Optimization of RAPD‐PCR conditions in cattle

Ambady, Sakthikumar; Carpio, C. M.; León, F. A. Ponce de

doi:10.1080/10495399609525851

Cited by 3 publications

(2 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The RAPD-PCR conditions to get reproducible banding pattern varies between laboratories (Penner et al, 1993;Rothuizen and Wolferen, 1994;Ambady et al, 1996;Parejo et al, 1997;Yoon and Kim, 2004). Standardization of RAPD-PCR conditions was done under this study so that reproducible fingerprints were obtained.…”

Section: Resultsmentioning

confidence: 99%

Assessment of Bandsharing Values in RAPD-PCR Analysis of Dwarf Cattle of Kerala

Suprabha¹,

Anilkumar²,

Aravindakshan³

et al. 2005

Asian Australas. J. Anim. Sci

View full text Add to dashboard Cite

Random amplified polymorphic DNA (RAPD-PCR) analysis of 56 animals of four different genetic groups of dwarf cattle in Kerala was done as a single step analysis. Bandsharing (BS) values were calculated for animals of each group and between groups as an analytical tool to find out genetic variation among animals. The different factors affecting BS values were estimated using Harvey's Least squares analysis. The effects of genetic group, Guanine-cytosine (GC) content of primer and gel on BS values were found significant. Bandsharing values of Kasargode-Highrange dwarf animals were significantly different from Vechur, Vatakara and their combinations. The Vechur, Vatakara and Vechur-Vatakara combinations were found to be more uniform (high BS value) compared with other combinations. The bandsharing value was lowest with primers of GC content 90% and highest with 80% GC content. The effect of gel on BS value points to the need of adjustments of gel factor for calculation of BS values.

show abstract

Section: Resultsmentioning

confidence: 99%

Assessment of Bandsharing Values in RAPD-PCR Analysis of Dwarf Cattle of Kerala

Suprabha¹,

Anilkumar²,

Aravindakshan³

et al. 2005

Asian Australas. J. Anim. Sci

View full text Add to dashboard Cite

show abstract

“…Therefore, it is important to identify as many RAPD bands as possible. In a study on Holstein progeny test bulls, primers with 10 bp in length and 60-70% G+C content were used and produced 4-10 bands (6). In this study, ten bases in length and 50-80% G+C content were used, yielding 4 to 15 RAPD bands per primer.…”

Section: Discussionmentioning

confidence: 99%

Use of RAPD-PCR for genetic analyses on the native cattle breeds in Turkey

Güven;AKYÜZ¹

2010

Ankara Üniversitesi Veteriner Fakültesi Dergisi

View full text Add to dashboard Cite

Summary:The purpose of this study was to identify the genetic relationship and variations within and between local cattle breeds of Turkey using RAPD-PCR method which needs fewer substructures when compared to other DNA fingerprinting methods. The RAPD-PCR method was performed on blood samples of 77 animals from four breeds namely Anatolian Black (AB), East Anatolian Red (EAR), South Anatolian Red (SAR) and Turkish Grey Steppe (TG). A set of ten arbitrary 10-mer primers were used to detect the DNA polymorphisms in the whole genome. Estimation of the genetic distances between the breeds was conducted through the F ST Distant Estimation Method, and cluster trees were constructed by UPGMA Method. Based on the band sharing method, the average genetic variances of the breeds varied from 0.637 (in TG) to 0.809 (in SAR), AB and EAR had 0.792 and 0.787, respectively. It was concluded that AB breed is more closely related to EAR breed than to SAR breed while TG breed was determined as a distinct group from other breeds.

show abstract

Random amplified polymorphic DNA (RAPD) sequences as markers for canine genetic studies

Olivier

Meehl

Lust

1999

Journal of Heredity

View full text Add to dashboard Cite

Random amplified polymorphic DNA (RAPD) markers have been used in genetic studies of several plant and animal species. However, concerns exist about the reproducibility of RAPD-PCR reactions. Therefore, the use of specific 24mer primer pairs in standard PCR reactions has been suggested for reliable amplification of characterized polymorphic RAPD sequences. The purpose of this article is describe the application of the RAPD-PCR assay to genetic studies of dogs and to investigate the amplification of RAPD sequences with specific primer pairs. Of 240 decanucleotide primers tested by PCR, 34.6% resulted in amplification of at least one polymorphic fragment with samples of a Labrador retriever pedigree. We cloned and sequenced five of these RAPD fragments and synthesized specific 24mer primer pairs for each. Two primer pairs amplified a sequence exclusively from samples that were positive for the RAPD fragment, while three others amplified the respective sequence from all DNA samples. A new polymorphism was observed in the restriction digest products with Msel of one of the amplification products. None of the cloned sequences contains an open reading frame longer than 213 bases. Two sequences hybridized only to specific fragments of genomic DNA from samples that amplified the RAPD, the remaining three sequences hybridized to multiple sequences in all canine samples tested by Southern analysis. None of the five fragments hybridized to human or murine genomic DNA. Data suggested that RAPD sequences can be used as molecular markers in genetic studies of diseases in dogs. However, the use of specific primer pairs leads to loss of the RAPD polymorphism in three of five sequences tested.

show abstract

Optimization of RAPD‐PCR conditions in cattle

Cited by 3 publications

References 12 publications

Assessment of Bandsharing Values in RAPD-PCR Analysis of Dwarf Cattle of Kerala

Assessment of Bandsharing Values in RAPD-PCR Analysis of Dwarf Cattle of Kerala

Use of RAPD-PCR for genetic analyses on the native cattle breeds in Turkey

Random amplified polymorphic DNA (RAPD) sequences as markers for canine genetic studies

Contact Info

Product

Resources

About