Optimization of on-chip bacterial culture conditions using the Box-Behnken design response surface methodology for faster drug susceptibility screening

Li, Hua; Driesche, Sander van den; Bunge, Frank; Yang, Bin; Vellekoop, Michael J.

doi:10.1016/j.talanta.2018.10.048

Cited by 40 publications

(23 citation statements)

References 45 publications

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“…Therefore, the results of the whole experimental design matrix were analyzed by an RSM and a mathematical model was obtained Equation (1). This is a second-order polynomial equation, in which the response of the system is considered as a function of the factors and the interactions involved [31,34].…”

Section: Run Factorsmentioning

confidence: 99%

Optimization of Analytical Ultrasound-Assisted Methods for the Extraction of Total Phenolic Compounds and Anthocyanins from Sloes (Prunus spinosa L.)

et al. 2020

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Prunus spinosa L. is a shrub that produces berries—sloes—with a high content of phenolic compounds and anthocyanins. Numerous consumer products are made from sloes, such as “Pacharán” liquors or jams. For these reasons, it is crucial to design accurate analytical methods for the extraction of these bioactive compounds in order to promote their consumption and to improve our health. In this study, an ultrasound-assisted extraction method was developed to extract phenolic compounds and anthocyanins from sloes. Several process variables (methanol composition, pH, temperature, cycle, amplitude, and sample–solvent ratio) were optimized based on a Box–Behnken design. The most influential parameters for the extraction of total phenolic compounds were the cycle, the percentage of methanol, and pH of the extraction solvent, with 0.2 s, 67%, and pH 7 as their optimal values, respectively. For total anthocyanins, all parameters were influential, with 44% methanol at pH 2, 48 °C, 0.2 s cycles, 30% amplitude, and 1.5 g/20 mL ratio as the optimal values for their corresponding variables. Both methods showed high recoveries, short time use, and high precision (RSD < 5%). In addition, the sloe liqueur “Pacharán” and sloe jams were analyzed to demonstrate the applicability of these methods for the extraction of the real matrix.

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Section: Run Factorsmentioning

confidence: 99%

Optimization of Analytical Ultrasound-Assisted Methods for the Extraction of Total Phenolic Compounds and Anthocyanins from Sloes (Prunus spinosa L.)

et al. 2020

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“…Polymyxin B, an antibiotic primarily used to treat resistant bacterial infections, was selected as a model antibiotic in the view of development a microdevice to test bacterial resistance. The microbial resistance to antibiotics represents a huge well-known problem in medicine related to the excessive use of antibiotics as well as unhealthy life style [40]. It is interesting that a lot of NPs have antibacterial activity [41], however, resistance of bacteria to…”

Section: Introductionmentioning

confidence: 99%

Study of interactions between carboxylated core shell magnetic nanoparticles and polymyxin B by capillary electrophoresis with inductively coupled plasma mass spectrometry

Baron

Rozsypal

Michel

et al. 2020

Journal of Chromatography A

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In this work, interactions of carboxylated core shell magnetic nanoparticles with polymyxin B sulfate were studied by connecting capillary electrophoresis with inductively coupled plasma mass spectrometry. The interaction was probed by affinity mode of capillary electrophoresis with 25 mM phosphate buffer at physiological pH. 54 Fe, 56 Fe, 57 Fe, 34 S, and 12 C isotopes were used to monitor the migration of an electroosmotic flow marker and the interaction of the nanoparticles with polymyxin B. The analysis of interaction data showed two distinct interaction regions, one with low polymyxin B concentration, the second with high polymyxin B concentration. These regions differed in the strength of the interaction, 14.9 x 10 6 M-1 and 16.0 x 10 3 M-1 , and in the stoichiometry of 0.7 and 3.5, respectively. These differences can be explained by the decrease of electrostatic repulsion between nanoparticles caused by polymyxin B. This is also in agreement with the nanoparticles peak shapes: sharp for low polymyxin B concentrations and broad for high polymyxin B concentrations.

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“…The center point (0, 0, 0) was repeated five times, and the same predicted value was obtained each time, demonstrating a perfect fit between the factors and response. The precise values of the five replicates differed slightly, but within the margin of error . Using the Design Expert software, a multiple regression analysis of the experimental data was performed to obtain the coefficients and the corresponding equation to estimate the response.…”

Section: Resultsmentioning

confidence: 99%

“…The precise values of the five replicates differed slightly, but within the margin of error. 19 Using the Design Expert software, a multiple regression analysis of the experimental data was performed to obtain the coefficients and the corresponding equation to estimate the response. The obtained secondorder polynomial equation shows the relationship between the yield of L-PPT and the three variables:…”

Section: Optimum Reaction Time Of Racemizationmentioning

confidence: 99%

Efficient racemization of N‐phenylacetyl‐D‐glufosinate for L‐glufosinate production

Xue

et al. 2019

Chirality

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Most amino acids contain chiral centres and exist as both D‐enantiomer and L‐enantiomer. The optically pure enantiomer is often more valuable than the racemate. Enzymatic resolution provides an effective strategy to obtain optically pure amino acids but often results in large amounts of unwanted isomer. In this study, optically pure L‐glufosinate (L‐PPT) was obtained by coupling amidase‐mediated hydrolysis of N‐phenylacetyl‐D,L‐glufosinate with racemization of N‐phenylacetyl‐D‐glufosinate (NPDG), which exclusively exhibits effective herbicidal properties compared with its D‐enantiomer. To improve the yield of L‐PPT, the racemization reaction conditions were optimized, and through single‐factor experiments, the optimal reaction temperature, reaction time, and mole ratio of phenylacetic acid to NPDG were determined to be 150°C, 30 minutes, and 1.5, respectively. The response surface methodology was applied to further optimize the racemization conditions, and the final yield of L‐PPT reached 96.13% with optimum reaction temperature of 154°C, reaction time of 23 minutes, and phenylacetic acid/NPDG mole ratio of 1.7, respectively. Moreover, adding a small amount of acetic anhydride further raised the yield of L‐PPT to 97.02%.

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Optimization of on-chip bacterial culture conditions using the Box-Behnken design response surface methodology for faster drug susceptibility screening

Cited by 40 publications

References 45 publications

Optimization of Analytical Ultrasound-Assisted Methods for the Extraction of Total Phenolic Compounds and Anthocyanins from Sloes (Prunus spinosa L.)

Optimization of Analytical Ultrasound-Assisted Methods for the Extraction of Total Phenolic Compounds and Anthocyanins from Sloes (Prunus spinosa L.)

Study of interactions between carboxylated core shell magnetic nanoparticles and polymyxin B by capillary electrophoresis with inductively coupled plasma mass spectrometry

Efficient racemization of N‐phenylacetyl‐D‐glufosinate for L‐glufosinate production

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