Optimization of submerged keratinocyte cultures for the synthesis of barrier ceramides

Dihydroceramide Desaturase Inhibition by a Cyclopropanated Dihydroceramide Analog in Cultured Keratinocytes

“…Moreover, a cell culture model of the human skin is available and allows the analysis of markers of differentiation and proliferation, which should be affected by the presence of sphingolipids with and without 4,5-( E )-double bond. For these reasons we investigated 1 in cultured differentiated human keratinocytes [29]. After seven days of differentiation, the cells were incubated with different concentrations of 1 in the culture medium for 24 hours.…”

“…Differentiation was then induced by submitting the cells to an increased calcium ion concentration in the medium, the so-called calcium shift [29]. Former studies already demonstrated a correlation between mRNA and protein levels under these conditions [36].…”

“…Deviations [%] from levels obtained in untreated cells, which were cultured as previously described [29], are shown.…”

Modeling and validating three dimensional human normal cervix and cervical cancer tissues in vitro

“…In this study, we have developed a 3-D in vitro model to allow in vitro reconstruction of the normal cervix and cervical cancer. Previous studies showed that under conventional culture conditions where keratinocytes were grown in plastic petri dishes or flasks as monolayers, they could only reach an incomplete terminal differentiation and were not able to form multiple cell layers as observed in the human cervix [6-7, 19] . Terminal differentiation of NTERT cells was observed in our multilayered 3-D model of normal cervix.…”

The Role of Epidermal Barrier Impairments in Atopic Dermatitis: Modern Concepts of Disease Pathogenesis

“…Жирные кислоты играют роль в синтезе церамидов и фосфолипидов, которые являют-ся важными компонентами клеточной мембраны [31]. Комбинация трех полиненасыщенных жирных кислот, таких как линолевая, линоленовая и арахидоновая (вита-мин F), является основой для синтеза собственных цера-мидов [32]. При помощи фермента дельта-6-десатураза линолевая кислота превращается в гамма-линолевую, затем в дигомо-гамма-линоленовую [33].…”