Optimization of Solid-State Fermentation for Improved Conidia Production ofBeauveria bassianaas a Mycoinsecticide

Abstract

The production of conidia of entomopathogenic Beauveria bassiana by solid-state fermentation was studied for the development of a biocontrol agent against aphid Myzus persicae. The optimal conditions for conidia production on polished white rice were 40% moisture content, 25℃ culture temperature, 2-day-old seeding culture grown in 3% corn meal, 2% rice bran, 2% corn steep powder medium, initial conidia concentration of 107 conidia/g in the wet rice, 10% inoculum size, and use of a polyethylene bag as a container. The polyethylene bag containing inoculated rice was hand-shaken every 12 hr during fermentation. Using optimal conditions, the maximum conidia production obtained was 4.05 g conidia/100 g dry rice after 14 days of cultivation, a rate 2.83 times higher than conidia yield of pre-optimization.

Challenges of Enzymes, Conidia and 6-Pentyl-alpha-pyrone Production from Solid-State-Fermentation of Agroindustrial Wastes Using Experimental Design and T. asperellum Strains

“…Table 6 shows the C/N ratio of the different solid medium tested. Among medium tested, the mixture containing 30% of vine shoots, 20% of potatoes flour, 20% of jatropha, 20% of olive pomace and 10% of olive oil (medium 1) presents the optimal C/N ratio (37). From these results, it is evident that C/N ratio correlates with metabolites production because this mixture is the optimal medium defined for T. asperellum TV104 to produce 6-PP, conidia and enzymes.…”

Morphological characterization and optimization of conditions for conidial production of Elsinoë ampelina, the causal organism of grapevine anthracnose

“…Aeration during cultivation is important for conidia production as well. For example, aeration significantly improved the conidia production of Beauveria bassiana (Kang et al., ; Pham et al., ). Therefore, a normal atmosphere probably has a positive effect on conidial formation for E. ampelina , whereas low oxygen strikes an oxidant–antioxidant balance and causes a negative effect.…”

“…Aeration during cultivation is important for conidia production as well. For example, aeration significantly improved the conidia production of Beauveria bassiana (Kang et al, 2005;Pham et al, 2010).…”

“…However, the density of colonies is difficult to control. Several reports showed that aeration significantly improves conidial production (Kang, Lee, Yoon, & Kim, 2005;Pham, Kim, & Kim, 2010). Others reported that conidial formation is more abundant at low than at high temperatures (Houston & Oswald, 1946).…”

Characterization of a mutant strain of a filamentous fungus Cladosporium phlei for the mass production of the secondary metabolite phleichrome

“…We obtained a total of 592 mg of phleichrome from 25 plates produced by pouring 40 ml of melted V8 juice agar. The phleichrome production was around 146 mg and 446 mg from mycelia and agar medium, respectively, suggesting that solid instead of liquid fermentation is the preferred culture condition for the mass production of phleichrome (Pham et al, 2010) (Table 1). These results indicate that UV-mutagenesis followed by visual inspection and rapid TLC analysis using agar blocks is a successful method for selecting a mutant strain that overproduces phleichrome.…”