Optimization of the medium for streptolydigin production by Streptomyces lydicus AS 4.2501 with precursor feeding using response surface methodology

A combination of microbial strain improvement and statistical optimization is investigated to maximize echinocandin B (ECB) production from Aspergillus nidulans ZJB-0817. A classical sequential mutagenesis was studied first by using physical (ultraviolet irradiation at 254 nm) and chemical mutagens (lithium chloride and sodium nitrite). Mutant strain ULN-59 exhibited 2.1-fold increase in ECB production to 1583.1 ± 40.9 mg/L when compared with the parent strain (750.8 ± 32.0 mg/L). This is the first report where mutagenesis is applied in Aspergillus to improve ECB production. Further, fractional factorial design and central composite design were adopted to optimize the culture medium for increasing ECB production by the mutant ULN-59. Results indicated that four culture media including peptone, K2HPO4, mannitol and L-ornithine had significant effects on ECB production. The optimized medium provided another 1.4-fold increase in final ECB concentration to 2285.6 ± 35.6 mg/L compared to the original medium. The results of this study indicated the combined application of a classical mutation and medium optimization can improve effectively ECB production from A. nidulans and could be a promising tool to improve other secondary metabolites production by fungal strains.

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Mutagenesis breeding of high echinocandin B producing strain and further titer improvement with culture medium optimization

Zou

Zhong

Xia

et al. 2015

Bioprocess Biosyst Eng

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Optimization of Bacillus subtilis cell growth effecting jiean-peptide production in fed batch fermentation using central composite design

Zhong¹,

Xiao-yong²,

Ren³

et al. 2014

Electronic Journal of Biotechnology

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a b s t r a c t 7 a r t i c l e i n f o 8 9 Available online xxxx 10 Keywords: 11 Bacillus subtilis ZK8 12 Iturin A 13 Response surface methodology 14 15Background: Optimization of nutrient feeding was developed to improve the growth of Bacillus subtilis in fed 16 batch fermentation to increase the production of jiean-peptide (JAA). A central composite design (CCD) was 17 used to obtain a model describing the relationship between glucose, total nitrogen, and the maximum cell dry 18 weight in the culture broth with fed batch fermentation in a 5 L fermentor.19 Results: The results were analyzed using response surface methodology (RSM), and the optimized values of 20 glucose and total nitrogen concentration were 30.70 g/L and 1.68 g/L in the culture, respectively. The highest 21 cell dry weight was improved to 77.50 g/L in fed batch fermentation, which is 280% higher than the batch fermen-22 tation concentration (20.37 g/L). This led to a 44% increase of JAA production in fed batch fermentation as 23 compared to the production of batch fermentation. 24 Conclusion: The results of this work improve the present production of JAA and may be adopted for other 25 objective products' production.

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Bioconversion and bioprocess technology for cleaner environment and better life

Chang

2011

Journal of the Taiwan Institute of Chemical Engineers

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Optimization of the medium for streptolydigin production by Streptomyces lydicus AS 4.2501 with precursor feeding using response surface methodology

Cited by 4 publications

References 35 publications

Mutagenesis breeding of high echinocandin B producing strain and further titer improvement with culture medium optimization

Mutagenesis breeding of high echinocandin B producing strain and further titer improvement with culture medium optimization

Optimization of Bacillus subtilis cell growth effecting jiean-peptide production in fed batch fermentation using central composite design

Bioconversion and bioprocess technology for cleaner environment and better life

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