Optimization of the capillary electrophoresis separation of ranitidine and related compounds

Morris, Vicki M.; Hargreaves, Carol Anne; Overall, Karen L.; Marriott, Philip J.; Hughes, Jeff G.

doi:10.1016/s0021-9673(96)01052-7

Cited by 39 publications

(18 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Structures of these compounds and pK a values are shown in Table 1. Stock standard solutions of each herbicide were prepared in HPLC-grade methanol from Merck (Darmstadt, Germany) at 100 mg?L 21 , and stored in darkness at 247C. Working standard solutions were prepared by diluting with an appropriate volume of methanol until the desired concentration.…”

Section: Chemicalsmentioning

confidence: 99%

“…Data were collected and analyzed using the System Gold software from Beckman. All pH measurements were made using a pH-meter with a glass calomel electrode (PHM 64 Radiometer, Copenhagen, Denmark 21 with a vacuum pump. Then, cartridges were dried under vacuum for 10 min at 20 mm Hg (1 mm Hg = 133.322 Pa).…”

Section: Equipment and Softwarementioning

confidence: 99%

“…With the inclusion of this condition, the design generates information equally in all directions, i.e., a rotation of design about the origin does not alter the variance contours. As experimental response for each electrophoretic run a chromatographic exponential function (CEF) was used, which is an objective function that describes the quality of each experimental run in terms of resolution and duration of the electrophoretic separation [21]: The optimum value resolution, R opt , was set at 1.5, the a value, which adjusts the weighting of the resolution term relative to the time term, was set at 3, and the maximum acceptable time, t max , was set at 40 min. t f is the final peak elution time (in minutes), R i is the resolution of the i th peak pair for each experimental result, and n is the number of expected peaks.…”

Section: Experimental Designmentioning

confidence: 99%

“…Calibration was developed using spiked ground-water samples in the range of 1-50 mg?L 21 and following the SPE procedure described in Section 2.3. For each concentration, three runs with independent samples were carried out.…”

Section: Analytical Characteristicsmentioning

confidence: 99%

See 3 more Smart Citations

Optimization of the separation of a group of triazine herbicides by micellar capillary electrophoresis using experimental design and artificial neural networks

2004

View full text Add to dashboard Cite

The micellar electrokinetic chromatography separation of a group of triazine compounds was optimized using a combination of experimental design (ED) and artificial neural network (ANN). Different variables affecting separation were selected and used as input in the ANN. A chromatographic exponential function (CEF) combining resolution and separation time was used as output to obtain optimal separation conditions. An optimized buffer (19.3 mM sodium borate, 15.4 mM disodium hydrogen phosphate, 28.4 mM SDS, pH 9.45, and 7.5% 1-propanol) provides the best separation with regard to resolution and separation time. Besides, an analysis of variance (ANOVA) approach of the MEKC separation, using the same variables, was developed, and the best capability of the combination of ED-ANN for the optimization of the analytical methodology was demonstrated by comparing the results obtained from both approaches. In order to validate the proposed method, the different analytical parameters as repeatability and day-to-day precision were calculated. Finally, the optimized method was applied to the determination of these compounds in spiked and nonspiked ground water samples.

show abstract

Section: Chemicalsmentioning

confidence: 99%

Section: Equipment and Softwarementioning

confidence: 99%

Section: Experimental Designmentioning

confidence: 99%

Section: Analytical Characteristicsmentioning

confidence: 99%

See 2 more Smart Citations

Optimization of the separation of a group of triazine herbicides by micellar capillary electrophoresis using experimental design and artificial neural networks

2004

View full text Add to dashboard Cite

show abstract

“…Many methods such as polarography [2] differential pulse polarography [3], capillary electrophoresis (CE) [4,5], high-performance liquid chromatography (HPLC) [6 -9], fluorimetry [10], and chemiluminescence (CL) detection method [11] have been developed for ranitidine. Among these methods, the CL method for ranitidine has been given much attention due to its excellent sensitivity, wide dynamic concentration range and the simple instrumental setup.…”

Section: Introductionmentioning

confidence: 99%

Highly Sensitive Electrogenerated Chemiluminescence Detecting Ranitidine Based On Chemically Modifying Microenvironment of the Chemiluminescence Reaction

Zheng

Zhang

et al. 2005

Electroanalysis

View full text Add to dashboard Cite

Using a graphite electrode modified with vaseline and NiO, ranitidine showed a strongly ECL enhancing effect for the weak ECL signal of electrooxidation of luminol. Based on this finding, a more sensitive ECL method for ranitidine was firstly proposed. Under the optimum experimental conditions, the ranitidine hydrochloride concentration in the range of 3.0 Â 10 À8 -9.0 Â 10 À6 mol/L was proportional to the enhancing ECL signal and offered a 9 Â 10 À9 mol/L detection limit for ranitidine hydrochloride. At the same time, based on the investigation on this ECL reaction mechanism, a new concept, to improve the suitable ECL reaction micro-environment with chemically modified electrode technique for the better analytical performances of ECL analysis was also firstly proposed.

show abstract

Simultaneous determination of cimetidine, famotidine, nizatidine, and ranitidine in tablets by capillary zone electrophoresis

et al. 2001

Electrophoresis

View full text Add to dashboard Cite

A simple capillary zone electrophoresis (CZE) method is described for the simultaneous determination of cimetidine (CIM), famotidine (FAM), nizatidine (NIZ), and ranitidine (RAN). The analysis of these drugs was performed in a 100 mM phosphate buffer, pH 3.5. Several parameters were studied, including wavelength for detection, concentration and pH of phosphate buffer, and separation voltage. The quantitative ranges were 100-1,000 microM for each analyte. The intra- and interday relative standard deviations (n = 5) were all less than 4%. The detection limits were found to be about 10 microM for CIM, 20 microM for RAN, 20 microM for NIZ, and 10 microM for FAM (S/N = 3, injection 1 s) at 214 nm. All recoveries were greater than 92%. Applications of the method to the assay of these drugs in tablets proved to be feasible.

show abstract

Optimization of the capillary electrophoresis separation of ranitidine and related compounds

Cited by 39 publications

References 9 publications

Optimization of the separation of a group of triazine herbicides by micellar capillary electrophoresis using experimental design and artificial neural networks

Optimization of the separation of a group of triazine herbicides by micellar capillary electrophoresis using experimental design and artificial neural networks

Highly Sensitive Electrogenerated Chemiluminescence Detecting Ranitidine Based On Chemically Modifying Microenvironment of the Chemiluminescence Reaction

Simultaneous determination of cimetidine, famotidine, nizatidine, and ranitidine in tablets by capillary zone electrophoresis

Contact Info

Product

Resources

About