Optimization of the selectivity of a cyanopropyl stationary phase for the gas chromatographic analysis of trans fatty acids

Martin, Clayton Antunes; Oliveira, Celso Maran de; Visentainer, Jesuí Vergílio; Matsushita, Makoto; Souza, Nilson Evelázio de

doi:10.1016/j.chroma.2008.04.033

Cited by 41 publications

(37 citation statements)

References 29 publications

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“…Fatty acid methyl esters (FAME) were prepared by methylation of total lipids by Maia and RodriguezAmaya (1993). The methyl esters were separated by gas chromatography using a Varian 3300 (USA) gas chromatograph fitted with a flame ionization detector (FID) and a fused-silica CPselect CB-Fame capillary column (100 m x 0.25 mm id., 0.25 µm cyanopropyl CP-7420) (Martin et al, 2008). Injector and detector temperatures were at 240 o C. The column temperature was maintained at 165 o C for 12 min and programmed from 165 to 180 º C at 40 ºC/min for 15 min and from 180 to 240 ºC at 15 °C/min to 18 min.…”

Section: Animal and Dietsmentioning

confidence: 99%

Incorporation of n-3 fatty acids by the liver of mice fed linseed oil as a function of feeding duration

Perini

Stevanato

Visentainer

et al. 2011

Braz. arch. biol. technol.

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Section: Animal and Dietsmentioning

confidence: 99%

Incorporation of n-3 fatty acids by the liver of mice fed linseed oil as a function of feeding duration

Perini

Stevanato

Visentainer

et al. 2011

Braz. arch. biol. technol.

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“…The advancements in gas-liquid chromatography had an impact on the study of fatty acids, contributing, among other things, to the detailed investigation of positional and geometric isomers with distinct biological functions that could not be separated or identified until then. [1][2][3] However, some problems may rise from the esterification of fatty acids, a process necessary for their analysis by GC: incomplete conversion of lipids into FAMEs, alteration of the fatty acid composition during transesterification, the formation of artifacts that may be erroneously identified as fatty acids or overlap with methyl ester peaks in GC analysis, contamination and resulting damage to the chromatographic column due to traces of esterifying Vol. 21, No.…”

Section: Introductionmentioning

confidence: 99%

Validation of the determination of fatty acids in milk by gas chromatography

Simionato¹,

Garcia²,

Santos³

et al. 2010

J. Braz. Chem. Soc.

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Foram analisados os ésteres metílicos de ácidos graxos (FAMEs) em amostras comerciais de leite empregando cromatografia gasosa acoplada ao detector por ionização em chama. Os ácidos graxos saturados (SFA) foram os mais abundantes. Os principais SFA encontrados foram o ácido palmítico (16:0), o ácido esteárico (18:0) e o ácido mirístico (14:0). Foram obtidas diferenças significativas (P < 0,500) entre as quantidades de ácido palmítico (276 ± 17 mg g -1 e 248 ± 20 mg g -1 ) e ácido mirístico (95 ± 5 mg g -1 e 85 ± 7 mg g -1 ) nas amostras analisadas. Entretanto, não foi observada uma diferença significativa para o ácido esteárico (113 ± 6 mg g -1 e 114 ± 11 mg g -1 ). O isômero conjugado do ácido linoleico (CLA) 18:2c9t11 foi detectado nas amostras e quantificado. Para este ácido, as diferenças encontradas nas amostras analisadas não foram significativas (10,4 ± 0,7 mg g -1 e 9,9 ± 0,6 mg g -1 ). A análise de uma amostra do material de referência certificado de leite (RM-8435 NIST) mostrou uma boa recuperação (> 80%) indicando que o método pode ser aplicado para determinar eficientemente ácidos graxos em amostras de leite e em produtos lácteos.Fatty acid methyl esters (FAMEs) in commercial milk samples were analyzed by gas chromatography coupled with flame ionization detection. The saturated fatty acids (SFA) were the most abundant. The major SFA were palmitic acid (16:0), estearic acid (18:0), and myristic acid (14:0). Significant differences (P < 0.500) were found between the amounts of palmitic acid (276 ± 17 mg g -1 and 248 ± 20 mg g -1 ) and myristic acid (95 ± 5 mg g -1 and 85 ± 7 mg g -1 ) in samples. However, no difference was observed for estearic acid (113 ± 6 mg g -1 and 114 ± 11 mg g -1 ). The conjugated linoleic acid (CLA) isomer 18:2c9t11 was detected in the samples and quantified. However, the differences found between the samples analyzed were not significant (10.4 ± 0.7 mg g -1 and 9.9 ± 0.6 mg g -1 ). The analysis of a certified reference powder milk sample (RM-8435 NIST) gave good recoveries (> 80%), indicating that the method can efficiently determine fatty acids in milk and dairy products.

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“…Nevertheless, GC and GC-MS are useful technologies in lipidomics due to the possibility to analyze the sphingoide base and fatty acid chains as derivatization products especially the cis/trans isomers and regioisomers. [37][38][39]. In the field of fatty acid analysis, the resolution ability of GC is much higher than that of LC [40].…”

Section: Gc-msmentioning

confidence: 98%

High‐performance separation techniques hyphenated to mass spectrometry for ganglioside analysis

et al. 2011

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Gangliosides, sialic-acid-containing glycosphingolipids are involved in numerous biological processes and play essential roles in severe pathologies, with predilection in those of the central nervous system. Formerly, ganglioside composition and quantity were assessed exclusively by thin-layer chromatographic (TLC), immunochemical, and immunohistochemical methods, which have limited effectiveness being unable to detect minor components in mixtures of high heterogeneity. Increased awareness of the biological importance of gangliosides stimulated the development of analytical methods that are better amenable to complex ganglioside mixtures. More recently, MS in online conjunction with high-performance separation techniques brought a significant progress to the field. This review highlights the state-of-the-art development and application of separation methods online coupled to MS for ganglioside analysis. Most original and successful protocols based on GC-MS, LC-MS, and CE-MS are presented here together with the special instrumental and sample preparation requirements to be met for effective ganglioside separation, detection, and structural identification. Finally, the advantages and downsides of each methodology as well as the perspectives for simplification, standardization, and upgrading are assessed.

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Optimization of the selectivity of a cyanopropyl stationary phase for the gas chromatographic analysis of trans fatty acids

Cited by 41 publications

References 29 publications

Incorporation of n-3 fatty acids by the liver of mice fed linseed oil as a function of feeding duration

Incorporation of n-3 fatty acids by the liver of mice fed linseed oil as a function of feeding duration

Validation of the determination of fatty acids in milk by gas chromatography

High‐performance separation techniques hyphenated to mass spectrometry for ganglioside analysis

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