Optimization of the Culture Medium Composition to Increase the Biosynthesis of Recombinant Human Interleukin-7 in Escherichia Coli

Abstract

The purpose of the work was to rise of biosynthesis of the human recombinant interleukin-7 (rIL-7) in E. coli by optimizing the quantitative and qualitative composition of the culture medium. As a result of optimization of the quantitative composition of organic and mineral components of the culture medium using the matrix of planning a fractional factor experiment, it was possible to increase the synthesis of rIL-7 by 1.3 times. It was proved that the use of supplements to the culture medium in the form of extracts of Clivia miniata and Zephyranthes grandiflora (0.5-1.0%) stimulated the rIL-7 synthesis in recombinant bacterial cells in 1.3-1.4 times. The use of phytoextracts at a concentration of 5.0% and more caused an inhibitory effect on the producer biosynthetic properties. It has been established that the modulation of the biosynthetic activity of recombinant bacteria is due to the interaction of the biologically active substances of plant extracts with the surface structures of the bacterial cell, namely, porins (OmpC and OmpF proteins). The stimulating effect of vitamin K1 (15-25 mg per 1 ml of culture medium) in the liposomal form was proved; such approach allowed increasing the synthesis of rIL-7 in 1.34-1.39 times. And the use of liposomal form of plant extracts as an approach to increasing the bioavailability of biologically active substances is not appropriate.

Impact of using the principle of spectral-dynamic analysis of the intensity of the electret field of the "Vim Vitae" electronic complex on a living organism

“…However, the application of gene therapy remains challenging because of the risk of undesirable effects such as non-target genome changes, toxicity of gene therapy vectors, and immunological responses to transplanted cells or genes. Therefore, further research and development of safer and more effective methods of gene therapy are needed for widespread use in medicine (Collins, 2010;Mertens et al, 2015;Fleischeret et al, 2018;Cross & Burmester, 2006;Ginn et al, 2018;Hauser et al, 2013;Staal et al, 2019;Dai et al, 2016;Marshall, 1999;Naldini, 2015;Datkhile et al, 2021;Giacca & Zacchigna, 2012;Hamblin et al, 2015;Motronenko et al, 2020;Rusyn et al, 2023;Sokol et al, 2020;.…”

Screening of Strains of the Medicinal Mushroom Fomitopsis officinalis (vill.) Bondartsev & Singer Promising for Biotechnological Use

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Stromal-vascular fraction of adipose tissue as an alternative source of cellular material for regenerative medicine

“…Peripheral zone of the graft is the zone of re-generation, where ADSC stimulate the replacement and survival of adipocytes. The highest ration of cell death is observed in the center, being the result of ischemia and poor tissue oxygenation [13,14].…”