Optimization of the reactional medium and a food impact study for a colorimetric in situ Salmonella spp. detection method

Junillon, Thomas; Mosticon, David; Mallen, Benoît; Baril, Florent; Morand, Lucie; Michel, Déborah

doi:10.1016/j.ijfoodmicro.2014.04.022

Cited by 5 publications

(1 citation statement)

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“…We chose Salmonella enterica ( S. enterica ) as the analytical mode in this assay. S. enterica is a Gram-negative food pathogen that affects the gastroinstinal tract of exposed individuals and leads to infection, diarrhea, and pain. , Infection with S. enterica causes serious foodborne health problems worldwide, especially in developing countries. When the concentration of MB 30 is high (10 μg/mL), the MB 30 captured by the MB 250 -target is much less than the unreacted MB 30 , resulting in a negligible Δ T 2 value.…”

Section: Resultsmentioning

confidence: 99%

One-Step Detection of Pathogens and Viruses: Combining Magnetic Relaxation Switching and Magnetic Separation

et al. 2015

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We report a sensing methodology that combines magnetic separation (MS) and magnetic relaxation switching (MS-MRS) for one-step detection of bacteria and viruses with high sensitivity and reproducibility. We first employ a magnetic field of 0.01 T to separate the magnetic beads of large size (250 nm in diameter) from those of small size (30 nm in diameter) and use the transverse relaxation time (T2) of the water molecules around the 30 nm magnetic beads (MB30) as the signal readout of the immunoassay. An MS-MRS sensor integrates target enrichment, extraction, and detection into one step, and the entire immunoassay can be completed within 30 min. Compared with a traditional MRS sensor, an MS-MRS sensor shows enhanced sensitivity, better reproducibility, and convenient operation, thus providing a promising platform for point-of-care testing.

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Section: Resultsmentioning

confidence: 99%