Optimization of preparative separation and purification of total polyphenols from Sargassum tenerrimum by column chromatography

Haider, Samee; Li, Zhenxing; Lin, Hong; Jamil, Kaiser

doi:10.1007/s11802-009-0425-x

Cited by 4 publications

(3 citation statements)

References 25 publications

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“…The PI3K-Akt signaling pathway is aberrantly activated during the occurrence and progression of certain cancers. The two most extensively elucidated mechanisms underlying PI3K-Akt activation in human cancer involve receptor tyrosine kinase stimulation and somatic mutations in specific components of signaling pathways ( 41 ). Augmentation and facilitation of the PI3K-Akt pathway may exert a detrimental impact on cancer therapy; hence, inhibition of PI3K could impede cancer development ( 42 ).…”

Section: Discussionmentioning

confidence: 99%

Metabolomics-transcriptomics joint analysis: unveiling the dysregulated cell death network and developing a diagnostic model for high-grade neuroblastoma

Zhang,

Sun

et al. 2024

Front. Immunol.

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High-grade neuroblastoma (HG-NB) exhibits a significantly diminished survival rate in comparison to low-grade neuroblastoma (LG-NB), primarily attributed to the mechanism of HG-NB is unclear and the lacking effective therapeutic targets and diagnostic model. Therefore, the current investigation aims to study the dysregulated network between HG-NB and LG-NB based on transcriptomics and metabolomics joint analysis. Meanwhile, a risk diagnostic model to distinguish HG-NB and LG-NB was also developed. Metabolomics analysis was conducted using plasma samples obtained from 48 HG-NB patients and 36 LG-NB patients. A total of 39 metabolites exhibited alterations, with 20 showing an increase and 19 displaying a decrease in HG-NB. Additionally, transcriptomics analysis was performed on NB tissue samples collected from 31 HG-NB patients and 20 LG-NB patients. Results showed that a significant alteration was observed in a total of 1,199 mRNAs in HG-NB, among which 893 were upregulated while the remaining 306 were downregulated. In particular, the joint analysis of both omics data revealed three aberrant pathways, namely the cAMP signaling pathway, PI3K-Akt signaling pathway, and TNF signaling pathway, which were found to be associated with cell death. Notably, a diagnostic model for HG-NB risk classification was developed based on the genes MGST1, SERPINE1, and ERBB3 with an area under the receiver operating characteristic curve of 0.915. In the validation set, the sensitivity and specificity were determined to be 75.0% and 80.0%, respectively.

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Section: Discussionmentioning

confidence: 99%

Metabolomics-transcriptomics joint analysis: unveiling the dysregulated cell death network and developing a diagnostic model for high-grade neuroblastoma

Zhang,

Sun

et al. 2024

Front. Immunol.

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“…Thus, SPE procedures, such as macroporous resins, silica gel and dispersants, are safer alternatives. Haider, Zhenxing, Hong, and Jamil (2009) demonstrated that macroporous resins are better than silica gel and polyvinylpolypyrrolidone in adsorbing/desorbing phlorotannins. Later, J.…”

Section: Phlorotannins Separationmentioning

confidence: 99%

Phlorotannins: From isolation and structural characterization, to the evaluation of their antidiabetic and anticancer potential

Erpel

Mateos

Pérez‐Jiménez

et al. 2020

Food Research International

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Glossary of Abbreviations iNOS: inducible nitric oxide synthase COX-2: cyclooxygenase 2 CAT: catalase SOD: superoxide dismutase GSH-px: glutathione peroxidase Bcl-2: -cell lymphoma 2 (anti-apoptotic protein of the Bcl-2 family) GLUT-4: glucose transporter type Fas: cell surface death receptor XIAP: X-linked inhibitor of apoptosis protein FLIP: FLICE (caspase 8)-inhibitory protein AKT: serine/threonine-specific protein kinase, also known as PKB (protein kinase B) Bid: BH3 interacting domain death agonist (pro-apoptotic protein of the Bcl-2 family) Bim: BH3 only protein (pro-apoptotic member of the Bcl-2 family) Bak: Bcl-2 homologous antagonist/killer (pro-apoptotic protein of the Bcl-2 family) Bax: Bcl-2-associated X protein (pro-apoptotic member of the Bcl-2 family) p53: tumor suppressor protein (p) 53 NF-B: nuclear factor kappa B, a transcription factor involved in stress responses and regulation of cell proliferation and apoptosis Bcl-xl: -cell lymphoma-extra large (anti-apoptotic protein of the Bcl-2 family) PI3K: phosphatidylinositol 3-kinase RAF-1: rapidly accelerated fibrosarcoma, a serine/threonine-specific kinase ERK: extracellular signal-regulated kinase EGFR: epidermal growth factor receptor, a proliferation-stimulating protein Con formato: Español (Chile) Con formato: Inglés (Estados Unidos) Con formato: Inglés (Reino Unido) According to Liu (2004), phytochemicals are categorized into six major groups: phenolic compounds, alkaloids, nitrogen-containing compounds, organosulfur compounds, phytosterols and carotenoids. According to Liu (2004), phytochemicals are bioactive non-nutritive compounds present in fruit, vegetables and other plant foods that have been related to reductions in the risks of major NCDs. They have been widely studied in the last two decades through in vitro assays, in vivo models and clinical trials, which have shed light into the structure-function relation responsible for their healthpromoting effects. In plants, phytochemicals accomplish defence and reproductive functions (Huang, Xiao, Burton-Freeman, & Edirisinghe, 2016). They are categorized into six major groups: phenolic compounds, alkaloids, nitrogen-containing compounds, organosulfur compounds, phytosterols and carotenoids. Phenolic compounds are the most studied phytochemicals since they are ubiquitous and abundant in all plant-based diets (Tsao, 2010). They are chemically defined as compounds having one or more aromatic rings with one or more hydroxyl groups (Liu, 2004). One high-value group of polyphenolic compounds-or polyphenols-only found in brown seaweeds are phlorotannins. These have attracted considerable interest because of their superior antioxidant capacity (Shibata, Ishimaru, Kawaguchi, Yoshikawa, & Hama, 2008; Wang et al., 2012) and valuable biological activities, such as anti-inflammatory, antihyperglycaemic and anti-tumour (Catarino, Silva, & Cardoso, 2017). Although phlorotannins have been widely studied through in vitro assays, in vivo models and some clinical trials, which have shed light into a possibl...

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“…The incidence and mortality rates of lung cancer are the highest among all cancer types, with these rates being the second highest among females [6]. Studies have shown that the polyphenols from green tea, apples, seaweed, and rice husks can help in inhibiting lung cancer in vitro or in vivo [7,8].…”

Section: Introductionmentioning

confidence: 99%

Polyphenols in Ilex latifolia Thunb. inhibit human lung cancer cell line A549 by regulation of the PI3K-Akt signaling pathway

Chen

Long

et al. 2022

BMC Complement Med Ther

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Background The leaves of the plant Ilex latifolia Thunb. can be made into Kuding tea, which is a drink rich in polyphenols. This study aimed to observe the effect of Ilex latifolia Thunb. polyphenols (ILTPs) on human lung cancer cell line A549 (A549 cells) by regulating the phosphatidylinositol 3-kinase/protein kinase B (PI3K/Akt) signaling pathway. Methods In vitro cultured cells were treated with ILTPs; the proliferation of A549 cells and BEAS-2B human normal lung epithelial cells (Beas-2B cells) was observed using the 3-(4,5-dimethylazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and the survival status of A549 cells was observed by fluorescence staining. The expression of A549 cells was observed by quantitative polymerase chain reaction (qPCR) assay and Western blot analysis, while the compound composition of ILTPs was detected using high-performance liquid chromatography (HPLC). Results The experimental results showed that the proliferation of Beas-2B cells was unaffected by treatment with 0–500 μg/mL of ILTPs, whereas the decreased proliferation of A549 cells was observed with the increasing concentrations of ILTPs. Additionally, ILTPs elevated the levels of lactate dehydrogenase (LDH) and reactive oxygen species (ROS) and promoted apoptosis in A549 cells. The results of qPCR experiments showed that ILTPs upregulated caspase-9 mRNA expression and downregulated phosphatidylinositol 3-kinase (PI3K), protein kinase B (Akt), mammalian target of rapamycin (mTOR), B-cell lymphoma-2 (Bcl-2), nuclear factor-κB (NF-κB), vascular endothelial growth factor (VEGF), hypoxia-inducible factor-1 alpha (HIF-1α), and cyclooxygenase-2 (COX-2) expression in A549 cells. The Western blot analysis results also showed that ILTPs could reduce the protein expression of PI3K and Akt. The HPLC results showed that the main compounds present in the ILTPs were rutin, kaempferol, isochlorogenic acid A, isochlorogenic acid B, and isochlorogenic acid C. Conclusions Thus, this study indicated that the polyphenols of I. latifolia act as a class of natural functional food materials that potently suppress cancer by exerting their inhibitory effects on A549 cell proliferation through five key polyphenolic compounds.

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Optimization of preparative separation and purification of total polyphenols from Sargassum tenerrimum by column chromatography

Cited by 4 publications

References 25 publications

Metabolomics-transcriptomics joint analysis: unveiling the dysregulated cell death network and developing a diagnostic model for high-grade neuroblastoma

Metabolomics-transcriptomics joint analysis: unveiling the dysregulated cell death network and developing a diagnostic model for high-grade neuroblastoma

Phlorotannins: From isolation and structural characterization, to the evaluation of their antidiabetic and anticancer potential

Polyphenols in Ilex latifolia Thunb. inhibit human lung cancer cell line A549 by regulation of the PI3K-Akt signaling pathway

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