Optimization of phytase production by solid substrate fermentation

Bogar, Barbara; Szakács, George; Linden, James C.; Pandey, Ashok; Tengerdy, Robert P.

doi:10.1007/s10295-003-0027-3

Cited by 83 publications

(59 citation statements)

References 22 publications

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“…Products rich in phytase enzyme also contain some other hydrolytic enzymes that favor digestion in non-ruminants. This was reported by Bogar et al (2003) who found alpha amylase, xylanase and others. The supplementation of these enzymes together with phytase contributes to a decrease in the viscosity of food during digestion, amount of loose droppings, increases or mass gain, feed conversion and egg production in the case of poultry (Morales et al, 2011;Romero et al, 2009).…”

Section: Estimation Of Phytase Production By Ssfsupporting

confidence: 61%

“…The significance level of each variable was determined by Pareto charts, and statistical analyses of the data were carried out using analysis of variance (ANOVA) and Student´s t-test mean comparison test (p<0.05) (Statistica® 7, Statsoft, 217 Tulsa, OK, USA). The Box-Behnken design (BBD) was used to investigate the relationship between variables of medium components, and to optimize their concentrations for the best yield of phytase enzyme production in SSF, using A. niger (Bogar et al, 2003;Mittal et al, 2011). In experiments of the present study, four factors at three levels of variations were defined (Table 5).…”

Section: Experimental Design To Optimize the Medium Selected And Statmentioning

confidence: 99%

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Optimized production of phytase by solid-state fermentation using triticale as substrate and source of inducer

Neira-Vielma¹,

Cristobal²,

Anna³

et al. 2018

Afr. J. Biotechnol.

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This study was carried out to evaluate the process of phytase production by Aspergillus niger in solidstate fermentation (SSF) using triticale waste. A waste that currently has no use was reported for this biotechnological process, and is of high impact due to the null use. The process was carried out using an additive free medium, supplemented with only one nitrogen source. Under these conditions, phytase activity of 7.45 U/g dry substrate (DS) was obtained. The process was optimized using different additives such as dextrose, lactose, Tween 80 and potassium chloride. For fermentation maximization, two experimental designs were used: 1) Plackett-Burman design (PBD) and 2) the Box-Behnken design (BBD). PBD was used to evaluate the effect of related variables on the production of phytase, as well as their level of significance in the process, while BBD was used for optimal conditions determination. The process was conducted with Petri dishes and a maximum enzyme activity of 25.8 IU/g DS was obtained. Subsequently, SSF was carried out in a tray to increase the amount of fermented substrate and phytase activity of 23.63 IU/g DS was obtained. The results of this study suggest a minimal decrease (8.4%) in enzyme production with scaling.

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Section: Estimation Of Phytase Production By Ssfsupporting

confidence: 61%

Section: Experimental Design To Optimize the Medium Selected And Statmentioning

confidence: 99%

Optimized production of phytase by solid-state fermentation using triticale as substrate and source of inducer

Neira-Vielma¹,

Cristobal²,

Anna³

et al. 2018

Afr. J. Biotechnol.

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“…A. ficcum NRRL 3135 produced 25 U gds -1 using wheat bran as substrate (Bogar et al, 2003), and higher phytase yield (130 U.gds -1 ) was obtained using released meal by an A. niger A-98 locally isolated (El-Batal and Karem, 2001). …”

Section: Resultsmentioning

confidence: 99%

A simplified model for A. Niger FS3 growth during phytase formation in solid State fermentation

Spier

Letti

Woiciechowski

et al. 2009

Braz. arch. biol. technol.

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A simplified model to describe fungal growth during citric pulp fermentation for phytase production was described for the first time. Experimental data for biomass growth were adjusted to classical mathematical growth models (Monod and Logistic). The Monod model predictions showed good agreement with the experimental results for biomass concentration during 96 hours of fermentation. Parameters such as yield of biomass from oxygen (Y X/O ), maintenance coefficient (m) and specific growth rate (µ) were compared showing a good correlation between the data and the model. An alternative method for biomass determination in this process was developed since a great correlation was found between biomass growth and enzyme formation.

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“…Sedangkan A. niger menghasilkan enzim fitase paling tinggi setelah 3 hari inkubasi pada media yang mengandung 1 g/L gum, pada suhu 30°C (Papagianni et al, 2001). Aspergillus ficuum NRRL 3135, Mucor racemosus NRRL 1994 dan Rhizopus oligosporus NRRL 5905 menghasilkan enzim fitase tertinggi pada medium yang mengandung tepung canola, jagung, kedelai, dan dedak pada suhu 26 ± 1°C (Bogar et al, 2003). Hal tersebut menunjukkan suhu sekitar 30°C cukup optimal untuk memproduksi enzim fitase.…”

Section: Kondisi Optimum Produksi Fitase Pada Ssfunclassified

POTENSI DARI KAPANG Aspergilus niger, Rhizophus oryzae DAN Neurospora sitophila SEBAGAI PENGHASIL EZIM FITASE DAN AMILASE PADA SUBSTRATE AMPAS TAHU

Kanti

2017

BuletinPeternak

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INTISARIPenelitian ini bertujuan untuk mendapatkan kondisi optimal untuk produksi enzim amilase dan fitase pada media ampas tahu menggunakan Aspergillus niger (A. niger), Rhizophus oryzae (R. oryzae) dan Neurospora sitophila (N. sitophila) sebagai starter. Uji kemampuan produksi enzim fitase dan amilase oleh A. niger, R. oryzae dan N. sitophila dilakukan menggunakan media ampas tahu yang disterilisasi. Pemilihan ketiga isolat ini diawali dengan uji produksi enzim amilase pada kultur cair yang mengandung 2% pati, dan uji fitase dilakukan pada media yang mengandung 0,5% sodium fitat. Hasil uji pada medium cair selanjutnya digunakan untuk uji produksi enzim amilase dan fitase pada sistem fermentasi padat (SSF) menggunakan ampas tahu sebagai media fermentasi. Untuk mendapatkan produksi enzim yang tinggi dilakukan melalui optimasi waktu inkubasi, suhu inkubasi dan pH media. Fitase dan amilase dapat diproduksi dengan media ampas tahu oleh R. oryzae, A. niger dan N. sitophila. Kondisi optimum untuk produksi fitase, yaitu waktu inkubasi pada hari keempat untuk ketiga kapang, suhu 25°C untuk R. oryzae dan A. niger, suhu 30°C untuk N. sitophila, pH 8 untuk R. oryzae, pH 6 untuk A. niger dan N. Sitophila. Neurospora sitophila menghasilkan amilase optimum pada suhu 35°C, sedangkan A. niger dan R. oryzae optimum pada suhu 30°C. Aktivitas enzim amilase optimum adalah 162,23 U/g; 181,21 U/g dan 192,11 berturut turut oleh R. oryzae, A. niger dan N. Sitophila. Penurunan aktivitas produksi amilase menurun oleh R. oryzae pada suhu 40°C. Amilase diproduksi optimal pada pH 6-7. Aktivitas fitase yang dihasilkan oleh R. oryzae, A. niger dan N. sitophila, yaitu sebesar 51,32 U/g, 53,48 U/g, dan 60,54 U/g. Pakan ternak yang mengandung asam fitat mampu dihidrolisis oleh fitase pada kondisi optimum. Ketiga kapang juga menghasilkan enzim amilase pada media ampas tahu mengindikasikan bahwa ampas tahu merupakan susbtrat yang baik untuk produksi enzim hidrolisis yang berguna untuk meningkatkan nilai nutrisi pakan ternak.(Kata kunci: Amilase, Ampas tahu, Aspergillus niger, Neurospora sitophila, Phytase, Rhizopus oryzae) ABSTRACTThe objective of the study was to obtain the optimal condition to produce phytase and amylase enzyme using soybean curd residue with Aspergilus niger (A. niger) and Rhizopus oryzae (R. oryzae) and Neurospora sitophila (N. sitophila) as inoculants. These isolates were selected due to their ability to produce amylase in 2% of starch medium, and phytase in 0.5% of sodium phytase contain medium. Optimation of phytase and amylase production were performed on incubation time, pH, and temperature. Phytase and amylase were produced by these 3 isolates on soybean curd residue. Phytase production was achieved at 4 days incubation time. The optimal condition for phytase production varied depend on isolates. R. oryzae was optimal at pH 8, 25°C, while A. niger and N. sitophila were at pH 6.0-7.0 at 30°C. Amylase production was optimal at pH 6.0-7.0 after 3-4 days. Amylase production by R. oryzae was inhibited at 40°C. Application o...

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Optimization of phytase production by solid substrate fermentation

Cited by 83 publications

References 22 publications

Optimized production of phytase by solid-state fermentation using triticale as substrate and source of inducer

Optimized production of phytase by solid-state fermentation using triticale as substrate and source of inducer

A simplified model for A. Niger FS3 growth during phytase formation in solid State fermentation

POTENSI DARI KAPANG Aspergilus niger, Rhizophus oryzae DAN Neurospora sitophila SEBAGAI PENGHASIL EZIM FITASE DAN AMILASE PADA SUBSTRATE AMPAS TAHU

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