L.) Moq.) leaves are one of the plants that empirically can be used for wound healing and contain flavonoids which have antibacterial and antioxidant activities. Determination of the optimum conditions of the Binahong leaf extraction process needs to be done to ensure the extraction quality of the Binahong leaves associated with the given activity. Microwave Assisted Extraction (MAE) is an extraction that utilizes microwave radiation to heat the solvent quickly and efficiently. This study aims to determine the most effective binahong leaf extraction conditions that produce optimal levels of flavonoids and antioxidant activity. The extraction process was carried out using the Microwave assisted extraction (MAE) method. Determination of optimum conditions is done based on Response Surface Method (RSM) with variable ethanol concentration (70%, 80% and 90%), extraction time (4, 12 and 20 minutes) and power (450, 600, and 800 watts) using Box- Behnken Design (BBD) with Design Expert 7.0 software. The extract quality parameters measured were total flavonoid levels and antioxidant activity. The results showed the optimum conditions with the BBD method were obtained at an ethanol concentration of 81.49%, extraction time of 13.84 minutes, and power of 626.19 watts with flavonoid levels of 3.8561% and antioxidant activity (IC50) of 95.51834 ppm with active categories.

Binahong leaf extract (anredera cordifolia) mucoadhesive patch as an alternative therapy for recurrent aphthous stomatitis

“…The advantage of using the MAE method is that it helps increase the amount of yield of the crude extract in a short time and the amount of solvent is relatively small compared to the conventional extraction method. 17 In addition, there is an ultrasonic-assisted extraction method by providing ultrasonic vibrations >20kHz on the surface of the simplicia. 19 Various Binahong leaf extraction methods with ethanol solvent with different concentrations can produce different yields (Table 4).…”

“…Cell hemolysis eventually occurs and causes the killing of bacteria in the ulcer and will not inhibit the ulcer healing process 11. Meanwhile, the potential for saponins as antioxidants is shown through their ability to reduce superoxide through the formation of hydroperoxide intermediates to prevent biomolecular damage caused by free radicals 17.…”

Effect of Solvent Type and Extraction Time on Binahong Leaf Extraction Process

“…Binahong leaves have several benefits including lowering cholesterol [3], as an anti-bacterial [4], and lowering uric acid in the blood [5]. The results of binahong leaf extraction that has been carried out previously show that binahong leaves contain flavonoids, alkaloids, saponins, and tannins [6]. This is supported by the statement of Waji and Andis [2] that antioxidants can be obtained from flavonoid compounds, phenolics, alkaloids, saponins, steroids, and triterpenoids.…”