Optimization of selective production media for enhanced production of xylanases in submerged fermentation by Thielaviopsis basicola MTCC 1467 using L16 orthogonal array

Abstract

Enzymes have been the centre of attention for researchers/industrialists worldwide due to their wide range of physiological, analytical, food/feed and industrial based applications. Among the enzymes explored for industrial applications, xylanases play an instrumental role in food/feed, textile/detergent, paper and biorefinery based application sectors. This study deals with the statistical optimization of xylanase production by Thielaviopsis basicola MTCC 1467 under submerged fermentation conditions using rice straw, as sole carbon source. Different fermentation parameters such as carbon source, nitrogen source, inorganic salts like KH 2 PO 4 , MgSO 4 and pH of the medium were optimized at the individual and interactive level by Taguchi orthogonal array methodology (L 16

Optimization of the production of xylanases in corncob-based media by Aspergillus niger and Trichoderma longibrachiatum using Taguchi approach

“…The results indicated a total significant increment of 208.09 and 192.59% when compared with the conventional unoptimized xylanase synthesis by both A. niger L3 and T. longibrachiatum L2, respectively. These results compared well with previous reports of xylanase production that were optimized using Taguchi method where 277%, 161.5%, 41.9 and 10.24% higher enzyme yields were obtained (Lakshmi et al 2009;Rani et al 2014;Mandal et al 2015). The increase can be claimed to have resulted from the interactions and relations among the various factors (Mandal et al 2015).…”

“…Such carbon source or substrate concentration dependent enzyme production has been reported in diverse xylanase synthesizing microbial strains (Oliveira et al 2006;Kapoor et al 2008). Also, Rani et al (2014) reported that carbon source (rice straw) produced the greatest effect in the production of xylanase.…”

“…Understanding of the influence or effect of each individual factor is crucial for the implementation of a successful bioprocess operation (Rani et al 2014). Therefore, ANOVA was employed in evaluating results of the orthogonal array experiment and to study the level of consequence of the fermentation parameters on the deviation of the responses (Lakshmi et al 2009).…”

“…Its use most recently reported for the green synthesis of nanoparticles with profound biomedical applications (Elegbede et al 2018a(Elegbede et al , 2018b(Elegbede et al , 2019 has further expanded the frontiers of enzyme technology in nanobiotechnology (Lateef and Adeeyo 2015;Adelere and Lateef 2016). Broad types of fungi and bacteria are known to synthesize xylanases under various cultivation systems, but filamentous fungi are the most effective producers of xylanase, because they ARTICLE Optimization of the production of xylanases in corncob-based media by Aspergillus niger and Trichoderma longibrachiatum using Taguchi approach secrete elevated levels of enzymes compared to yeasts and bacteria (Rani et al 2014). On an industrial scale, xylanases are principally synthesized by Aspergillus and Trichoderma spp.…”

“…Optimization of production of xylanase is a prerequisite for its large-scale economical production (Azin et al 2007), and this can be achieved by manipulating important parameters that affect the fermentation process (Rani et al 2014). Production of xylanase differs in diverse strains and this can be regulated by the nutritional, physiological, and biochemical nature of microbes that are employed (Lakshmi et al 2009).…”