Optimization of reaction parameters of acidolysis reaction between mustard oil and capric acid by using Thermomyces lanuginosus lipase

Abstract

Structured lipids were prepared from mustard oil by enzymatic acidolysis reaction with capric acid (C10) using lipase enzyme TLIM from Thermomyces lanuginosus as biocatalyst. Parameters such as substrate molar ratio, enzyme concentration, reaction temperature, stirring speed and time of maximum incorporation, were studied for the optimization of the reaction. The optimized set of process conditions was predicted by response surface methodology (RSM) and genetic algorithm (GA). The robustness of GA and RSM was evaluated using regression coefficient and p value. The R(2) found out by GA was 0.996 while from RSM was 0.973. The results proved that GA models have better performance than RSM models. From the result, it could be concluded that optimal conditions for synthesis of capric acid rich mustard oil were: Temperature = 39.5 °C ; time = 21.1 hr; Substrate ratio = 3.5; Enzyme content = 8.8%; Speed = 570.8 rpm.

Lipase-Catalyzed Interesterification for the Synthesis of Medium-Long-Medium (MLM) Structured Lipids

“…Khodadadi et al ( 43 ) also reported that Lipozyme TL IM and Novozyme 435 were more effective in transesterification reaction between flaxseed oil and tricaprylin than Lipozyme RM IM and Amano DF. Table 1 ( 44 , 48 - 53 ) summarizes the utilization of immobilized lipase for MLM lipase synthesis.…”

Recent Research Trends on the Enzymatic Synthesis of Structured Lipids

“…Many of recent studies have produced MLM‐type MLCTs from diverse kinds of plant oils (mainly soybean, corn, olive, canola, rice bran, avocado, mustard, borage, echium, and pine nut oils and palm olein) and MCFAs, such as caprylic (8:0) and capric acids (10:0). These published studies employed free or immobilized sn ‐1,3‐specific lipases developed in laboratories of Kim and others (), Nunes and others (), Ifeduba and Akoh (), and Qin and others (), as well as commercial immobilized sn ‐1,3‐specific lipases, such as Lipozyme RM IM and Lipozyme TL IM (Jennings and others ; Ozturk and others ; Sengupta and Ghosh ; Chnadhapuram and Sunkireddy ; Choi and others ; Savaghebi and others ; Wang and others ; Gokce and others ; Caballero and others ; Silroy and others ). In particular, the use of PBRs is preferred in the enzymatic preparation of MLM‐type MLCTs because rupture of the supporting materials for immobilized lipases, which facilitates acyl migration that causes the formation of other types of MLCT species can be avoided in these reactors (Xu ).…”

Production of Medium Chain Fatty Acid Rich Mustard Oil Using Packed Bed Bioreactor

“…Batch Reactor STBR MCFA capric, caprylic and lauric and mustard oil were taken in 3:1 molar ratio and stirred at 200 rpm at 60 for different time periods with 10 of three different lipozyme enzymes 2 . The total volume of the substrate was 100 ml.…”

“…Medium chain fatty acids MCFA rich mustard oil was prepared in our previous study by enzymatic acidolysis reaction using Thermomyces lanuginosus lipase as biocatalyst 2 . We thought about MCFA because triglycerides containing MCFA ie.…”