Optimization of Phenolics Extracted from Idesia polycarpa Defatted Fruit Residue and Its Antioxidant and Depigmenting Activity In Vitro and In Vivo

Abstract

Extraction of phenolics from Idesia polycarpa defatted fruit residue was optimized by the maximization of the yield in total phenolics, using the response surface methodology. The optimized conditions were 50% ethanol, 5 h extraction time, 1 : 40 liquid to solid ratio, and 80°C extraction temperature. The experimental average total phenolics yield was 54.49 ± 4.26 mg/g. These antioxidant properties of phenolics were comprehensively analyzed for the first time. All the extracts not only demonstrated the significant free radical scavenging activities and metal chelating activity but also inhibited lipid, lipoprotein peroxidation and revealed reducing power activity. Ethyl acetate extraction (EAE) also inhibited mushroom tyrosinase activity and significantly increased the average skin-whitening index (L value) of the skin of C57BL/6 mice, indicating its potential use for skin hyperpigmentation in humans. The results of cell experiments showed EAE could strongly inhibit cellular tyrosinase activity, which had led to the decrease of melanogenesis in B16 mouse melanoma cells. Overall, EAE is an excellent natural antioxidant and depigmenting agent, which can be developed as a new food additive, medicine, and cosmetic.

Amelioration by Idesia polycarpa Maxim. var. vestita Diels. of Oleic Acid-Induced Nonalcoholic Fatty Liver in HepG2 Cells through Antioxidant and Modulation of Lipid Metabolism

“…The relative report has been confirmed that the oil of its fruits is nontoxic and has anticarcinogenic and antidiabetic effects and antihypertensive properties [ 12 , 13 ]. Moreover, flavonoid and phenolic glycosides of I. polycarpa exhibited various biological activities, such as antioxidant, skin whitening, inhibition of platelet aggregation, and antiadipogenesis [ 14 ]. Previous researches demonstrated that the ethyl acetate extracts of I. polycarpa exhibited strong antioxidant [ 14 ], anti-inflammatory, and whitening activities in vitro and in vivo [ 15 , 16 ].…”

Effects of Fermented Idesia Polycarpa Residues supplementation on laying performance of Laying Quails

“…There have been several studies on Idesia polycarpa fruit, including functional research on Idesia polycarpa fruit extraction and studies on the improvement of the extraction process. The remarkable antioxidant qualities, anti-skin-aging ability (3) and anti-adipogenic ability (4) of Idesia polycarpa extracts have been demonstrated, and an optimized extraction method has also been established (5). However, reports on the application of Idesia polycarpa fruit for the other purposes are still limited.…”