Optimization of production, biochemical characterization and in vitro evaluation of the therapeutic potential of fibrinolytic enzymes from a new Bacillus amyloliquefaciens

Souza, Fabiana América Silva Dantas de; Sales, Aldo Torres; Silva, Pablo Eugênio Costa e; Bezerra, Raquel Pedrosa; Silva, Germana Michelle de Medeiros e; Araújo, Janete Magalí de; Campos-Takaki, Galba Maria de; Porto, Tatiana Souza; Teixeira, J. A.; Porto, Ana Lúcia Figueiredo

doi:10.1007/s13233-016-4089-2

Cited by 13 publications

(2 citation statements)

References 32 publications

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“…( A ) Bacillus sp. [ 32 , 49 , 50 , 74 , 75 , 76 , 77 , 78 , 79 , 80 , 81 , 82 , 83 , 84 ]; ( B ) Streptomyces sp. [ 36 , 85 , 86 , 87 , 88 , 89 , 90 , 91 ]; ( C ) other bacterial species [ 92 , 93 , 94 , 95 , 96 , 97 , 98 , 99 , 100 ].…”

Section: Microbes In Thrombolytic Therapymentioning

confidence: 99%

Thrombolytic Enzymes of Microbial Origin: A Review

Diwan

Usmani

Sharma

et al. 2021

IJMS

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Enzyme therapies are attracting significant attention as thrombolytic drugs during the current scenario owing to their great affinity, specificity, catalytic activity, and stability. Among various sources, the application of microbial-derived thrombolytic and fibrinolytic enzymes to prevent and treat vascular occlusion is promising due to their advantageous cost–benefit ratio and large-scale production. Thrombotic complications such as stroke, myocardial infarction, pulmonary embolism, deep venous thrombosis, and peripheral occlusive diseases resulting from blood vessel blockage are the major cause of poor prognosis and mortality. Given the ability of microbial thrombolytic enzymes to dissolve blood clots and prevent any adverse effects, their use as a potential thrombolytic therapy has attracted great interest. A better understanding of the hemostasis and fibrinolytic system may aid in improving the efficacy and safety of this treatment approach over classical thrombolytic agents. Here, we concisely discuss the physiological mechanism of thrombus formation, thrombo-, and fibrinolysis, thrombolytic and fibrinolytic agents isolated from bacteria, fungi, and algae along with their mode of action and the potential application of microbial enzymes in thrombosis therapy.

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Section: Microbes In Thrombolytic Therapymentioning

confidence: 99%

Thrombolytic Enzymes of Microbial Origin: A Review

Diwan

Usmani

Sharma

et al. 2021

IJMS

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“…Bacillus amyloliquefaciens UFPEDA 485 produced fibrinolytic protease that exhibited long-term stability, and activity at physiological conditions, and could be of therapeutic importance for human and veterinary applications (de Souza et al 2016). Streptomyces sp.…”

Section: Fibrinolytic Proteases As Thrombolytic Agentsmentioning

confidence: 99%

Potential application spectrum of microbial proteases for clean and green industrial production

Singh

Bajaj

2017

Energ. Ecol. Environ.

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Enzymes are the cornerstones of metabolism and constitute the fundamental basis for existence of life. However, recently enzymes are being implicated in diverse industrial processes because of their specific and fast action for efficient bioconversion of substrate to product, and their capability to save raw materials, energy and chemicals for various manufacturing processes. Enzymes are considered as environment-friendly (green) chemicals that may potentially help replacing completely or reducing the usage of hazardous chemicals for industrial processes, thus promising sustainable production and manufacturing. Among various industrial enzymes microbial proteases dominate the world enzyme market due to their multifaceted application potential in varied bioindustries like food, pharmaceutical, textile, photographic, leather and detergent. Promising applications of proteases in agricultural sector for instance may include biocontrol of pests, degumming of silk, selective delignification of hemp and wool processing. However, for successful industrial applications the proteases must be robust enough to suit the process conditions which are generally hostile. Proteases intended for industrial applications must have activity and stability over wide range of temperature and pH extremes for prolonged time periods and even in the presence of various potential enzyme inhibitors. Of various microbial proteases those from Bacillus spp. have got special significance because the latter are known for their ability to produce sturdy enzymes that might have suitability for industrial process conditions. The current article presents an interpretive summary of the recent developments on application potential of proteases for various industries.

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Fibrinolytic protease fromBacillus cereusS46: Purification, characterization, and evaluation of its in vitro thrombolytic potential

2020

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Intravascular thrombosis is a prime cause of cardiac complications worldwide. Microbial fibrinolytic proteases are of clinical significance in thrombosis treatment. The present study discusses the purification and characterization of a protease from Bacillus cereus S46, ascertaining its in vitro thrombolytic activity against a blood clot. By the three‐step purification involving precipitation, dialysis, and diethylaminoethyl‐cellulose ion‐exchange chromatography, a 12.37‐fold purification of the enzyme to homogeneity was achieved. The apparent molecular mass of the protease was 30 kDa, as found by sodium dodecyl sulfate–polyacrylamide gel electrophoresis. The optimum activity of the enzyme was observed at pH 8.0 and 40°C. The enzyme retained an 82.19% residual activity at pH 8.0 and 40°C for 1 h. The Km and Vmax values of the protease with casein were 0.0027 mM and 9.712 µmol/min, respectively. In an in vitro assay, the purified protease resulted in 97.02% lysis of the blood clot. The fibrinolytic potential of the enzyme, together with its characteristics of being active and stable under near‐physiological conditions, may suggest its application as a therapeutic agent.

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Optimization of production, biochemical characterization and in vitro evaluation of the therapeutic potential of fibrinolytic enzymes from a new Bacillus amyloliquefaciens

Cited by 13 publications

References 32 publications

Thrombolytic Enzymes of Microbial Origin: A Review

Thrombolytic Enzymes of Microbial Origin: A Review

Potential application spectrum of microbial proteases for clean and green industrial production

Fibrinolytic protease fromBacillus cereusS46: Purification, characterization, and evaluation of its in vitro thrombolytic potential

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