Optimization of production of docosahexaenoic acid (DHA) by<i>Thraustochytrium aureum</i> ATCC 34304

Bajpai, Pratima; Ward, Owen P.

doi:10.1007/bf02663823

Cited by 143 publications

(40 citation statements)

References 34 publications

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“…The strains C36 and C22 for their high DHA concentrations, which were higher than what is described in the literature (Bajpai et al 1991, Burja et al 2006, Yamasaki et al 2006, Chi et al 2007. Rhodosporidium toruloides and Rhodotorula glutinis produce around 70 and 33.7% of total lipids in dry weight, respectively.…”

Section: Discussionmentioning

confidence: 76%

Marine fungoid producers of DHA, EPA and carotenoids from central and southern Chilean marine ecosystems

Pino¹,

Socias²,

González³

2015

Rev. biol. mar. oceanogr.

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Resumen.-La creciente demanda de ácidos grasos poliinsaturados (AGPI) provocada por la escasez de pesca extractiva, ha impulsado la obtención de fuentes alternativas sustentables para abastecer su mercado. Los Thraus tochytridos y levaduras marinas (fungoides marinos), son potenciales productores de lípidos y carotenoides de importancia comercial. Desde una colección de 41 cepas de fungoides marinos aisladas en el Sistema de Corriente de Humboldt, se determinó la capacidad de producir DHA (ácido docosahexanoico), EPA (ácido eicosapentanoico) y CT (carotenoides totales) en cultivos líquidos comercial (MS) y alternativos (PDB, GAM, BAM y MCM). Los resultados indican que el medio MS y PDB, presentaron las mayores velocidades de crecimiento (0,02 h -1 ). No obstante, el medio MS presenta estos parámetros cinéticos a 16ºC y el PDB a 37°C. Trece de las cepas analizadas presentaron una alta capacidad para producir DHA (hasta 23% peso seco) y CT (hasta 18% peso seco), comparables a los niveles observados en Schizochytrium sp. KH105 y Rhodosporidium toruloides. Adicionalmente, todas las cepas ensayadas también produjeron pequeñas cantidades de EPA (hasta 0,3% peso seco). La microscopia electrónica revela que la cepa C36 es morfológicamente consistente con levaduras y su secuencia parcial del gen ribosomal 18s presenta un 97% de similitud con el género Rhodotorula, el que hasta ahora no ha sido reportado como productor de DHA y EPA. Finalmente, las cepas C36, C22 y C4 son interesantes objetos de estudio para escalar su producción y proyectar su uso comercial, como por ejemplo para suplementar en omega-3 y carotenoides alimentos para consumo humano, animales de corral o para estadios larvales de peces cultivados y pigmentar la carne del salmón, entre otros.Palabras clave: Fungoides marinos, DHA, EPA, carotenoides totales, Rhodotorula, parámetros cinéticos Abstract.-Declining fishing yields have pushed the search for sustainable alternative sources for polyunsatura ted fatty acids (PUFAs). Thraustochytrids and marine yeasts (marine fungoid protists) are potential commercial sources of lipids and carotenoids. It was determined the capacity of a collection of 41 strains of marine fungoid isol ated in the Humboldt Current System, to produce DHA (docosahexanoic acid), EPA (eicosapentanoic acid) and CT (total carotenoids) in commercial growing media (MS) and alternative growing mediums (PDB, GAM, BAM and MCM). The media MS and PDB exhibited the highest growth rate (0.02 h -1 ), at 16 and 37°C, respectively. Thirteen of the studied strains showed high capacity to produce DHA (up to 23% dry weight) and CT (up to 18% dry weight), comparable to levels observed in Schizochytrium sp. KH105 and Rhodosporidium toruloides. Additionally, all studied strains produce small amounts of EPA (up to 0.3% of dry weight). Scanning electron microscopy reveals that strain C36 is morphologically consistent with yeasts, while partial sequencing of the 18s ribosomal gene shows 97% similarity to the genus Rhodotorula, which has not been reported until n...

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Section: Discussionmentioning

confidence: 76%

Marine fungoid producers of DHA, EPA and carotenoids from central and southern Chilean marine ecosystems

Pino¹,

Socias²,

González³

2015

Rev. biol. mar. oceanogr.

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“…On the contrary, it is also known that some other phytoplankton and marine fungi, such as Dinophyta and Chromista, contain notable levels of DHA more than 3 of TFA Dinoflagellida [34][35][36] and Labyrinthulida [37][38][39] . The signifi cant levels of DHA in C.…”

Section: Fatty Acid Composition In Depot Tag In C Gigas and Relationmentioning

confidence: 99%

High Levels of Icosapentaenoic Acid in the Lipids of Oyster Crassostrea gigas Ranging over Both Japan and France

Saito¹,

Marty²

2010

J. Oleo Sci.

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The lipid and fatty acids of the Pacific oyster, Crassostrea gigas, collected both in Japan and France, were analyzed. Triacylglycerols and sterols were the major classes in the neutral lipids of both populations of C. gigas between the two countries, and they had signifi cant levels of phospholipids (phosphatidylethanolamine and phosphatidylcholine) with ceramideaminoethylphosphonate in their polar tissue lipids. The differences of 18:3n-3 and 22:6n-3 (docosahexaenoic acid; DHA) levels in the depot triacylglycerols suggest the infl uence of dietary phytoplankton in different environments of the two countries. Although the levels of the fatty acids slightly differed, that of 20:5n-3 (icosapentaenoic acid; EPA, 20.5-24.8%) in the triacylglycerols was specifi cally the highest fatty acid in all the samples. The major polyunsaturated fatty acids in the polar lipids of all samples were EPA (13.2-17.8% for phosphatidylethanolamine and 13.4-22.7% for phosphatidylcholine) and DHA (18.9-26.8% for phosphatidylethanolamine and 13.1-22.5% for phosphatidylcholine). The fl uctuation of total PUFA levels in the polar lipids between samples of both countries suggests the infl uence of diet. In addition, the consistently high EPA levels in the phospholipids may compensate for the variation in DHA levels.

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“…A similar assimilation profile of carbon sources has been observed for A. limacinum SR21 8,22) . Comparative results were reported for S. aggregatum and T. aureum, which could utilize maltose and starch as carbon sources 23,24) . Carbon source utilization profiles could therefore be useful for the identification and classification of thraustochytrid species.…”

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confidence: 99%

Optimization of Culture Conditions for Growth and Docosahexaenoic Acid Production by a Marine Thraustochytrid, Aurantiochytrium limacinum mh0186

et al. 2009

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Optimization of production of docosahexaenoic acid (DHA) byThraustochytrium aureum ATCC 34304

Cited by 143 publications

References 34 publications

Marine fungoid producers of DHA, EPA and carotenoids from central and southern Chilean marine ecosystems

Marine fungoid producers of DHA, EPA and carotenoids from central and southern Chilean marine ecosystems

High Levels of Icosapentaenoic Acid in the Lipids of Oyster Crassostrea gigas Ranging over Both Japan and France

Optimization of Culture Conditions for Growth and Docosahexaenoic Acid Production by a Marine Thraustochytrid, Aurantiochytrium limacinum mh0186

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