Optimization of some fermentation conditions for the production of extracellular amylases by using Chryseobacterium and Bacillus isolates from organic kitchen wastes

Hasan, Md. Mahbub; Marzan, Lolo Wal; Hosna, Asamul; Hakim, Al; Azad, Abul Kalam

doi:10.1016/j.jgeb.2017.02.009

Cited by 51 publications

(44 citation statements)

References 29 publications

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“…Similar observations were reported by Hasan et al (2017) too. The reduction in amylase activity after altering the pH of the culture medium might be due to the denaturation or inactivation of proteins.…”

Section: Discussionsupporting

confidence: 91%

“…The availability of essential elements, coenzymes, nitrogenous constituents, and growth factors in yeast extract might be responsible for the amylase inducing property of the isolate. However, Hasan et al (2017) revealed peptone as the most stimulatory nitrogen source for maximum production of amylases from both Chryseobacterium sp. and Bacillus sp.…”

Section: Discussionmentioning

confidence: 99%

“…(Hasan et al 2017). In the line of those reports, we had undertaken a further significant attempt towards the amylolytic research, and isolated a new strain of G. arilaitensis with hyperamylase producing potentiality.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, few investigations had already reported the overwhelming yield of amylases from distinct bacterial sources using unique organic wastes viz. cassava bagasse (Ray and Kar 2009), cow dung (Vijayaraghavan et al 2015), potato starchy wastes (Abd-Elhalem et al 2015), and organic chicken wastes (Hasan et al 2017). But study on the utilization of goat dung as feedstock for the successful production of amylase is not evidenced so far.…”

Section: Discussionmentioning

confidence: 99%

“…In recent years, amylases have been screened and characterized from novel strains of bacteria including Bacillus cereus (Roohi et al 2013), Bacillus thuringiensis (Smitha et al 2013), Aeromonas veroni and Stenotrophomonas maltophilia (Kumar et al 2014), and Chryseobacterium sp. (Hasan et al 2017).…”

Section: Introductionmentioning

confidence: 99%

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Goat dung as a feedstock for hyper-production of amylase from Glutamicibacter arilaitensis strain ALA4

Aarti¹,

Khusro²,

Agastian³

2017

Bioresour. Bioprocess.

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Background:In modern times, bacteria-associated products; especially enzymes are gaining immense interest among worldwide researchers. Among several enzymes, amylases are of great significance in bioprocess engineering. This investigation was aimed to optimize the amylase production from Glutamicibacter arilaitensis strain ALA4 using goat dung as an inexpensive substrate in solid-state fermentation.Results: Amylase production was initially improved by optimizing physical factors and medium components by one factor at a time method. Two-level full factorial design (2 5 factorial matrix) was applied to screen the selected variables using first-order polynomial model. Parameters such as incubation temperature, moisture, starch, and yeast extract affected the amylase activity significantly (P < 0.05). Central composite design at N = 30 was further employed to evaluate the optimum levels of these variables by a second-order polynomial equation. Maximal amylase activity of 4572.53 ± 41.71 U/g was estimated in the goat dung medium supplemented with 100% moisture, 1% (w/w) starch, and 1% (w/w) yeast extract, being incubated at 40 °C. The optimized parameters revealed approximately twofold increment in the amylase yield (R 2 0.9169) with respect to the original medium. The amylase showed stability at high pH and temperature up to 4 h of incubation with residual activities of 52.32 ± 2.2 and 46.12 ± 3.3%, respectively. Additionally, the enzyme revealed resistant property not only towards various metal ions and organic solvents but also surfactants and inhibitors. Most importantly, the amylases obtained from strain ALA4 depicted remarkable tolerance to commercially available various detergents. Conclusions:This study reports first reference on the hyper-production of amylase from G. arilaitensis using goat dung as the low-cost agro-waste medium. G. arilaitensis strain ALA4 may be utilized for wide spread applications in several bioprocess industries due to the high stability of its amylase towards diversified pH, temperature, solvents, surfactants, and detergents.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Goat dung as a feedstock for hyper-production of amylase from Glutamicibacter arilaitensis strain ALA4

Aarti¹,

Khusro²,

Agastian³

2017

Bioresour. Bioprocess.

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α‐Amylase and cellulase production by novel halotolerant Bacillus sp.PM06 isolated from sugarcane pressmud

Rajesh

Gummadi

2021

Biotech and App Biochem

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A novel Bacillus sp.PM06 isolated from sugarcane waste pressmud was tested for extracellular α-amylase and cellulase enzyme production. The effect of different substrates, nitrogen sources, pH, and temperature on growth and extracellular enzyme production was examined. Bacillus sp.PM06 was able to grow with starch and carboxymethyl cellulose (CMC) as a sole source of carbon and ammonium chloride was found to be the best nitrogen source. Maximum enzyme production was obtained at 48 H for both α-amylase and cellulase. The optimal condition for measuring enzyme activity was found to be pH 5.5 at 50 °C for α-amylase and pH 6.4 at 60 °C for cellulase respectively. It was found that Bacillus sp.PM06 exhibited halotolerance up to 2 M Sodium chloride (NaCl) and Potassium chloride (KCl). The isolate could produce α-amylase in the presence of 2 M NaCl and 1 M KCl. However, the strain produced cellulase even in the presence of 2 M NaCl and KCl. Concomitant production of both enzymes was observed when the medium was supplemented with starch and CMC. A maximum of 31 ± 1.15 U/mL of amylase and 15 ± 1.5 U/mL of cellulase was produced in 48 H. The enzyme was partially purified by Ammonium sulphate (NH 4 ) 2 SO 4 precipitation with 2.2 and 2.3-fold purification.

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Enhanced production of amylase, pyruvate and phenolic compounds from glucose by light‐driven Aspergillusniger–CuS nanobiohybrids

Uddandarao

Lens

2022

J of Chemical Tech & Biotech

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BACKGROUND:The demand for value-added compounds such as amylase, pyruvate and phenolic compounds produced by biological methods has prompted the rapid development of advanced technologies for their enhanced production. Nanobiohybrids (NBs) make use of both the microbial properties of whole-cell microorganisms and the light-harvesting efficiency of semiconductors. Photosynthetic NBs were constructed that link the biosynthetic pathways of Aspergillus niger with CuS nanoparticles. RESULTS: In this work, NB formation was confirmed by negative values of the interaction energy, i.e., 2.31 × 10 8 to −5.52 × 10 8 kJ mol −1 for CuS-Che NBs, whereas for CuS-Bio NBs the values were −2.31 × 10 8 to −4.62 × 10 8 kJ mol −1 for CuS-Bio NBs with spherical nanoparticle interaction. For CuS-Bio NBs with nanorod interaction, it ranged from −2.3 × 10 7 to −3.47 × 10 7 kJ mol −1 . Further, the morphological changes observed by scanning electron microscopy showed the presence of the elements Cu and S in the energy-dispersive X-ray spectra and the presence of CuS bonds in Fourier transform infrared spectroscopy indicate NB formation. In addition, the quenching effect in photoluminescence studies confirmed NB formation. Production yields of amylase, phenolic compounds and pyruvate amounted to 11.2 ∼mol L −1 , 52.5 ∼mol L −1 and 28 nmol ∼L −1 , respectively, in A. niger-CuS Bio NBs on the third day of incubation in the bioreactor. Moreover, A niger cells-CuS Bio NBs had amino acids and lipid yields of 6.2 mg mL −1 and 26.5 mg L −1 , respectively. Furthermore, probable mechanisms for the enhanced production of amylase, pyruvate and phenolic compounds are proposed.CONCLUSION: Aspergillus niger-CuS NBs were used for the production of the amylase enzyme and value-added compounds such as pyruvate and phenolic compounds. Aspergillus niger-CuS Bio NBs showed a greater efficiency compared to A. niger-CuS Che NBs as the biologically produced CuS nanoparticles had a higher compatibility with A. niger cells.

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Optimization of some fermentation conditions for the production of extracellular amylases by using Chryseobacterium and Bacillus isolates from organic kitchen wastes

Cited by 51 publications

References 29 publications

Goat dung as a feedstock for hyper-production of amylase from Glutamicibacter arilaitensis strain ALA4

Goat dung as a feedstock for hyper-production of amylase from Glutamicibacter arilaitensis strain ALA4

α‐Amylase and cellulase production by novel halotolerant Bacillus sp.PM06 isolated from sugarcane pressmud

Enhanced production of amylase, pyruvate and phenolic compounds from glucose by light‐driven Aspergillusniger–CuS nanobiohybrids

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