Optimization of the aeration and agitation speed of Aeribacillus palidus 418 exopolysaccharide production and the emulsifying properties of the product

Radchenkova, Nadja; Vassilev, Spasen; Martinov, M.; Kuncheva, Margarita; Панчев, И.; Vlaev, S. D.; Kambourova, Margarita

doi:10.1016/j.procbio.2014.01.010

Cited by 41 publications

(31 citation statements)

References 32 publications

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“…In these conditions (i.e., agitation lower than 150 rpm), oxygen was also likely to be the rate-limiting substrate for EPS production. Radchenkova et al (2014) also reported that the EPS production by A. Pallidus was increased by increasing agitation in a 5:1 single impeller jacketed glass reactor with 2.4 L culture medium with maltose as the carbon source. According to Radchenkova et al (2014), agitation was the important factor influencing the availability of nutrients and dissolved oxygen and controlling the rate of metabolite release from the cells.…”

Section: Resultsmentioning

confidence: 90%

“…Radchenkova et al (2014) also reported that the EPS production by A. Pallidus was increased by increasing agitation in a 5:1 single impeller jacketed glass reactor with 2.4 L culture medium with maltose as the carbon source. According to Radchenkova et al (2014), agitation was the important factor influencing the availability of nutrients and dissolved oxygen and controlling the rate of metabolite release from the cells. Bandaiphet and Prasertsan (2006) also observed that EPS production by Enterobacter cloacae WD7 at bench scale was decreased by 44% when the agitation was increased from 200 to 800 rpm.…”

Section: Resultsmentioning

confidence: 90%

“…In aerobic bioprocesses, the EPS production could be enhanced by optimizing agitation (Radchenkova et al, 2014). The agitation is required for the culture to allow mass transfer to occur between the cells and the medium.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Biopolymer Production Kinetics of Mixed Culture Using Wastewater Sludge as a Raw Material and the Effect of Different Cations on Biopolymer Applications in Water and Wastewater Treatment

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Yan²,

Tyagi³

et al. 2016

Water Environment Research

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Thirteen extracellular polymeric substances (EPS) producing bacterial strains were cultivated (as mixed culture) in the sterilized sludge (suspended solids of 25 g/L) and the batch fermentation was carried out. Mixed culture revealed a high specific growth rate of 0.35/hr. The EPS production rate was higher up to 24 hours, which gradually decreased with further incubation. The kinetic estimates demonstrated growth-associated EPS production. Broth EPS revealed higher flocculation activity when combined with different cations (Ca(2+), Mg(2+), Fe(3+), and Al(3+)) in river water (≥90%), municipal wastewater (≥90%), and brewery wastewater (≥80%), respectively. A low dose (5 to 40 mg/L) of trivalent cations was required to achieve higher flocculation compared to the divalent cations (50 to 250 mg/L). Flocculation performance of EPS was comparable to Magnafloc-155 (chemical polymer) and, hence, it could be used as a flocculant.

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Section: Resultsmentioning

confidence: 90%

Section: Resultsmentioning

confidence: 90%

See 1 more Smart Citation

Biopolymer Production Kinetics of Mixed Culture Using Wastewater Sludge as a Raw Material and the Effect of Different Cations on Biopolymer Applications in Water and Wastewater Treatment

More¹,

Yan²,

Tyagi³

et al. 2016

Water Environment Research

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show abstract

“…A maximum EPS yield of 170 μg/mL was measured in a single impeller monoagitator system type Narcissus at agitation speed 900 rpm and aeration rate 0.5 vvm [55]. A maximum EPS yield of 170 μg/mL was measured in a single impeller monoagitator system type Narcissus at agitation speed 900 rpm and aeration rate 0.5 vvm [55].…”

Section: Exopolysaccharides Produced By Bulgarian Thermophilic Isolatesmentioning

confidence: 97%

Thermostable enzymes and polysaccharides produced by thermophilic bacteria isolated from Bulgarian hot springs

Kambourova

2018

Engineering in Life Sciences

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Thermostable enzymes (thermozymes) have been recognized as extremophilic compounds with a greatest biotechnological importance in different industrial areas. Quite recently exopolysaccharides (EPSs) synthesized by thermophiles became an object of increased research interest due to their unique properties appropriate for some specific industrial needs. Thermophilic producers of biotechnologically valuable enzymes and novel EPS were isolated by our group from Bulgarian thermal springs with a diverse geotectonic origin and different water properties. Laboratory reactor processes for their production were developed in batch and continuous cultures. Some of the synthesized thermostable enzymes were among the first described in their groups, for example, the single known thermostable gellan lyase that demonstrated specific activity higher than that of the mesophilic enzymes. Isolated by us thermostable xylanase was able to degrade more than 60% of beechwood xylan in a coprocess with an archaeal β‐xylosidase. Lipase purified by us was active between 55 and 90°C with an optimum at 75–80°C in a large pH range. It was able to degrade a broad range of substrates. Isolates from Bulgarian hot springs synthesized EPS with novel composition and high thermostability. Thus, Bulgarian hot springs harbor a wide set of thermophilic producers of novel enzymes and EPS with potential for a large number of biotechnological applications.

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“…Aeration and agitation were proven to be crucial for attaining maximum productivity in microbial aerobic processes by Aeribacillus pallidus 418 ). According to some authors (Rau et al 1992 ;Radchenkova et al 2014 ), dissolved oxygen limitation is a desirable condition for enhanced polymer production. It is well known that one of the physiological roles of EPSs is cell adaptation to unfavorable conditions; for extremophiles it could serve as enhancer for bacterial survival at severe conditions of extreme niches (Nicolaus et al 2010 ).…”

Section: Specifi C Conditions For Eps Synthesis By Thermophilesmentioning

confidence: 99%

Thermophiles as a Promising Source of Exopolysaccharides with Interesting Properties

Kambourova

Radchenkova

Tomova

et al. 2016

Grand Challenges in Biology and Biotechnology

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Optimization of the aeration and agitation speed of Aeribacillus palidus 418 exopolysaccharide production and the emulsifying properties of the product

Cited by 41 publications

References 32 publications

Biopolymer Production Kinetics of Mixed Culture Using Wastewater Sludge as a Raw Material and the Effect of Different Cations on Biopolymer Applications in Water and Wastewater Treatment

Biopolymer Production Kinetics of Mixed Culture Using Wastewater Sludge as a Raw Material and the Effect of Different Cations on Biopolymer Applications in Water and Wastewater Treatment

Thermostable enzymes and polysaccharides produced by thermophilic bacteria isolated from Bulgarian hot springs

Thermophiles as a Promising Source of Exopolysaccharides with Interesting Properties

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