“…A significantly higher yield of cfDNA was obtained by QIAamp Circulating Nucleic Acid purification kit (Figure 15, p= 0.006570, one way ANOVA test). Blood was collected from six healthy volunteers in EDTA and Streck BCTs, and we observed no significant difference in cfDNA yield purified from plasma separated from Streck and EDTA tubes freshly, after 1 h, and 4 h. However, significantly higher cfDNA yield was observed in plasma samples purified from EDTA BCTs after 24 h of storage at 4°C, which could be due to gDNA contamination [158]. Comparison of centrifugation regimens revealed a slightly higher yield of cfDNA extracted from the plasma of blood samples centrifuged at 2000 x g at RT.…”