Utilization of Corn Cob Waste for Cellulase-Free Xylanase Production by Aspergillus niger DX-23: Medium Optimization and Strain Improvement

Desai, Dhara; Iyer, Bragadish D

doi:10.1007/s12649-016-9567-4

Cited by 13 publications

(2 citation statements)

References 44 publications

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“…niger is a commonly found strain of fungi belonging to the lamentous fungi genus Aspergillus and is widely used in the industrial enzyme preparation eld due to its high yield, wide application range, and good safety. The enzyme preparations produced with A. niger include more than 30 different types of enzymes, such as amylase [9] , starch enzyme [10] , tannase [11] , cellulase [12] , hemicellulose [13] , and pectinase [14] . In addition, A. niger has received increasing attention in the liquefaction, alcohol, and glucose manufacturing industries.…”

Section: Introductionmentioning

confidence: 99%

Biological detoxification and metabolism of inhibitors in corncob acid hydrolysate using Aspergillus niger

Yin,

Wang,

et al. 2023

Preprint

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Background The complex structure of lignocellulosic raw materials requires pretreatment and enzymatic hydrolysis to convert them into monosaccharides for further microbial utilization. During pretreatment, the main inhibitory compounds produced are acetic acid, furfural, and 5-hydroxymethylfurfural (HMF), which are significant factors limiting the microbial growth and fermentation of lignocellulosic materials. Biological detoxification is an efficient, gentle, and environmentally friendly method for removing fermentation inhibitors in lignocellulose hydrolysate. Results Aspergillus niger (A. niger) M13 efficiently metabolizes acetic acid, furfural, and HMF at concentrations of at least 7.50, 1.81, and 1.02 g/L, respectively. Among these inhibitors, furfural exerts the strongest inhibitory effect on cell growth. Both M13 spores and mycelial balls can completely remove the major inhibitors in the hydrolysate, with spores exhibiting a higher removal efficiency than mycelial balls. The detoxification rates of acetic acid, furfural, and HMF by M13 spores were 0.1566, 0.1125, and 0.015 g/L/h, respectively. This strain prefered to consume furfural first, followed by HMF, and then simultaneously consumes acetic acid and glucose. Finally, M13 spores can produce a small amount of citric acid (about 6 g/L) directly from the non-detoxified hydrolysate. Conclusions Using A. niger M13 spores for detoxification is a highly feasible option. These spores can completely remove the main inhibitory substance from the hydrolysate within 1–2 days while retaining the carbon source for subsequent fermentation production. Biodetoxification by A. niger M13 provided a fast and efficient biodetoxification method for removing inhibitors generated during intensive lignocellulose pretreatment, and its advantages made it possible for potential industrial application.

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Section: Introductionmentioning

confidence: 99%

Biological detoxification and metabolism of inhibitors in corncob acid hydrolysate using Aspergillus niger

Yin,

Wang,

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…niger is a commonly found strain of fungi belonging to the filamentous fungi genus Aspergillus, and it is widely used in the industrial enzyme preparation field due to its high yield, wide application range, and good safety. The enzyme preparations produced with A. niger include more than 30 different types of enzymes, such as amylase [13,14], starch enzyme [15], tannase [16][17][18], cellulase [19,20], hemicellulose [21,22], and pectinase [23]. In addition, A. niger has played a significant role in industrial fermentation and has received increasing attention in the liquefaction, alcohol, and glucose manufacturing industries [24].…”

Section: Introductionmentioning

confidence: 99%

Biological Detoxification of the Inhibitors in Corncob Acid Hydrolysate Using Aspergillus niger

Yin,

Wang,

et al. 2023

Fermentation

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The biological detoxification of lignocellulose hydrolysate is an effective method through which to enhance microbial fermentation efficiency. In this study, an inhibitor-tolerant strain of A. niger (Aspergillus niger) was used for the biological detoxification of corncob hydrolysate. The results showed that A. niger M13 can tolerate a concentration of at least 7.50 ± 0.19 g/L of acetic acid, 1.81 ± 0.13 g/L of furfural, and 1.02 ± 0.10 g/L of HMF (5-Hydroxymethylfurfural). The spores had a higher detoxification efficiency than the mycelial pellets with a detoxification rate of 0.1566 g/L/h, 0.1125 g/L/h, and 0.015 g/L/h for acetic acid, furfural, and HMF, respectively. The cell preferentially consumed furfural, then HMF, before simultaneously degrading acetic acid and glucose. A. niger M13 spores could accumulate small amounts of citric acid directly from undetoxified hydrolysate at a concentration of about 6 g/L. Therefore, A. niger M13 can serve as an excellent biological detoxification strain and a potential citric acid fermenting strain when using undetoxified lignocellulosic hydrolysates.

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Optimization of Xylanase Production by Trichoderma orientalis Using Corn Cobs and Wheat Bran via Statistical Strategy

Long

Liu

Gan

et al. 2017

Waste Biomass Valor

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Utilization of Corn Cob Waste for Cellulase-Free Xylanase Production by Aspergillus niger DX-23: Medium Optimization and Strain Improvement

Cited by 13 publications

References 44 publications

Biological detoxification and metabolism of inhibitors in corncob acid hydrolysate using Aspergillus niger

Biological detoxification and metabolism of inhibitors in corncob acid hydrolysate using Aspergillus niger

Biological Detoxification of the Inhibitors in Corncob Acid Hydrolysate Using Aspergillus niger

Optimization of Xylanase Production by Trichoderma orientalis Using Corn Cobs and Wheat Bran via Statistical Strategy

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