Optimization of the fermentation conditions for ethanol production by new thermotolerant yeast strains of Kluyveromyces sp.

Hashem, Mohamed; Zohri, Abdel Naser A.; Ali, Mahmoud

doi:10.5897/ajmr2013.5919

Cited by 28 publications

(6 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Narra et al [43] reported the use of a thermo-tolerant species of Kluyveromyces to hydrolyze and to ferment sugars obtained from cellulosic biomass to bioethanol. The observation in this study was in consonance with the previous report of Hashem et al [44] that mentioned a species of Kluyveromyces was used as the fermenting yeast in the production of ethanol. Other authors such as Saliu and Sani [33] and Abada et al [45] employed Saccharomyces cerevisiae to carry out the fermentation of hydrolyzed cellulolytic biomass to ethanol.…”

Section: Pre-treatment and Saccharification Of Plant Wastes To Hydrolysable Sugarssupporting

confidence: 93%

Saccharification and Fermentation of Cellulolytic Agricultural Biomass to Bioethanol using Locally Isolated Aspergillus niger S48 and Kluyveromyces sp. Y2, respectively

Adeyemo

Ja’afaru

Abdulkadir

et al. 2021

Period. Polytech. Chem. Eng.

View full text Add to dashboard Cite

Due to increase in demand for energy as a result of human population explosion, industrialization and environmental hazards posed by fossil fuels, there is a need to source for alternative energy sources that are cheaper and environmental friendly. Three different lignocellulosic biomasses were studied for their suitability for bioethanol production. Fungi and yeasts were isolated using serial dilution and spread plate methods. Identification of both fungi and yeasts was done using their cultural and microscopy characteristics. Saccharification of the pre-treated biomass was done with both crude cellulase and mycelia inoculant. Bioethanol was produced using batch culture fermentation. Ethanol produced was detected using spectrometric method and quantified using High Performance Liquid Chromatography (HPLC). The effects of substrate concentration, pH and temperature on ethanol yield were optimized. Fifty fungal isolates were obtained from soil collected. Six yeasts, all Kluyveromyces species fermented three sugars to ethanol with isolate Kluyveromyces sp.Y2 having the shortest time. It was selected for fermentation. Aspergillus niger S48 had highest cellulase activity measured in a zone of hydrolysis of 26.0 mm. It had the highest glucanase activity, endoglucanase (0.462 U/mL) and exoglucanase (0.431 U/mL). The outcome of this study indicated that crude cellulase produced by Aspergillus niger S48 hydrolyzed the pre-treated rice chaff with 1.07 mg/mL of fermentable sugars higher than 0.87 mg/mL when the mycelia of the fungus was inoculated to pretreated rice chaff for hydrolysis. Ethanol was optimally produced at 12 % substrate concentration using rice chaff, at a temperature of 35 °C and pH 5.0.

show abstract

Section: Pre-treatment and Saccharification Of Plant Wastes To Hydrolysable Sugarssupporting

confidence: 93%

Saccharification and Fermentation of Cellulolytic Agricultural Biomass to Bioethanol using Locally Isolated Aspergillus niger S48 and Kluyveromyces sp. Y2, respectively

Adeyemo

Ja’afaru

Abdulkadir

et al. 2021

Period. Polytech. Chem. Eng.

View full text Add to dashboard Cite

show abstract

“…Our result is similar with the findings of Raikar (2012), where they observed maximum bioethanol concentration from grape after 48 hrs of fermentation at temperature 35 °C having broth pH 5.0. A similar study by Mohamed et al (2013) observed the highest ethanol concentration of 11.72% at 35 °C Kluyveromyces sp. after 96 hrs of fermentation period.…”

Section: Et Alsupporting

confidence: 68%

Sustainable Utilization of Fruit Wastes for Production of Bioethanol Using Thermotolerant Saccharomyces Cerevisiae Yeast Isolated From Common Fruits of Bangladesh

2023

THE JAPS

View full text Add to dashboard Cite

Bioethanol is one of the most important biofuel due to its positive impact on the environment. However, its economic production is dependent primarily on the raw materials used and the fermentation potentiality of the microorganisms. Therefore, this study was aimed to screen for thermotolerant Saccharomyces cerevisiae yeast isolates from common fruits of Bangladesh and produce bioethanol from decaying fruit wastes. Nine different fruits were collected and subjected for anaerobic growth. Colonies similar to S. cerevisiae yeast were isolated based on cultural (colony shape, size, colour, elevation, surface edge, consistency), morphological (bud, cell shape) and biochemical characteristics (carbohydrate fermentation). Molecular confirmation was done using S. cerevisiae RSP5-C allele specific primer. Six isolates were confirmed as S. cerevisiae based on cultural, morphological, biochemical and molecular characteristics studied. From the four isolates that showed the growth capacity at a higher (41 ºC) temperature than optimum (30 0 C), SC-Gr, isolate from grape, showed the most active growth at 41 ºC and used to produce bioethanol from decaying fruits like banana, papaya, apple and grape. The significantly highest amount of bioethanol concentration (7.92 %) was obtained from the grape waste at 37ºC, at pH 5 after 48 hrs of the fermentation period and the lowest amount of bioethanol (2.37 %) was obtained from decaying banana at 45 ºC and pH 5.0 after 72 hrs fermentation period. This study suggested that decaying fruits could be better utilized in the industry of renewable fuel energy using thermotolerant yeast isolate from grapes.

show abstract

“…marxianus InaCC Y119 exhibited a higher ethanol yield than S. cerevisiae InaCC Y93 at 38°C during 48 h of fermentation. Another study (Hashem et al 2013) reported that Kluyveromyces sp. produces the highest ethanol content at 35°C after 48 h of fermentation.…”

Section: Prehydrolysis Simultaneous Saccharification and Fermentationmentioning

confidence: 99%

Utilization of pretreated oil palm empty fruit bunches and their hydrolysate for ethanol production by Indonesian ethanologenic yeast

ANITA,

OKTAVIANI,

HERMIATI

2023

Biodiversitas

View full text Add to dashboard Cite

Abstract. Anita SH, Oktaviani M, Hermiati E. 2023. Utilization of pretreated oil palm empty fruit bunches and their hydrolysate for ethanol production by Indonesian ethanologenic yeast. Biodiversitas 24: 5243-5252. Oil Palm Empty Fruit Bunches (OPEFB) represent a polysaccharide-rich raw material with promising potential for ethanol production. This study aimed to investigate the ethanologenic yeasts, specifically Saccharomyces cerevisiae InaCC Y93 and Kluyveromyces marxianus InaCC Y119, affect bioethanol production in three different systems: Separate Hydrolysis and Fermentation (SHF), Simultaneous Saccharification and Fermentation (SSF), and Prehydrolysis-Simultaneous Saccharification and Fermentation (PSSF). This work is distinguished by the use of indigenous Indonesian yeast strains, including a thermotolerant strain. In the pretreatment process, 1.13% oxalic acid was added to OPEFB and subjected to microwave treatment at 190°C for 3.01 min. Subsequently, cellulase enzymes (40 FPU/g) and a 10% (w/v) yeast inoculum were introduced into 5.27 g dry weight of pretreated OPEFB pulp. The OPEFB acid hydrolysate was also subjected to fermentation. Ethanol content was monitored at 24 h intervals for 72 h. The PSSF system employs K. marxianus InaCC Y119 at 48 h exhibited the highest ethanol concentration, yielding 0.290 g/g, equivalent to approximately 51.20% of the theoretical yield. Additionally, K. marxianus InaCC Y119 demonstrated its capability to ferment the OPEFB acid hydrolysate into ethanol. These findings underscore the considerable potential of K. marxianus for applications in fermenting both hexose and pentose sugars to produce ethanol within higher-temperature systems.

show abstract

Optimization of the fermentation conditions for ethanol production by new thermotolerant yeast strains of Kluyveromyces sp.

Cited by 28 publications

References 34 publications

Saccharification and Fermentation of Cellulolytic Agricultural Biomass to Bioethanol using Locally Isolated Aspergillus niger S48 and Kluyveromyces sp. Y2, respectively

Saccharification and Fermentation of Cellulolytic Agricultural Biomass to Bioethanol using Locally Isolated Aspergillus niger S48 and Kluyveromyces sp. Y2, respectively

Sustainable Utilization of Fruit Wastes for Production of Bioethanol Using Thermotolerant Saccharomyces Cerevisiae Yeast Isolated From Common Fruits of Bangladesh

Utilization of pretreated oil palm empty fruit bunches and their hydrolysate for ethanol production by Indonesian ethanologenic yeast

Contact Info

Product

Resources

About